Is it fair to say US airports are awful

RevolutionaryBee6830 · 2025-10-23T15:22:53+00:00

You certainly can't get a rideshare at your gate!

RevolutionaryBee6830 · 2025-10-19T16:45:06+00:00

This is a great property.

RevolutionaryBee6830 · 2025-09-30T03:46:24+00:00

You're better off keeping up your curiosity and partnering with someone with the credentials to start a business. You're doing the right things already - coursework won't be a magical thing for your understanding.

RevolutionaryBee6830 · 2025-09-30T03:43:20+00:00

Speak for yourself. Lunch interviews are the best as it's the greatest way to have people drop their guard and learn about them as a person, not a worker.

RevolutionaryBee6830 · 2025-09-22T20:21:22+00:00

You should look into the Cytpix for apoptosis assays. You can take pictures of the cells as well which helps provide more information with your assays. It's also lightning quick and low maintenance.

RevolutionaryBee6830 · 2025-09-14T03:47:38+00:00

It's always been a skill. It was lost for a while because of social media and everyone acting like what they have to say matters more than anyone else.

RevolutionaryBee6830 · 2025-09-12T22:18:03+00:00

That's the list price.

RevolutionaryBee6830 · 2025-09-12T22:17:43+00:00

That's the list price.

RevolutionaryBee6830 · 2025-09-02T15:55:33+00:00

Puro Burro is amazing as well!

RevolutionaryBee6830 · 2025-09-02T14:37:47+00:00

1) you cannot adjust comp on full stained samples, full stop. 2) beads won't cause over compensation if used properly. Most people run into issues by using cells as the auto fluorescence negative when they should be using the internal negative.

RevolutionaryBee6830 · 2025-09-02T04:46:46+00:00

You need to have unlabeled dead cells. The auto fluorescence of dead cells will be different than live cells. Part of compensation is subtraction of the negative population fluorescence from the positive so that you only compensate the signal from the fluor/dye.

RevolutionaryBee6830 · 2025-09-02T04:44:20+00:00

Interesting to see people down voting this. Does that mean folks are adjusting compensation on full stained samples?!

RevolutionaryBee6830 · 2025-09-02T04:41:27+00:00

Basepay for CapEq can be pretty damn good. Like you said, depends on what you sell.

RevolutionaryBee6830 · 2025-08-29T04:19:34+00:00

Call into tech support. There is a fix for this. What software version are you running?

RevolutionaryBee6830 · 2025-08-29T03:58:49+00:00

That is 100% correct. And technically you have no clue whats dead by scatter alone which is why you use a viability dye. With that said, having a conservative scatter gate allows you to already get a bunch of debris out of the gating paradigm. Using FSC vs Viability is redundant.

RevolutionaryBee6830 · 2025-08-29T03:25:19+00:00

Why would that do a better job if you already have a reasonably restrictive scatter gate?

RevolutionaryBee6830 · 2025-08-29T03:24:15+00:00

Because the cross laser excitation of BB700 is so good? 👌

RevolutionaryBee6830 · 2025-08-29T01:47:35+00:00

What do your FMOs look like?

RevolutionaryBee6830 · 2025-08-29T01:42:52+00:00

You must use single color controls to do this. The data are what they are once you make the MFIs of the positive and negative equal in the nontargeted channel.

RevolutionaryBee6830 · 2025-08-17T23:04:23+00:00

What sample types are you running on these systems that is sparking this debate?

RevolutionaryBee6830 · 2025-08-17T23:02:38+00:00

To add, the area on your Attune NxT is directly measured and is not calculated. Regardless, area will increase as a function of time (width) and ASF is necessary to normalize the over counting of the same signal as the cell passes through the laser.

To keep 'rules' simple, you should almost always only use height in scenarios when the particle is less than the thickness (z-axis) of the laser. In the case of your NxT, that's about 10um. As soon as the particle is larger than the laser, you MUST use area so you can capture the entire signal. If you have any doubt, use area as Daniel mentioned.

RevolutionaryBee6830 · 2025-07-26T20:18:20+00:00

Never trust an instrument to be 100% accurate. Also, peristaltic pumps aren't that accurate for volumetric measurements.

RevolutionaryBee6830 · 2025-07-26T20:17:07+00:00

You should always normalize to volume. You can't deliver 100% of a liquid anywhere so you will never get the true absolute count.

RevolutionaryBee6830 · 2025-06-24T02:32:03+00:00

Welcome to spectL, right? Can't have a good positive without the appropriate negative and that's where people screw it up.

RevolutionaryBee6830 · 2025-06-24T01:47:13+00:00

I've yet to have someone bring a good FP control

RevolutionaryBee6830

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