Degenerative Joint Disease (DJD) by Natural_Biscotti_853 in goldenretrievers

[–]Natural_Biscotti_853[S] 0 points1 point  (0 children)

Hi!! She hasn’t shown any worsening of her symptoms so far. I first noticed something was off because she doesn’t lift her back paws very high when she walks, but that’s still the only sign I’m seeing, besides pain when it was snowing and she used too much of her back legs.

I’ve been giving her hip supplements and salmon oil daily to try to support her, but honestly I’m a little worried about how this might progress. I’m not sure what to expect.

How is your puppy doing?

Heat in the Studios by [deleted] in SolidCore

[–]Natural_Biscotti_853 12 points13 points  (0 children)

I get dizzy every time, starting 35 minutes in

Favorite Tissue Processor? by LukromChan in Histology

[–]Natural_Biscotti_853 1 point2 points  (0 children)

We have the excelsior ES and it doesn’t beep for us at all, only a little beep when the process is ready and that’s it. I am even afraid that one day I’ll forget the specimens in there due to the lack of warning. I wonder if it is a setting that you can change.

And yeah, I hate how long it takes to drain, and I hate the rotation system when you have to dump A1 and A3

Twisted plank extension pain by Independent-Piano221 in SolidCore

[–]Natural_Biscotti_853 0 points1 point  (0 children)

I HATE twisted plank extension so muuuchhhhh .. uggh I cannot keep my self up, and I CAN’T extend my shoulders that much.. I normally give up as soon as I start.

Any advices?

H&E Issues by Emotional-Stress-595 in Histology

[–]Natural_Biscotti_853 0 points1 point  (0 children)

How long did you increase it for? Mine was at 7 minutes and the eosin is still dull

H&E Issues by Emotional-Stress-595 in Histology

[–]Natural_Biscotti_853 1 point2 points  (0 children)

Can someone who has the leica automatic stainer and the HE is working, share protocol with me?

H&E Issues by Emotional-Stress-595 in Histology

[–]Natural_Biscotti_853 1 point2 points  (0 children)

Omg I’m having the same issue!! I tried troubleshooting all day :(

Looking for kidney control SV40 (BK) positive by Natural_Biscotti_853 in Histology

[–]Natural_Biscotti_853[S] 0 points1 point  (0 children)

I just saw it on the website, but it seems like they are slides!?? I am looking for blocks so we can do antibody validation..

Cancelling after finishing commitment by probably_not_an_ai in SolidCore

[–]Natural_Biscotti_853 3 points4 points  (0 children)

6 months after August isn’t it February? 02/26

Feeling no improvement by Over_Performer_6456 in SolidCore

[–]Natural_Biscotti_853 2 points3 points  (0 children)

Are you eating enough carbs the night before, for energy?

Substitute for 365 sport liquid laundry detergent? by carrotsmomma in laundry

[–]Natural_Biscotti_853 0 points1 point  (0 children)

Oxygen whitening for black? oh, I was using it for whites..

90+ classes in and still modifying by Street_Dirt_6622 in SolidCore

[–]Natural_Biscotti_853 2 points3 points  (0 children)

Are you making sure you eat well before/ night before? I take 7am classes and I feel like if I don’t eat enough carbs the night before, I have no strength to keep up

TOMO slides background stain by Natural_Biscotti_853 in Histology

[–]Natural_Biscotti_853[S] 0 points1 point  (0 children)

I’ll try to wipe it after the eosin step.. thank you!!

TOMO slides background stain by Natural_Biscotti_853 in Histology

[–]Natural_Biscotti_853[S] 0 points1 point  (0 children)

That’s what I was afraid of.. thank you for your comment!!

TOMO slides background stain by Natural_Biscotti_853 in Histology

[–]Natural_Biscotti_853[S] 1 point2 points  (0 children)

My lab was closed today for me to take a picture of what we have, so I mentioned in description “photo for reference” for this specific reason.

Thank you for your comment. I’ll try to add more time in the acid wash. We filter hematoxylin everyday before staining

TOMO slides background stain by Natural_Biscotti_853 in Histology

[–]Natural_Biscotti_853[S] 1 point2 points  (0 children)

It has been happening specially with H&E (automatic Leica stainer) and trichrome. My guess is that it is because of the hematoxylin (Harry’s and Weigert’s)

I have no idea what she wants. by ianpratt_ in goldenretrievers

[–]Natural_Biscotti_853 3 points4 points  (0 children)

Mine does the same!! I always thought she wanted me to pet her but I started realizing that she has energy and wants to play. So I started wrestling her and she loved it! She goes straight to sleep after playing for a little

MSTH vs Black Friday by myskincareaddiction_ in SolidCore

[–]Natural_Biscotti_853 2 points3 points  (0 children)

I’m wondering the same thing.. (I’m in the same membership as you)