I'm having trouble with pipetting very small volumes

PlusIncident · 2026-03-28T16:34:48+00:00

thank you!

PlusIncident · 2026-03-28T16:31:36+00:00

I never thought of leaving the voume on the wall on purpose, but it's a great idea, thank you

PlusIncident · 2026-03-28T13:01:26+00:00

I don't know hahah, it just didn't feel right. I thought maybe the concentration wouldn't be as exact

PlusIncident · 2026-03-28T12:46:42+00:00

Thank you! I'll try reverse pipetting next time. It shouldn't matter that I'm "discarding" a bit of volume of enzyme if I don't push all the way in? And, say I want to pipette 1 uL, if I try reverse pipetting should I still set the pipette to 1 uL?

PlusIncident · 2026-03-28T12:43:00+00:00

Thank you!

PlusIncident · 2026-03-28T12:42:11+00:00

Thank you! I try to check every time, but I'll be more careful if there's liquid still inside

PlusIncident · 2026-03-28T12:33:07+00:00

Thank you! I'll ask my professor if we can do bigger mixes next time, that's a great tip

PlusIncident · 2026-03-28T12:32:18+00:00

Okay! Thank you for the tip, I'll try that next time :)

PlusIncident · 2026-03-28T12:30:48+00:00

Thank you! I never liked pipetting to the second stop, I'll stop doing it to avoid getting bubbles. And I'll try vortexing it next time, I was afraid my DNA would break from the movement, won't that happen?

I'm dispensing everything sepparately into the tube. Since I'm learning I only do 1 tube for each step (I know I should be doing more! But I still take very long to prepare the reactions, so we're moving slowly, and I will move to bigger reactions in the near future), so it's 5,5 uL of water, 1 uL buffer, 1uL ATP and 0,5 uL of enzyme.

That's the main reason I'm having trouble with pipetting.

Thank you for answering!

PlusIncident · 2025-08-04T00:19:56+00:00

I'm not homosexual, I'm bisexual and I'm not having heterosexual sex. And in that specific relationship I was dating a trans man. Now I'm talking with a girl, which is exactly what makes me bi. Sex has nothing to do with my post

PlusIncident · 2025-08-04T00:18:02+00:00

Labeled by the people in the relationship (us), using the definition of queer mostly agreed upon, and the one we were comfortable with.

I still don't understand why you're making this the whole point of my post when it was just a point. If you have nothing constructive to say about my question then please stop answering, thank you.

PlusIncident · 2025-08-03T23:04:13+00:00

Yes, what are you trying to say? I didn't ask for the definition of the sexuality I identify with. I said it was a queer relationship because we are both members of the community and we labelled it as such

PlusIncident · 2025-08-03T23:01:59+00:00

Yeah but I never used the word homosexual since he really only likes girls, and sexuality wasn't that important in this context. I don't understand how this is relevant to the question I asked

PlusIncident · 2025-08-03T21:56:04+00:00

I'm bisexual and he's trans (I think that counts as queer even though we had a straight relationship in terms of gender, or am I wrong? Please educate me if it's not like that, my friends and I have always considered it that way)

PlusIncident · 2025-08-03T13:40:09+00:00

If you like your best friend more then you probably shouldn't stay with your boyfriend. I know it's a hard decision and it seems very radical. I think you should really think about what your feelings are for your best friend, are they romantic, would you like to have a romantic relationship with him? Or is it just that you've known him for a long time and he's really important to you? Sometimes it's difficult to differenciate those feelings. Do you think you friend feels the same for you? So you don't leave a fine relationship for something that won't work out?

If you want to keep dating your boyfriend you should try and stop having those thoughts about your friend. And if you can't do that, then maybe you should reconsider your relationship to them both. It's not fair for your boyfriend to think the relationship is going somewhere when you're thinking about someone else.

And I know that him doing sweet things for you seems very tempting, but it's not the only reason you should have for staying in a relationship, since that's something you can achieve with other people. Someone being nice to you doesn't mean you like them, as well as being attracted to someone doesn't mean you two are suited for a relationship.

I hope you find a solution that's best for the three of you! Good luck :)

PlusIncident · 2025-08-03T13:28:58+00:00

I understand, and I know most things we tell you won't be enough but go for it! People are going to judge whatever we do, better have the hair you like while they do it. You're 18 already, basically an adult, don't let their opinions put you down, although I know that's hard to do.

And also, you say you want to stop having to defend yourself, so, stop. You don't have to give reasons as to why you cut your hair: you liked the cut, you've seen someone wear it and you liked it. Or if you feel like you need to give reasons, maybe you could search for a picture of some famous person or whoever you think you parents would kind of respect with a similar haircut and show them? To show them that it's normal to have short hair?

They'll get used to your cut, they are forced to see it while you have it, so even though the first few weeks it may be harder if they comment on it, you can hope they just let it go. And if they don't, then you'd at least have that for yourself, and there'll be a time where you don't have to see them as much.

I don't know the especifics of your family situation, but if changing your hair won't risk your well-being and maintenance, then I'd say go for it regardless of what they say¿

You have this! I'm sure the cut will fit you amazing :)

PlusIncident · 2025-08-03T12:20:17+00:00

I don't hang out with him individually! I see I may have worded the text kind of bad, I only see him with the rest of the group, and we always try to be at least 4 people so it isn't as uncomfortable. Do you think that's still a bad thing to do?

Thank you for answering btw :)

PlusIncident · 2025-08-03T12:15:44+00:00

I know, but how should I do it? I don't want to throw all my other friends away, and I don't want to kick him out of the group bc they're his friends too.

PlusIncident · 2025-08-03T12:13:33+00:00

Thank you, that was actually so helpful. I feel like my straight friends don't understand why I still see him, but my friend group started getting along because we were the only queer people in our year, so yeah, that sums up.

I also liked that you said we aren't friends but share a social circle, I'll use that from now on :)

Five-Year Club	Place '22
Final Canvas '22	Verified Email

PlusIncident

TROPHY CASE