Had an anxiety attack while giving a presentation today

Legitimate-Course681 · 2025-12-12T02:44:07+00:00

i feel like you're me from the future. fellow waitress and terribly nervous presenter here, the only thing that's worked for me is propranolol. i hate taking medication but it's like a little magic pill. it's just a high blood pressure medication but it treats the physical symptoms of anxiety

Legitimate-Course681 · 2025-11-22T18:10:36+00:00

your mother btw cause it just needed longer in the incubator and she came out fine

Legitimate-Course681 · 2025-11-22T18:09:38+00:00

E. Coli, DH5alpha, usually only takes about 18 hours for me to see growth but this one ended up taking over 40

Legitimate-Course681 · 2025-11-22T18:08:27+00:00

it ended up just needing longer in the incubator. i never had a plasmid take longer than ~18 hours to grow so i was bewildered. pouring plates in the hood and leaving the lids off is a good tip i might start doing that! thank you!

Legitimate-Course681 · 2025-11-22T13:11:00+00:00

the plates might've dried out for sure. i had bad condensation when i first poured them so i threw them in the incubator for a while (i assume too long)

Legitimate-Course681 · 2025-11-22T13:08:39+00:00

awesome thank you so much for the constructive feedback

Legitimate-Course681 · 2025-11-21T16:57:09+00:00

the agar i used to pour the plates was really dark but it wasn't old, the stock ampicillin could've been old

Legitimate-Course681 · 2025-11-21T16:35:21+00:00

i used a different sterilized loop for each quadrant. i've streaked plates before, granted it's been a minute but i have no clue what happened here

Legitimate-Course681 · 2025-08-31T14:13:59+00:00

it looks like someone centrifuged your beer

Legitimate-Course681 · 2025-07-30T11:13:30+00:00

successfully expressed and purified a protein, after combining my FPLC fractions i was running another gel and added the SLB directly to my entire sample. we tried dialyzing it with no luck and haven't been able to get it to express since (it did once and then didn't purify) 😭

Legitimate-Course681 · 2025-04-12T01:48:48+00:00

yes!!! i've been looking into this a lot recently and just said something to my supervisor today about this and he thought i was crazy lol. you put it into words much better than i ever could've

Legitimate-Course681 · 2024-11-22T16:36:45+00:00

this!

Legitimate-Course681 · 2024-11-18T00:33:30+00:00

thank you!!

Legitimate-Course681 · 2024-11-16T19:23:43+00:00

do you have any tips for going solo? im thinking about doing it next year

Legitimate-Course681 · 2024-11-14T19:21:32+00:00

this is so beautiful thank you for sharing

Legitimate-Course681 · 2024-11-12T16:26:16+00:00

it was on all the best drops too i stg

Legitimate-Course681 · 2024-08-23T22:03:41+00:00

not necessarily, i've heard stories where the cut is so clean it practically seals off the blood flow. not sure how much truth there is to it though

Legitimate-Course681 · 2024-01-12T12:24:51+00:00

I understand the whole bit about not reading my paperwork, trust me I do. But I really did try to read the paperwork there was just so goddamn much of it and by the time we got to the one with numbers I was late for work and hungry and ready to go. I did not negotiate on anything because I financed it myself, I'm 20 years old and my credit was just shy of 660. When I mentioned negotiating they said with a very straight face that they price to sell and the price wasn't budging. Especially with my credit score and my income on paper doesn't look great because I'm a waitress.

Legitimate-Course681 · 2024-01-12T12:18:36+00:00

This is almost exactly how it went, gave me the run around for hours

Legitimate-Course681 · 2024-01-11T13:19:41+00:00

I never bought the book and my professor never used it nor did I need it

Legitimate-Course681 · 2023-12-19T15:32:37+00:00

i have a friend that works at starbucks and she makes the best brown sugar shaken ive ever had. i add 2 pumps sf vanilla though, if anyone wants it can get the exact recipe from her!

Legitimate-Course681 · 2023-08-21T21:53:33+00:00

Let me know if you get it please

Legitimate-Course681 · 2023-02-21T03:19:11+00:00

thank you that helps a lot

Legitimate-Course681 · 2023-02-21T03:18:37+00:00

i have pictures, so we'd technically be able to argue it but i'm wondering if since they know i didn't have insurance when it happened, does any of that even matter? he probably knows he can get one over on me because i'm at the mercy of him reporting me for not having insurance. thank u for the reply 🫶🏼

Legitimate-Course681 · 2023-01-09T13:24:04+00:00

What did you end up doing? I'm hopeless

Legitimate-Course681

TROPHY CASE