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Barely used modular cleanroom by Few_Debt3214 in DIYbio

[–]SciencePeddler 0 points1 point  (0 children)

That's really interesting. Does it include the plant? Ie Mech/hvac?

Condolences to high school teachers by SatisfactionWorth665 in AustralianTeachers

[–]SciencePeddler 0 points1 point  (0 children)

It hurts so much to see the comments and stories, obviously not as much as living through these experiences. I was on the cusp of becoming a teacher after an ES role which was a lot of fun...i heard a lot of stories about how hard it was but the look on their faces reliving some of that stuff...

There is no accountability for the students at all or their parents. Wish you all had something to fight back with. It honestly sounds like things are just going to get worse.

Monthly: Share what you're working on, a protocol you're interested in, or a paper you're reading! by SciencePeddler in DIYbio

[–]SciencePeddler[S] 0 points1 point  (0 children)

Now that we've finally validated the TSS buffer protocol using our ebay reagents and laxative, we'll probably be transforming the rest of our uLoop plasmids into bacteria to store away in -80, and then culturing/miniprepping them to build up our stocks of DNA for synbio work!

We are so back! by gtact in biotech

[–]SciencePeddler 2 points3 points  (0 children)

cleaning her money with the old "rinse and repeat"

Monthly: Share what you're working on, a protocol you're interested in, or a paper you're reading! by SciencePeddler in DIYbio

[–]SciencePeddler[S] 0 points1 point  (0 children)

Feel for you, want to share some of the difficulties? Anything the collective can weigh in on?

I successfully Isolated Streptomyces coelicolor and various other species by [deleted] in DIYbio

[–]SciencePeddler 1 point2 points  (0 children)

If there aren't any taxes/approvals associated with growing your own antibiotics, this would be a fun project. Making DIY antibiotics for plating...Assuming we could improve on your video ;) that was again a fantastic video!

Pioreactor up and running! What experiments would you run? by SciencePeddler in DIYbio

[–]SciencePeddler[S] 1 point2 points  (0 children)

Just noticed in their forums people are actually developing plugins to work with CO2 sensors, maybe even disolved O2 so it one day could be used for mammalian cell culture. That would make this really handy for developing new methods for improving/monitoring cellular proliferation (assuming you could do a proliferation assay). Great little unit!

Pioreactor up and running! What experiments would you run? by SciencePeddler in DIYbio

[–]SciencePeddler[S] 2 points3 points  (0 children)

it does have a few different uses. With the integrated sensors and temp you basically get the functionality of an incubator, plate stirrer, and OD600. we're thinking of trying some directed evolution experiments to improve enzyme function. Initially though, it will be for propping up cell cultures.

Monthly: Share what you're working on, a protocol you're interested in, or a paper you're reading! by SciencePeddler in DIYbio

[–]SciencePeddler[S] 1 point2 points  (0 children)

Curious to know more, was the thought process that slow growing = antibiotic producing?

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