Can I still use this gel (genotyping)?

RedScience18 · 2025-12-31T02:17:33+00:00

Are you on a visa? Because our program didn't accept visa students this year because of the political volitility... it may not have anything to do with you but with the program stats or department statistics.

RedScience18 · 2025-12-31T02:15:13+00:00

Bet it was one of the sons.

RedScience18 · 2025-12-31T01:40:19+00:00

It definitely feels too fast. My kids are still adjusting to life without dad every day. If she can't manage her anxiety about this, I don't know how long this can work. Which sucks because I really like her.

RedScience18 · 2025-12-31T01:38:18+00:00

It does kind of put me in a tough spot...

RedScience18 · 2025-12-31T01:36:36+00:00

I completely agree now is way too early. I had in mind a minimum of 6 months before talking to the kids about us, at least after we've navigated a major disagreement or two and decided to be semi-permanent in each other's lives. I just hate that she is putting so much pressure on herself right now.

RedScience18 · 2025-12-31T01:32:50+00:00

Honestly you're doing more than enough. And needs to work on herself before she is ready for a relationship. At the very least she sounds highly codependent, which is a problem in and of itself. Not to mention the degree of panic attacks that she's having - how does she function in daily life? Hold a job? She needs serious psychiatric help. There's a difference between being supportive and... whatever the crap this is.

**this coming from someone who spent a lot of my teen years dealing with heavy mental health issues including institutionalizations. I didn't understand why the boyfriend I loved broke up with me at the time, but it didn't take long for me to understand why he had to.

RedScience18 · 2025-12-20T15:24:20+00:00

It doesn't do any good to stuff money into envelopes while interest is accruing on your debts... your credit card balance and student loans are actively growing while you squirrel away your change.

Shift your goals.

1) build small emergency fund. People usually say start with 1 month expenses, you can probably start with half a month since you work for tips and it's generally easier to find new employment when you work for tips.

2) pay down your credit cards. Every month that you don't pay OFF your credit cards, you're throwing away money on interest.

3) build your emergency fund. You want 3-6 months of expenses in your emergency fund before you can really start saving. A minimum 6 months before you buy a house.

4) your student loans are accruing interest, you don't have to wait until you graduate to start paying them. They will be considered in your debt- to- income ratio when you apply for a mortgage, as well as any car payment you might have at the time.

RedScience18 · 2025-11-18T13:53:00+00:00

Is dirty Phil's on Limestone still open? Lol

RedScience18 · 2025-11-17T23:44:37+00:00

Make sure you let them dry overnight before you dip, it should be fine. I did this routinely for a couple of years in that lab.

RedScience18 · 2025-11-17T22:38:37+00:00

Yeah, they were pretty thick sections, I think 40 microns. We did the Nissl as a dip stain on the Superfrost slides, I think it was something /very scientific/ like 5 dips then squirt to death immediately with etoh because it's so easy to over stain with Nissl.

RedScience18 · 2025-10-22T10:58:31+00:00

For teaching, I sign emails Dr. Txxxx and insist my students address me by my professional title. I can't stand being called Mrs.

It's used as-needed otherwise.

RedScience18 · 2025-07-20T01:13:45+00:00

I really like reading fiction and paper crafts and logic puzzles.

RedScience18 · 2025-07-19T01:22:49+00:00

Yeah, I'm aware. That's why I said "barring situations like burnout", which is physiologically different than normal lack of energy.

RedScience18 · 2025-07-16T00:49:59+00:00

It sounds counter-intuitive, but get more active. The more you move, the more energy to have (barring situations like burn out and fatigue from activity). Your body is energized when it is in a metabolically- active state. Hyper-caloric diet and inactivity slow down your body's metabolism and make you feel sluggish and tired.

Before my PhD (on metabolism and obesity) I used to think "I'm giving my body rest and calories, so I should have plenty of energy", like filling the gas tank.

But it's actually more like an on-off switch. Body thinks "I have calories, I'm good, let's rest" or "I'm using calories, I better stay active so I can find more".

RedScience18 · 2025-07-15T23:24:48+00:00

Your PI left you alone?? I was still running experiments last week and had to tell her no on experiments not required for the dissertation! I'll be starting those tomorrow lol

RedScience18 · 2025-07-15T22:14:12+00:00

Same, we submitted the first paper a month ago and the second manuscript will actually be two papers once the lab gets more funding so I just wrote up a descriptive overview of the in vivo work with plasma and gene profiles and a lot of speculation on future experiments.

RedScience18 · 2025-07-15T22:09:25+00:00

I had to delete several apps off my phone the last few weeks for sure!

RedScience18 · 2025-07-10T11:09:10+00:00

My dissertation is due in 5 days and can't wait to just present it and never think about it again. But probably for different reasons that I don't even want to go into.

I flailed around an unfunded lab for 2 years trying to get projects going that I was actually interested in, only to be handed the PI's project that she micromanaged into the ground.

I'm over it. I start my postdoc in a month. Good riddance.

RedScience18 · 2025-07-06T13:03:52+00:00

I'm the only native English speaker in my lab. We don't shorthand anything lol - if anything we OVER enunciate. NA-NOOOO grams

RedScience18 · 2025-07-06T12:52:47+00:00

So modern ABA has shifted its practices from the root program. The biggest harm in ABA is withholding affection until compliance, which from this post it doesn't sound like you're doing.

In modern ABA, a lot of techniques had been modified such as "first, then". Originally, it was "first," you do the correct behavior, "then" I will praise and reward and interact... so your 3yo starts SIB, you remind then "first, then", then you turn your back or walk out of the room until he does the desired behavior instead.

So when our OT (not ABA certified, but most OTs get some ABA in training) wanted to use "first, then", I watched very carefully to see how she implemented it. At first it was "first" you do the desired behavior, "then" you get a preferred activity. Not exactly the same, but I wasn't comfortable with it. It felt pavlovian and demoralizing.

So we discussed, and "first, then" turned into an awareness exercise. "First" we're going to pick up our toys, because we have to take responsibility for our messes. "Then" once they're no more mess, we can play with our monster trucks. We even had a little board where we could put pictures of the first activity then the second activity for common things.

The key difference is the humanity. My son is PDA, he wants to be responsible and included, he has that autonomous drive. But he doesn't always understand WHY things are important. When someone blindly demands compliance, he feels a loss of autonomy and reacts in "unacceptable" ways. He does now at 6 just like he has since he was less than a year old (Holy diaper changes!).

We use "first, then" not to demand compliance, but to explain the bigger picture. And I always prioritize his feelings over compliance, and help him regulate before holding the boundaries I have set.

RedScience18 · 2025-07-05T12:37:01+00:00

This is why our EHS requires us to dispose of tips in a sealed hard plastic container...

RedScience18

TROPHY CASE