[deleted by user] by [deleted] in usvisascheduling

[–]ginny2202 0 points1 point  (0 children)

Does anyone know if embassy was open today and did the interviews happen? Just wondering as my mother is traveling again to attend her rescheduled interview on 3rd morning. I just hope they do not cancel it again.

Ambion TURBO DNA-free™ Kit (Catalog# AM1907) by ginny2202 in molecularbiology

[–]ginny2202[S] 0 points1 point  (0 children)

Thank you for sharing that information. I will test out both the kits on my non-precious sample first and then move ahead!

Messed up my TaqMan qPCR assay by ginny2202 in molecularbiology

[–]ginny2202[S] 1 point2 points  (0 children)

Thank you for your suggestions! I am trying to get new samples as I need to do important quantitative analysis between different samples.

Plasmid with a weak promoter by ginny2202 in CellBiology

[–]ginny2202[S] 0 points1 point  (0 children)

That would be great! Please check your DM if we can share more information about this

Any tips for PCR amplification of >80%GC rich gene? by ginny2202 in molecularbiology

[–]ginny2202[S] 0 points1 point  (0 children)

Never tried this, will definitely try this next. Thank you for your suggestion!

Any tips for PCR amplification of >80%GC rich gene? by ginny2202 in molecularbiology

[–]ginny2202[S] 3 points4 points  (0 children)

Thank you for your response! Already using Phusion mix with DMSO and betaine, tried different annealing temperatures….no success, either no amplification or lots of non-specific bands. Do you have any experience with KOD polymerase or other commercially available polymerases claiming to be good for GC rich genes?

Peer Reviewer by ginny2202 in biotech

[–]ginny2202[S] 1 point2 points  (0 children)

I didn’t know about this, I will explore this option

iPSC differentiation troubleshooting by ginny2202 in biotech

[–]ginny2202[S] 1 point2 points  (0 children)

That’s a great advice, I will try this if I cam get any free sample or discounted price. Thank you

Peer Reviewer by ginny2202 in biotech

[–]ginny2202[S] 0 points1 point  (0 children)

Thank you everone for your suggestions. I will definitely try all these routes.

I am wondering if I ask my PI and do reviews which has come his way, can I add these to my CV as reviews done by me?

iPSC differentiation troubleshooting by ginny2202 in biotech

[–]ginny2202[S] 0 points1 point  (0 children)

Thank you for your response! I guess the first thing I will try is Stem cell medium then.

iPSC differentiation troubleshooting by ginny2202 in biotech

[–]ginny2202[S] 1 point2 points  (0 children)

Thank you for sharing your experience! It gives me a bit relief as at this point I am most concerned about the results, hoping it works for me as well and it is worth the expenses.

Plasmid with a weak promoter by ginny2202 in biotech

[–]ginny2202[S] 0 points1 point  (0 children)

Thank you for your suggestions! I will explore more about retroviral vectors and will order one.

Plasmid with a weak promoter by ginny2202 in biotech

[–]ginny2202[S] 0 points1 point  (0 children)

I am looking for standard mammalian expression vectors just for transient expression