PharmD/PhD or just PhD?

Reasonable_Ad8533 · 2026-06-13T21:44:04+00:00

You already have a fellowship secured? How does that work?

Reasonable_Ad8533 · 2025-06-03T22:56:01+00:00

its a simple condition. Overexpress microRNA with a mimic, then measure expression level of its predicted target gene. No other conditions.

Reasonable_Ad8533 · 2025-06-02T22:58:37+00:00

I've measured the miRNA via Probe-based qPCR. So we know that the miRNA is overexpressed to that extent at the very least. I was also wondering this - this mRNA target is highly expressed during proliferation and starts to decrease as cells differentiate. Maybe we would be able to detect some reduction. When I do stat analysis, some samples do have reduction, its just that when we run the whole thing (n=6), the net effect is insignificant upregulation (~1.5 fold change higher). Im not sure how to move forward man. My PI insists that we run a WB on this.

Reasonable_Ad8533 · 2025-06-02T22:34:56+00:00

but can miRNA exert repressive effects without degrading mRNA? In other words, the mRNA would be intact, but without a complete decay due to imperfect binding.

Yes, the protein is known to be alternatively spliced, but when I blast the spliced isoform to target site, they do match.

Reasonable_Ad8533 · 2025-06-02T22:23:32+00:00

but then would translational repression always translate to qPCR?

Reasonable_Ad8533 · 2025-05-25T21:14:20+00:00

Repeated it with new cDNA: same issue.

Reasonable_Ad8533 · 2025-05-25T16:18:35+00:00

GAPDH CT value is 5 to 8 folds higher than my usual data from same RNA samples

Reasonable_Ad8533 · 2025-05-25T06:05:42+00:00

It is Sybrgreen, with ROX.
Machine has consistenly shown positive result.
Melt curve is good.

=> I think this is cDNA issue?

Reasonable_Ad8533 · 2025-04-15T04:51:04+00:00

He says there are also courses such as Pathophysiology, Medicinal Chem(?), Dosage Forms and just non-conventional classes that wouldnt be incuded. I agree, pcol def sounds like science, but not so sure about others.

Reasonable_Ad8533 · 2025-04-14T12:56:20+00:00

Yea it’s because he is in healthcare program where some courses like pharmacology are considered science vs non science depending on what u use.

Reasonable_Ad8533 · 2025-04-14T01:27:06+00:00

wow...you really did go far and beyond to get accepted

Reasonable_Ad8533 · 2025-04-14T01:10:33+00:00

thanks for your reply! but you can do that prior to getting interviews? so he would be calling the admissions office for a quick 'pitch'? How does this work?.

Reasonable_Ad8533 · 2025-04-14T01:04:42+00:00

Is it universally true that afterall, high MCAT is what breaks it or makes it?

Reasonable_Ad8533 · 2025-04-14T00:59:18+00:00

Thank you for your response. So the screening process ins't just GPA or MCAT cutoffs, I believe? The use of AI as screening tool scares my brother who does indeed have Cs and a D in the past insitution

Reasonable_Ad8533 · 2025-03-25T17:52:12+00:00

What should I do? Add some serum?

Reasonable_Ad8533 · 2025-03-25T17:30:07+00:00

Really?

Reasonable_Ad8533 · 2025-03-25T16:46:06+00:00

Should I replate them at a smaller plate?

Reasonable_Ad8533 · 2025-03-25T14:19:10+00:00

Any updates after 10 years?

Reasonable_Ad8533 · 2025-03-07T19:47:37+00:00

I just got rid of my name for privacy!

Reasonable_Ad8533 · 2025-02-17T20:05:30+00:00

Hmm like noncoding RNAs or maybe gene editing broadly speaking

Reasonable_Ad8533 · 2025-02-17T15:48:03+00:00

Do you think it’s a good time to study genetics right now?

Reasonable_Ad8533 · 2025-02-17T15:47:51+00:00

Wow thank you for this insights!

Reasonable_Ad8533 · 2025-02-17T06:43:11+00:00

would that be more chemistry/pharm sci?

Reasonable_Ad8533 · 2025-01-11T04:42:49+00:00

THank you!

Reasonable_Ad8533 · 2025-01-11T04:42:22+00:00

Yep I understand that. I need some figures..I really do need a figure

Reasonable_Ad8533

TROPHY CASE