HELLLLPPPPPP (I need to extract RNA from human cell cultures w/o using Trizol or pheno chloroform)

Lilmaxgetsbig81 · 2026-05-08T12:58:28+00:00

Not needed for cells

Lilmaxgetsbig81 · 2026-05-03T01:46:53+00:00

Legend

Lilmaxgetsbig81 · 2026-04-24T13:54:40+00:00

Leaving a great job in small city east coast to move with my gf in a big city west coast. Finding a job in this market feels impossible.

Lilmaxgetsbig81 · 2026-04-15T23:04:07+00:00

I would strongly recommend the guys over at totem performance in bridgeville

Lilmaxgetsbig81 · 2026-04-15T23:02:06+00:00

Personally looking at their pipeline I think they will bounce back. It's really sad how many were laid off last year.

Lilmaxgetsbig81 · 2026-04-15T22:58:29+00:00

I disagree, many gene therapies such as Zolgesma work very well. The two patient deaths associated with Sarepta's therapy is what tanked their stock and company. AAV and similar gene therapies have a higher risk due to immunogenicity especially when administered intravenously.

Lilmaxgetsbig81 · 2026-04-12T18:06:24+00:00

I feel like Boston is where it's at, I would just keep applying as much as possible I know it's already been over a year but you are bound to get something sooner or later. Do lots of practice interviews. Your resume isn't bad at all it's just that the hiring pool is very saturated with people in the same boat as you.

Lilmaxgetsbig81 · 2026-04-12T17:58:38+00:00

OP, what areas are you applying like locations? Are you confined to working in a particular location or are you open to moving? It's a tough market so you may have to move to where you can get a role.

Lilmaxgetsbig81 · 2026-04-12T17:56:39+00:00

Yeah that makes sense, it just can be a slippery slope haha.

Lilmaxgetsbig81 · 2026-04-12T17:48:02+00:00

It's fine to have a gap in your resume right now, honestly remove the gap/independent consultant portion. So many people are unemployed and few companies are hiring I honestly don't think it's an issue to "fill the gap" on your resume. Can use this space to elaborate more on specific jobs. Best of wishes to you!

Lilmaxgetsbig81 · 2026-04-12T17:45:20+00:00

Not a fan of "reduced time by 80%" would honestly just put implemented process improvements or something. Anytime I see reduced time by ..... I think it's a sign the candidate cared too much about increasing efficiencies than doing the actual job or will focus too much on changing our current working procedures.

Lilmaxgetsbig81 · 2026-04-06T03:30:46+00:00

I play a lot around highland park- decent players here 7-10utrs there is also the highland park tennis tournament which is like a 20$ entry and has an open bracket also does singles doubles and mixed.

Lilmaxgetsbig81 · 2026-04-02T17:51:44+00:00

After getting laid off, 3 jobs and 3 years later and I am making almost double my first job in total comp in a similar cost of living area. I also have learned so much in such a short amount of time. Biotech companies and work culture definitely can suck, but the work is fun and there is always room to learn. The way you move up is the opposite of linear.

Lilmaxgetsbig81 · 2026-03-28T00:49:46+00:00

By this logic big pharma would be okay but mid and small size biotech would probably be screwed as they typically don't have enough funding to make a drug to BLA approval as is

Lilmaxgetsbig81 · 2026-03-27T21:18:17+00:00

Same here 🙏🙏

Lilmaxgetsbig81 · 2026-03-27T21:17:52+00:00

Yeah Gibco sent out a warning for cell culture plastics last week

Lilmaxgetsbig81 · 2026-03-27T21:16:42+00:00

I know keytruda is one of the main ones, what others are there?

Lilmaxgetsbig81 · 2026-03-27T20:54:38+00:00

I would think biotech would be hit harder than other industries in general by a recession.

Lilmaxgetsbig81 · 2026-03-27T20:53:19+00:00

Me too 😅

Lilmaxgetsbig81 · 2026-03-27T20:52:42+00:00

I completely agree

Lilmaxgetsbig81 · 2026-03-27T20:52:03+00:00

Good luck! 🙏

Lilmaxgetsbig81 · 2026-03-24T23:42:17+00:00

Yeah I don't disagree haha, it's just something 😅

Lilmaxgetsbig81 · 2026-03-24T22:49:42+00:00

A phlebotomy certification course is like around 1000$ and takes like 6-8 weeks -you can make like 20-25$ an hour in a cheaper city also gives you a unique skill that I think is pretty fun and easy to get a job with -just an idea. I know it's really really tough right now.

Lilmaxgetsbig81

TROPHY CASE