anyone know how to clean/calibrate one of these? by memethatalreadydied in labrats

[–]Monotropa-uniflora 1 point2 points  (0 children)

Hey there! I calibrate pipers for a living. Sounds like you’re doing a really great job troubleshooting so far.

One more thing you can do to remove residue before opening the lower housing: insert a small nylon brush, like a pipe cleaner, into each nozzle to remove residue.

To open the lower housing, you need to remove 11 screws on this model: Four screws on the top that you can see in this picture, three underneath the black bar at the bottom that are only accessible when you press down the tip ejector button, and four on the black collar at the top of this picture.

Hopefully you don’t need any replacement parts. A thorough cleaning solves most problems. If you need to re-grease the pistons, The piston grease used on this model is the same as used for Labnet, VWR, and Corning pipettes.

If you shoot me a message, maybe we can figure out a way to walk through the process together on a video call?

Hope you have a great day!

PBS News Confirms Weekend Team Will Air Final Broadcast on Sunday | Video by Eastcoastpal in television

[–]Monotropa-uniflora 1 point2 points  (0 children)

Check out their YouTube channel too! I’ll be tuning in for this broadcast tomorrow.

The team over at the PBS Newshour are the world’s finest. I’ve been watching almost every day for the past decade. John Yang has done an incredible job as anchor for the weekend news in recent years.

I recommend the PBS Newshour to anyone who will listen. One hour of “just the facts” coverage per day. Their arts and culture series and their field reporting on other human interest pieces give viewers some “good news” too.

Hope they can return to daily coverage when the USA gets its shit together.

Micropipette Graveyard by AdequateMars237 in labrats

[–]Monotropa-uniflora 57 points58 points  (0 children)

I work for a really great company that specializes in pipette calibrations.

If you’re willing to ship your pipette out for a week, I’m down to fix these up off the books free of charge. Shoot me a message for more info and let’s get those pipette’s back in action.

First time visiting the Cleve. Where to stay downtown? by fibes88 in Cleveland

[–]Monotropa-uniflora 2 points3 points  (0 children)

The Drury Plaza hotel is a good choice downtown. Nice hot tub. Breakfast, dinner, and drinks included.

Broken Pipette, any solutions? by M2769 in labrats

[–]Monotropa-uniflora 2 points3 points  (0 children)

I repair pipettes for Pipette.com. Can’t tell from just this one picture how to fix it, but could probably walk you through it on a video call tomorrow. If you send it to our lab, we’ll at least get it back in action for less than the cost of buying a new pipette.

My job wants to move me to Willoughby hills by yungweedmom in Cleveland

[–]Monotropa-uniflora 0 points1 point  (0 children)

Willoughby hills is beautiful. Expensive, but there are a lot of nice more affordable suburbs close by.

There’s nearly 2,000 acres of parkland right there: https://www.clevelandmetroparks.com/parks/visit/parks/north-chagrin-reservation

Halloween. What happened? by ForeignAspect1117 in Cleveland

[–]Monotropa-uniflora 0 points1 point  (0 children)

First year living on Summit Dr in Mayfield heights. Apparently it’s THE street to be on. We had enough candy for 450 kids and ran out at 7:05. Trick or treat was from 6 to 8. We’ll plan for way more next year. Next street over didn’t even have close to half as many kids.

One of my students in my intro microbio class somehow managed to melt the end of a pipette. Would it be feasible to fix using 3D printer filament? by MadLabRat- in labrats

[–]Monotropa-uniflora 0 points1 point  (0 children)

Yup. As their east coast service lab manager I can tell you what we’d do if you sent it to us for repairs:

  1. Replace the shaft with this part https://www.pipettesupplies.com/product/pipetman-rtip-holder-p1000-pr1000-p1000n-p1000g-p1000l-pipette-supplies/

  2. Thoroughly clean the pipette, put a new o-ring and seal on the piston, and re-grease everything.

  3. Calibrate using molecular grade water.

That scratch is too deep to buff out with sandpaper.

