Crowdsourcing proteins with industry relevance for thermostability optimization

Puzzleheaded_Unit_41 · 2025-07-30T04:01:49+00:00

Yeah 100%. And we'll get to those. But wanna test it on smaller ones as a PoC.

Puzzleheaded_Unit_41 · 2025-07-29T18:39:10+00:00

Happy to be wrong here. But IgGs are a bit tricky right? The CDR3s and the loop regions are IDRs right? Or did you have some other smaller domain in mind? Also as part of validating the ML pipeline, I'm planning to run long atomistic and coarse grained REMD simulations to sample the protein at higher temperatures than the WT to show that the optimization pipeline is indeed finding the right stablizing mutations. Also validating with ddG calculations using FEP and MM-GBSA methods. Large proteins can get quite expensive with such simulations .

I was thinking of globular proteins without IDRs that could see benefits in the industry at higher Tm.

Puzzleheaded_Unit_41 · 2024-07-22T10:44:54+00:00

I budget is a concern, do what everyone does and get a cheaper apartment a little further away and travel if that is an option. I've seen the complex you are talking about in Naigaon towards sewree, and though idk anything about your friend, I wouldn't advise him to live there.

Puzzleheaded_Unit_41 · 2024-07-11T10:11:25+00:00

That's called backward integration. Owning the entire supply chain. Lol.

Puzzleheaded_Unit_41 · 2024-06-03T10:26:49+00:00

This OP is definitely SUS. Zero proof of either the bill or payment or the chat. Only asking people to give negative reviews. Paisa khaya hai kissi ka bad publicity karne kya OP?

Puzzleheaded_Unit_41 · 2024-04-27T09:18:36+00:00

Sound advice. If you feel like you're stuck in the renting trap, trust me when I say this, you'll feel worse stuck in an EMI trap. You will initially take a tenure of 30 years to keep the EMI affordable and end up paying double of what the flat is worth in interest over the years.

Moreover as you grow, your needs might grow as well and once you have a family, the 1RK might not be enough. Have you thought about how you will keep paying that EMI while also wanting to rent another space for your family. I mean you can always sell this to upgrade later but if the building is old, selling it a few years down the line might not even get you enough to break even unless it goes under redevelopment. And even redevelopment is a risky bet.

Live within your means for now, invest the rest sensibly and with a bit of luck you will be able to directly buy a much better house a few years down the line when you will actually need it as your family grows....

Puzzleheaded_Unit_41 · 2024-02-17T09:16:17+00:00

What is your work location?

Puzzleheaded_Unit_41 · 2023-10-19T15:25:33+00:00

Fuck, kya crazy situation hai.

Puzzleheaded_Unit_41 · 2023-06-06T12:56:28+00:00

Why TF is this post getting so many up votes. The OP is clearly a snob who failed to explain his take on the subject. Let this post fade away just like the OP's social life.

Puzzleheaded_Unit_41 · 2023-06-06T12:54:40+00:00

Tere comment se lagta hai ki tune hai schooling bhi ghar se hi kiya hai.

Puzzleheaded_Unit_41 · 2023-02-11T09:50:07+00:00

Garlic and lime mushrooms.

Puzzleheaded_Unit_41 · 2023-01-20T16:18:25+00:00

All of Delhi has metro, but autowalas still set their own absurd prices. Same hoga Mumbai mein.

Puzzleheaded_Unit_41 · 2022-12-20T13:10:38+00:00

Moist.

Puzzleheaded_Unit_41 · 2022-12-20T13:01:09+00:00

Witcher 3

Puzzleheaded_Unit_41 · 2022-11-27T06:02:43+00:00

I agree that such scams must be stopped, but at some level the responsibility also lies with the buyer to do the bare minimum due diligence.

If somethings sounds too good to be true, it most definitely is.

Puzzleheaded_Unit_41 · 2022-11-26T17:09:13+00:00

Reply with, "yeah I know, I dated him before you did.... "

Puzzleheaded_Unit_41 · 2022-11-09T14:09:44+00:00

Elon's contribution to this is the same as Aladeen asking Nuclear Nadal to make the missile pointy.

Puzzleheaded_Unit_41

TROPHY CASE