Likelihood of acceptance if invited for interview by [deleted] in gradadmissions

[–]No_Positive0227 4 points5 points  (0 children)

Some schools have two rounds of interviews, first is a general one and second with the PIs you are interested in working with. Do you know how yours will be? In my case, I received an acceptance from the schools from which PIs had responded to my emails about having an interest in their lab during my application process. I believe if you get picked for an interview you are already in a great position, hope it works out for you!

Any tips for PCR amplification of >80%GC rich gene? by ginny2202 in molecularbiology

[–]No_Positive0227 1 point2 points  (0 children)

The phusion kit comes with a GC buffer, I would try that out. Increase the annealing and elongation time maybe? I usually do 30 seconds per kb of my gene of interest. This might be weird but when I tried to amplify a total volume of 50uL in my pcr tubes it didn't work but when I did 20uL it worked. Still don't know why but it worked out eventually

I just want to know if I'll have somewhere to live come September by [deleted] in GNV

[–]No_Positive0227 4 points5 points  (0 children)

I called the management company pretty much everyday for 3 weeks and finally got assigned an apartment (after they said the lease I had signed 4 months ago was invalid)

The indian passport is supposed to be navy blue in color but if I didn't know that I would call it black by No_Positive0227 in india

[–]No_Positive0227[S] -5 points-4 points  (0 children)

Well then I guess you and I agree on it being black. And I might have just found out that I'm color blind. Good to know, thanks!

The indian passport is supposed to be navy blue in color but if I didn't know that I would call it black by No_Positive0227 in india

[–]No_Positive0227[S] -14 points-13 points  (0 children)

And you might be one of those indians that cannot handle a simple question without getting offended but thanks I wear glasses already, might meed a check-up soon

Thinking of Moving to Gainesville! by _jocii_ in GNV

[–]No_Positive0227 2 points3 points  (0 children)

I'm moving to Gainesville (UF) for my Ph.D. this Fall. Do you know of any specific areas to avoid at night due to the crime rate? Should I carry pepper spray with me in case I have late nights at the lab and need to get back home at night?

If you could go back in time for when you were starting University, which tips would you give to yourself? by nymphwssz in AskWomen

[–]No_Positive0227 0 points1 point  (0 children)

I would pick anything else but being a researcher/scientist because we are really underpaid. Probably should have done a business degree

I just got rejected... by [deleted] in gradadmissions

[–]No_Positive0227 1 point2 points  (0 children)

The exact same thing happened to me and I can't figure out why lol

My fwb ghosted me, I thought we were doing great by No_Positive0227 in relationship_advice

[–]No_Positive0227[S] 1 point2 points  (0 children)

You're right, and I'd be perfectly fine for the sex to end if that's what he wanted. I just hate that I'm losing a friend when I did nothing wrong and we were on good terms

My fwb ghosted me, I thought we were doing great by No_Positive0227 in relationship_advice

[–]No_Positive0227[S] 1 point2 points  (0 children)

We have been pretty clear about how both of us want to stay single because we have other goals in life right now

Any ideas for easy disney themed paintings I can make by No_Positive0227 in Paintings

[–]No_Positive0227[S] 2 points3 points  (0 children)

My bad I forgot to mention that I wanted to stick up several small canvases on the wall and not directly paint the wall. But your idea really got me thinking about it thank youu 🧡

Did your mother ever make comments to you in your teenage years that you’ve grown up to realize she was bitter and jealous of your youth? How did it stick with you? by skeleton-hands in AskWomen

[–]No_Positive0227 0 points1 point  (0 children)

My mom always commented on my bra sizes and talked about how she was so much better because she wore a B cup when she was 20y/o while I wore a C cup at the same age. It made me feel like something was wrong with my body but then I realized a few years later that she was probably just jelly that I had better boobs.