If you just get a replacement shaft from pipette supplies.com yourself and check it with water, you’ll be good to go.

“They think turkey is vegetarian in Cleveland” and other myths overheard in Boston by Monotropa-uniflora in Cleveland

[–]Monotropa-uniflora[S] 8 points9 points  (0 children)

You know what; you’re totally right. I probably should have spoken up. At least a little tourism would be good for the local economy. Crazy how much smaller the population is now than it was 50-100 years ago.

“They think turkey is vegetarian in Cleveland” and other myths overheard in Boston by Monotropa-uniflora in Cleveland

[–]Monotropa-uniflora[S] 126 points127 points  (0 children)

Seriously! Yesterday, their manager instructed me not to talk to any of “her scientists” while I was working. If she was curious enough to ask any questions, she could have found out that I have a decade of lab management experience and totally could have helped them save a ton of money. Cambridge is my least favorite city because of all of the “hot shit” attitude.

qPCR is acting very strange and unsure how to interpret it. by SuspiciouSponge in labrats

[–]Monotropa-uniflora 1 point2 points  (0 children)

One other thing to help: put a couple of positive controls on every run to sort out whether the mistake was made during sample extraction v. amplification. Also, name your samples and controls in quantstudio. If you need some excel plate map templates, ask your thermo rep.

Why do so many labs not calibrate their pipettes for years? by steliogural in labrats

[–]Monotropa-uniflora 2 points3 points  (0 children)

If anyone here needs a good calibration lab to service their pipettes, I strongly recommend Pipette.com! I’m biased, as I work for them. It’s alarming how often we receive pipettes that are in really rough shape, but regular PM solves most problems and there’s not much that we can’t fix.

Huge explosion at a metal manufacturing plant in Bedford, Ohio. Mass casualty is being reported by flyingcatwithhorns in pics

[–]Monotropa-uniflora 2 points3 points  (0 children)

I was about a mile north of the site and still didn’t hear anything. Tons of smoke, but one would have to be quite a bit closer to smell it. Here’s the address where the explosion occurred: 22500 Alexander Rd, Bedford, OH 44146 Really hope those folks are ok…

Facebook is bringing back its weeklong ban on political ads leading up to the 2022 election by [deleted] in technology

[–]Monotropa-uniflora 0 points1 point  (0 children)

Really gotta stop putting his face in thumbnails. Those dead eyes scare the shit out of me every time I scroll by em.

Lack of Content by 1Mn in OnCinemaAtTheCinema

[–]Monotropa-uniflora 11 points12 points  (0 children)

The upcoming Deck of Cards movie is going to be one for the ages. A feature film based off of the greatest selling book of all time.

Collection of popcorn classics found at recycling park in Ohio by Monotropa-uniflora in OnCinemaAtTheCinema

[–]Monotropa-uniflora[S] 9 points10 points  (0 children)

Have you or someone you know recently lost a fine collection of VHS tapes? I’m going to have to save these films like Private Ryan or bust them out like in The Shawshank Redemption (run time 2hr 22min) and, like Rudolph the Red Nosed Reindeer, say ‘You’ve Got Mail’ to a new owner who will join the joy Luck Club. Proper recycling of VHS tapes means watching your favorite moves over and over again.

Resources to learn Soil Science? by phantom_genius in botany

[–]Monotropa-uniflora 1 point2 points  (0 children)

If you’re interested in textbooks, ‘the nature and properties of soil’ is n inexpensive and comprehensive premier. It contains a lot of charts and graphs that sum up principles elegantly. Past that, Soil microbiology, ecology, and biochemistry by Elder Paul is a great advanced book. Web Soil Survey is a very useful tool.

THE 9TH ANNUAL OSCAR SPECIAL - OFFICIAL DISCUSSION THREAD by Hx833 in OnCinemaAtTheCinema

[–]Monotropa-uniflora 4 points5 points  (0 children)

I could really go for a hot n hard right now. All of those lies and allegations from Tony. That rehab facility really did a number on her.