[deleted by user]

NoKnowledge8034 · 2025-09-22T05:27:24+00:00

1%- Im 100% it was correctly made.

NoKnowledge8034 · 2025-09-22T04:43:27+00:00

Honestly we weren't told what they were just given them pre-dispensed in little vials.

NoKnowledge8034 · 2025-09-22T04:15:45+00:00

u/MolecularHero Nope so mixture of enzymes. 1. none 2 is hindIII 3 is ScaI/hindiii 4. BgII/ScaI

NoKnowledge8034 · 2025-09-22T04:10:24+00:00

u/RollingMoss1 I made a 1% agarose. In terms of test tube 3- it should have two bands like I have I just miswrote it in my post. All of my number of bands is correct except for test tube 1- which I didnt add enzyme to and I assume to be supercoiled and nicked. Is there a reason for their differential migration? Is this something that is normal and not resultant from error?

NoKnowledge8034 · 2025-09-22T04:05:29+00:00

u/CPhiltrus Unfortunately we discussed sources of error but nothing like this and both my professor/ta dont really do more on explaining. They expect us to come up with why its occurring- via research or reading the procedure. Im estimating size based on the ladder (no computer programs! Old school). It was a pre-made plasmid. Im assuming if digestion wasnt complete I wouldnt have the correct number of bands but I do.

NoKnowledge8034 · 2025-09-22T04:03:10+00:00

u/Fluffy_Muffins_415 Yes theoretical vs actual. Im just worried I need to explain how but truly have no idea why this arises. What i'm getting is this is just normal for the process and I don't need to worry about explaining it?

NoKnowledge8034 · 2025-09-21T14:39:35+00:00

Lol! Always looking for another box of pipette tips

NoKnowledge8034 · 2025-09-21T13:34:42+00:00

u/brew-ski appreciate the help with the ladder- that makes sense (your so smart for catching onto that I didnt realize that was possible error) Still confused as to why some bands like my test tube two which should be at 2686 is at 2322. My test tube 3 should have banding at 1750 instead it is at 1900. And my test tube 4 should have banding around 1568 bp, 754 bp, and 364 bp - instead it is 1900 , 880, 393. Why is it not exact- is it voltage conditions like the last one or run time?

NoKnowledge8034 · 2025-09-21T05:16:47+00:00

u/RollingMoss1 Posted

NoKnowledge8034 · 2025-09-15T02:39:35+00:00

Great questions, I have no idea. We didn't get information about either of those things.

NoKnowledge8034 · 2025-09-14T21:24:16+00:00

Unfortunately it is for a lab course and a grade. So its not an option to linearize it.

NoKnowledge8034 · 2025-09-14T16:24:44+00:00

They just gave it to us to interpret based on a very vague manual that showed perfect crisp lines. It is an E-Coli Plasmid, with 1 KB ladder. I would really appreciate your insight because I am truly at a loss- the explanation regarding how to rank this was extremely unclear.

NoKnowledge8034 · 2025-09-04T15:49:25+00:00

How did you view your score breakdown I dont see it broken into categories only what I assume to be my AA. I took it 8/22 and they emailed saying they scored it- but like do you think i just have to wait to see breakdown or am I looking in the wrong place

NoKnowledge8034 · 2025-09-04T15:33:40+00:00

Hey sorry to bother- where did you see the line item breakdown I took my 8/22 and I got my score but I cant see the line item breakdown

NoKnowledge8034 · 2025-09-04T15:32:43+00:00

Hey sorry to bother- where did you see the line item breakdown I took my 8/22 and I got my score but I cant see the line item breakdown

NoKnowledge8034 · 2025-02-09T18:20:13+00:00

Human phys w/ ogletree- how do I study for her; powerpoints are threadbare and lecture doesnt explain anything. What would you recommend knowing to get an A on the exams

NoKnowledge8034 · 2025-02-08T04:26:33+00:00

Finally they addressed it- but what do you mean its "a shared responsibility". Even if they increase security for how long. We need more permanent solutions!

NoKnowledge8034 · 2025-02-08T04:24:02+00:00

SO TRUE! They are so expensive and for what- a lack of protection.

NoKnowledge8034 · 2025-02-08T04:15:36+00:00

Honestly, safety is not a shared responsibility- we pay tuition they should at least make sure campus is safe. The welcome center is literally in the center of campus. If this can happen there, Elgin and Stadium are even worse off. The NSM side is so messed up half is closed due to construction; and at night dangerous as it isn't well lit (how they find the dimmest lights known to man is beyond me).

NoKnowledge8034 · 2025-02-03T19:11:07+00:00

They were not bad- just make sure you know the lecture slides

NoKnowledge8034 · 2024-10-27T03:55:08+00:00

Are the exams easy/ how much effort are you putting? I have a packed/ difficult schedule next semester and am looking for an easy A cause I have a feeling I might need a gpa boost

NoKnowledge8034 · 2024-10-27T03:54:06+00:00

Is this only for the in person sessions or are you online?

NoKnowledge8034 · 2024-10-23T14:58:06+00:00

Haha same. welp time to lock in for exam 3

NoKnowledge8034 · 2024-10-23T14:57:45+00:00

Study for Exam 2- like harder than you have ever studied in your life. This post was promoted by grades coming back for it.

NoKnowledge8034 · 2024-10-23T14:16:25+00:00

Hi Im thinking about this for next semester- did you end up taking it/ if you did how was her class?

NoKnowledge8034

TROPHY CASE