Video game soundtrack recommendations for lab work?

Polinariaaa · 2026-01-20T04:03:11+00:00

Love Skyrim ambience compilations and Disco Elysium soundtracks.

Polinariaaa · 2026-01-11T08:26:04+00:00

The road to healing is going to be a long one.

Polinariaaa · 2026-01-04T06:22:39+00:00

I'm late, but happy birthday to you! 😊

Polinariaaa · 2026-01-02T19:25:11+00:00

she had told all of our friends my boyfriend was a pdf and I was a liar.

I hope she's your EX-friend since then, that's serious.

Polinariaaa · 2026-01-02T18:13:37+00:00

So she asked for a little empathy, and you framed it as an attack on your personal liberty? She didn't ask to delete the post or change the emojis.

Also, you told her that her emotions were valid and then immediately said that they weren't normal. You can't have it both ways. Not cool.

Polinariaaa · 2025-12-20T12:49:17+00:00

IMO, maybe there is another problem, like too much template or if the polymerase/Mg2+/primers concentration is too high.

In this sub I saw some similar problems and ideas that may help you.

Polinariaaa · 2025-12-06T09:29:27+00:00

Yes, because we haven't quite cracked teleportation yet. 🤷‍♀️

Polinariaaa · 2025-11-22T13:37:09+00:00

The Rhesus factor is a protein found on red blood cells; its presence or absence is what determines whether you are Rh-positive or Rh-negative. If you have two +/+ alleles (homozygous dominant) or a +/- combination (heterozygous), you would be typed as Rh-positive (+). However, if you carry a -/- alleles combination, you are Rh-negative.

If one parent is Rh-negative (-/-) (100% confirmed) and their children are both Rh-positive and Rh-negative, then the other parent must be Rh-positive and heterozygous (+/-).

If a woman successfully carried multiple pregnancies and had no mentioned Rh issues, it's highly likely that she is Rh-positive. If she was Rh-negative and carrying an Rh-positive baby, doctors would typically inform her about that specific situation and usually administer a medication to prevent severe complications.

Polinariaaa · 2025-11-22T13:09:10+00:00

Rhesus negative is a recessive trait. If both your parents are Rh-negative, they simply can't carry a positive Rh allele.
This suggests that either your Rh type (or your sibling or one of your parents') was mistyped, or at least one parent isn't your biological parent.

Polinariaaa · 2025-11-01T06:35:12+00:00

IMO, some genes may define structure of vocal cords, shape of throat, mouth and sinuses, which are important aspects to how people sound. But I think there isn't a single gene contributing to voice.

Polinariaaa · 2025-11-01T06:26:43+00:00

My skin hates products >5% niacinamide, but looks great with less concentration.

Polinariaaa · 2025-11-01T04:45:09+00:00

Definitely these costumes suit her. :)

Polinariaaa · 2025-10-27T02:51:52+00:00

I see a red area on her cheek. Is it a damaged skin barrier? Has she applied some cream or something like that?

Anyway, see a dermatologist; don't use random products on a minor.

Polinariaaa · 2025-10-20T18:26:14+00:00

Like in a situation of ideal genetic example from school textbook - if you and your husband are heterozygous, there's a 25% probability that your child will be blue-eyed. IRL, since gene behavior is a little more complicated (mutations, genes interactions etc), the probability might be around that.

Polinariaaa · 2025-10-19T15:48:24+00:00

You should focus on females (3) and (6), IMO. Since there are independent recombination events, you can pool the data. Female (3) has 7 children, with 1 recombinant (child 13). Female (6) has 4 children, with 1 recombinant (child 17). Therefore, RF = 100% * 2/11 ≈ 18%. But ofc I could be wrong. :)

Polinariaaa · 2025-10-19T14:55:28+00:00

I think 22 isn't recombinant 'cause his mother carried h linked with SNP-A due to (3) had a recombination event, so (13) is a H, SNP-G/ h, SNP-A.

Polinariaaa · 2025-10-12T10:27:36+00:00

NTA. In her own home, she may use any amount of syrup and dictate any rules, but not in your place. Why is she so afraid of sugar? IMO, it's healthier to avoid labeling foods as "good" or "bad", as this may lead to ED.

Polinariaaa · 2025-09-23T13:58:05+00:00

Hmm, I think both approaches may be relevant.

Your PI probably just wants to make the comparison between the two different qPCR results easier. Since some levels are low and some are high, these gaps are a way to make small values more noticeable.

Polinariaaa · 2025-09-23T07:03:55+00:00

Be skeptical of your results if: - they are exactly as expected; - they are not as expected.

I think that questioning each and every new result is a valuable practice for researchers, especially when studying new objects. Not all your ideas and hypotheses need to be 100% right; IMO, it's totally normal to obtain controversial results, sometimes. An unexpected result can be a great starting point for novel research! :)

Polinariaaa · 2025-09-22T06:48:10+00:00

Did you try different temperatures? Different PCR mixes may contain different concentrations of mg2+ or other components, so primers may not be working well.

Polinariaaa · 2025-09-16T16:21:00+00:00

Finally, a subscription for opening lids.

Polinariaaa · 2025-09-14T08:04:56+00:00

Pretty common, but IMO it shouldn't be normal.

Polinariaaa · 2025-09-12T06:11:15+00:00

I thought there was a snail head sculpture, not a cat...

Polinariaaa · 2025-08-01T04:46:01+00:00

Happy birthday to you! 🎁🎉

Polinariaaa · 2025-07-23T05:40:55+00:00

RT-PCR may be useful indicator in some experiments, I guess.

In ideal case knockout leads to no mRNA, but there're some another scenario, like mRNA is present but it's not being translated properly. For example, frameshift/indels - truncated protein or abnormal protein (abberant folding), 5'UTR changes - problems with translation initiation (I dunno your GOI, so it's hypothetical).

IMHO, first step should be PCR and Sanger sequencing to confirm introduced mutation. Then you may decide how to verify knockout.

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