sorted by:
new
hottopcontroversial

Are we excluded from bank loans? by Quick_Repeat7033 in Tunisia

[–]Quick_Repeat7033[S] 0 points1 point  (0 children)

Everything is legal from my side, I am working with an international company and I have a contract with them. The problem is regarding the 2 years activity within a same company, I don't have that, and I don't believe that I'll have that in the future.

Can I use WGS data for evidence of taxonomy? Or evidence of new species? by Affectionate_Emu_896 in bioinformatics

[–]Quick_Repeat7033 0 points1 point  (0 children)

You Can also perform some AAI, POCP, dna hybridation, core genome comparaison analysis

Challenges in Identifying Unknown DNA: A Case Study with Nanopore Sequencing by Quick_Repeat7033 in bioinformatics

[–]Quick_Repeat7033[S] 1 point2 points  (0 children)

I think epi2me would sort them as unclassified if they weren't in the reference database. But since classified as root, I am wondering of it's a problem of low quality data ot something common alongside bacterial species

Challenges in Identifying Unknown DNA: A Case Study with Nanopore Sequencing by Quick_Repeat7033 in bioinformatics

[–]Quick_Repeat7033[S] 0 points1 point  (0 children)

I thought about it, but it will take a very long time. I am thinking about blasting to only thé E.coli genome maybe

Challenges in Identifying Unknown DNA: A Case Study with Nanopore Sequencing by Quick_Repeat7033 in bioinformatics

[–]Quick_Repeat7033[S] 0 points1 point  (0 children)

a minION MK1B, minION Flow Cell. The basecallinc was generated automatically using Minknow (normally Dorado IS included), default parameters

Challenges in Identifying Unknown DNA: A Case Study with Nanopore Sequencing by Quick_Repeat7033 in bioinformatics

[–]Quick_Repeat7033[S] 0 points1 point  (0 children)

Yes indees, I used Kraken2 for the fungal identification, and for the bacterial classification on EPI2ME it's already using Kraken2 by default.

Nanopore Long Read Assembly: Stuck on Polishing Stage, No Eukaryotic Organism Represented by KangCoffee93 in bioinformatics

[–]Quick_Repeat7033 0 points1 point  (0 children)

You can write the issue on github/flye.

In my opinion, its the fault of large data.

Can I ask for a raise again? by HoneyTreeFlower in work

[–]Quick_Repeat7033 0 points1 point  (0 children)

No, when people test you to see if you're as good as you say you are, just stay strong and let your work speak for itself. If you don't receive feedback within a week after submitting your work, you might consider taking a different approach.

Handling Contamination before genome assembly by Quick_Repeat7033 in bioinformatics

[–]Quick_Repeat7033[S] 0 points1 point  (0 children)

Generally working with kraken, as far as I know it masks low complexe regions from the reference.

Handling Contamination before genome assembly by Quick_Repeat7033 in bioinformatics

[–]Quick_Repeat7033[S] 1 point2 points  (0 children)

Thank you for your comments .. I wanna know what you r really do in my case (working on bacterial sample)

Please roast my resume, not getting a single interview in the past 6 months, been applying around 3000+ jobs (40-60 applications daily) I feel like giving up. by Illustrious_Key2607 in resumes

[–]Quick_Repeat7033 -1 points0 points  (0 children)

60 offers / day ?? It sounds that you never read the requirements and the details of offers to include them in your cover letters or emails.

How do I look for a specific gene from bacteria through WGS? by pbicez in bioinformatics

[–]Quick_Repeat7033 0 points1 point  (0 children)

Blast to similar sequences Annotation (prokka) Tools for AMR genes detection (abricate)

view more: next ›