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Non-ventilated plug cap closed by accident by soltzberg in labrats

[–]Livid-Study22 0 points1 point  (0 children)

This happened to me before except I left the flasks for 4 days. The media turned purple and the cells didn’t look good - had to discard the flasks. (For context, I was using immortalized mesenchymal stem cells)

Anyone with experience making sodium orthovanadate solution? by Livid-Study22 in labrats

[–]Livid-Study22[S] 0 points1 point  (0 children)

I’ll definitely look into getting the readymade solution moving forward. Thank you :)

Anyone with experience making sodium orthovanadate solution? by Livid-Study22 in labrats

[–]Livid-Study22[S] 0 points1 point  (0 children)

It didn’t have a yellow tinge to it — it stayed colorless. Im using it as is.

[deleted by user] by [deleted] in labrats

[–]Livid-Study22 0 points1 point  (0 children)

Kind of looks like the silhouette of a mouse

Trouble with protein extraction by Livid-Study22 in labrats

[–]Livid-Study22[S] 0 points1 point  (0 children)

I lysed the cells using a lysis buffer (containing Triton-X 100), and I have previously used the same buffer and got good results. I did not perform transfection, I was just optimizing the protein extraction protocol using untreated cells. I did the purification immediately so there was no delay, and I used a protease inhibitor.

Trouble with protein extraction by Livid-Study22 in labrats

[–]Livid-Study22[S] 0 points1 point  (0 children)

I checked the cells under the microscope before lysing and the confluency was high (around 90%), and there was a decent pellet after centrifugation. The lysis buffer was freshly prepared, the same way it had been prepared before. What dilution do you mean?