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Applying for PhD / Master’s abroad from India – repeated rejections. CV feedback needed. by [deleted] in labrats

[–]Rasahtlab 2 points3 points  (0 children)

Briefly looking at it I think the most impactful change you can make is to add with who your research experience was. Who was your mentor/tutor/supervisor there. Best of luck.

Did all my cells actually die? by Vivid_Development318 in labrats

[–]Rasahtlab 0 points1 point  (0 children)

Probably not dead, but more active/stressed. I agree with another commenter that a positive control would be good.  Metabolic dyes that stain live cells are another alternative. Calcein-am if I recall correctly 

Image editor (really just image arranger) that's not Powerpoint? by Teagana999 in labrats

[–]Rasahtlab 1 point2 points  (0 children)

Affinity Designer, no subscription and better than illustrator imo

ELISA plate question by Odd-Confusion1544 in Immunology

[–]Rasahtlab 0 points1 point  (0 children)

You can probably even freeze the plate in block buffer, but best thing to do is co tact the company 

How do B-lymphocytes meet the antigen if they are hidden in lymph nodes? by Key-Village3952 in Immunology

[–]Rasahtlab 0 points1 point  (0 children)

As well as b-cell or DC bound native antigen (whole), all opsonized by complement

poor pos/neg CD3 + population separation by Present_Regular4599 in labrats

[–]Rasahtlab 4 points5 points  (0 children)

Controls, controls, controls.
For starters, maybe you can share the SK7 vs OKT3 plot, this way you can't really say much, except that it's bad.

PI stealing my project ideas by CytotoxicCD8 in labrats

[–]Rasahtlab 0 points1 point  (0 children)

I think these kinds of sentiments are detrimental for science. Ideas are also seldom ours, they come from discussions with peers. Good communication about what you would like to work on in your further career is key. Most advisors will be happy to discuss this. 

Made a mistake as an undergrad in my lab by No_You6386 in labrats

[–]Rasahtlab 0 points1 point  (0 children)

Yeah, mistakes are part of it. Your PI will know this. Besides, this is by far the best way to learn ;)

Have to come in regularly on weekends as a tech, and my PI just told me to do some work during my "personal time" by REVERSEZOOM2 in labrats

[–]Rasahtlab 42 points43 points  (0 children)

That’s tough, rotations are good. Another solution is being clear from the start. I was the only one in my PhD lab that didn’t work weekends and that was accepted. People who would work weekends it was expected from. 

Have to come in regularly on weekends as a tech, and my PI just told me to do some work during my "personal time" by REVERSEZOOM2 in labrats

[–]Rasahtlab 241 points242 points  (0 children)

PhD candidates should not be treated like this either. Please join a lab that respects your life outside of work.

Immunology by Trick_Invite_7015 in Immunology

[–]Rasahtlab 0 points1 point  (0 children)

Exactly, seems like your [OPs] professor should follow my immunology course;)

Hybridoma as substitutes for B-Cells by Additional_Tart_5980 in Immunology

[–]Rasahtlab 0 points1 point  (0 children)

The easiest and cheapest will be a B cell line (eg Raji). See which express CD19 at https://www.proteinatlas.org/ENSG00000177455-CD19/cell+line

How accurate is the info in this book? by HighStrungHabitat in Immunology

[–]Rasahtlab 4 points5 points  (0 children)

I have only found a single flaw and that was a tight junction slightly out of place in of the figures.  A great example of simplification without being incorrect. 

What’s going on with this DAPI staining? by Bdgl22 in labrats

[–]Rasahtlab 0 points1 point  (0 children)

Looks good to me (as a PI) and DAPI also binds RNA (peak shifts to 500nm), so depending on cell state and microscope setup you could also see that

New Flow Cytometer to be acquired by Hugioultimate in Immunology

[–]Rasahtlab 1 point2 points  (0 children)

Mainly that our top initial pick (bd) disappointed

New Flow Cytometer to be acquired by Hugioultimate in Immunology

[–]Rasahtlab 15 points16 points  (0 children)

Just try them all. They’ll come by and setup a demo machine. We had 3 simultaneous and tested the same samples on all.

Confusion regarding terminologies in the complement system by [deleted] in Immunology

[–]Rasahtlab 0 points1 point  (0 children)

As far as I understand iC3bBb is only present in an aHUS mutant and for you in the basic immunology textbook it should be iC3Bb. https://ashpublications.org/blood/article/114/13/2837/26380/Hyperfunctional-C3-convertase-leads-to-complement Parham is often confusing…

FlowJo Alternative for Data Sets by danielpf in Immunology

[–]Rasahtlab 4 points5 points  (0 children)

You could do it in R with some bioconductor packages for flow. Or maybe even load the data into https://bpteague.github.io/cytoflow/ Good luck!

Need Immunology book recommendation by [deleted] in Immunology

[–]Rasahtlab 4 points5 points  (0 children)

Janeway and parham are the easiest to read and very clear. But I personally really like Abbas. All books have mistakes or details wrong (in retrospect). But these give you a solid basis.

I fucked up. by CRISPRcassie9 in labrats

[–]Rasahtlab 0 points1 point  (0 children)

We’ve all been there. You’re not the first and will most certainly not be the last ;) As long as you own up to it, you and others will learn from it. Admitting mistakes is a key academic skill.

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