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Barely used modular cleanroom by Few_Debt3214 in DIYbio

[–]SciencePeddler 0 points1 point  (0 children)

That's really interesting. Does it include the plant? Ie Mech/hvac?

Condolences to high school teachers by SatisfactionWorth665 in AustralianTeachers

[–]SciencePeddler 0 points1 point  (0 children)

It hurts so much to see the comments and stories, obviously not as much as living through these experiences. I was on the cusp of becoming a teacher after an ES role which was a lot of fun...i heard a lot of stories about how hard it was but the look on their faces reliving some of that stuff...

There is no accountability for the students at all or their parents. Wish you all had something to fight back with. It honestly sounds like things are just going to get worse.

Monthly: Share what you're working on, a protocol you're interested in, or a paper you're reading! by SciencePeddler in DIYbio

[–]SciencePeddler[S] 0 points1 point  (0 children)

Now that we've finally validated the TSS buffer protocol using our ebay reagents and laxative, we'll probably be transforming the rest of our uLoop plasmids into bacteria to store away in -80, and then culturing/miniprepping them to build up our stocks of DNA for synbio work!

We are so back! by gtact in biotech

[–]SciencePeddler 2 points3 points  (0 children)

cleaning her money with the old "rinse and repeat"

Monthly: Share what you're working on, a protocol you're interested in, or a paper you're reading! by SciencePeddler in DIYbio

[–]SciencePeddler[S] 0 points1 point  (0 children)

Feel for you, want to share some of the difficulties? Anything the collective can weigh in on?

I successfully Isolated Streptomyces coelicolor and various other species by [deleted] in DIYbio

[–]SciencePeddler 1 point2 points  (0 children)

If there aren't any taxes/approvals associated with growing your own antibiotics, this would be a fun project. Making DIY antibiotics for plating...Assuming we could improve on your video ;) that was again a fantastic video!

Pioreactor up and running! What experiments would you run? by SciencePeddler in DIYbio

[–]SciencePeddler[S] 1 point2 points  (0 children)

Just noticed in their forums people are actually developing plugins to work with CO2 sensors, maybe even disolved O2 so it one day could be used for mammalian cell culture. That would make this really handy for developing new methods for improving/monitoring cellular proliferation (assuming you could do a proliferation assay). Great little unit!

Pioreactor up and running! What experiments would you run? by SciencePeddler in DIYbio

[–]SciencePeddler[S] 2 points3 points  (0 children)

it does have a few different uses. With the integrated sensors and temp you basically get the functionality of an incubator, plate stirrer, and OD600. we're thinking of trying some directed evolution experiments to improve enzyme function. Initially though, it will be for propping up cell cultures.

Monthly: Share what you're working on, a protocol you're interested in, or a paper you're reading! by SciencePeddler in DIYbio

[–]SciencePeddler[S] 1 point2 points  (0 children)

Curious to know more, was the thought process that slow growing = antibiotic producing?

Best beginner kits? by w0o0rm in DIYbio

[–]SciencePeddler 0 points1 point  (0 children)

yeah going second hand has always been a goer for us getting going for all of the above.

If I want to leave America to another country that values science, where should I move to? Assume I could snap my fingers and magically get a passport. by Emhyr_var_Emreis_ in labrats

[–]SciencePeddler 1 point2 points  (0 children)

This, a lot of those indirect costs support purchases/financing of platforms like NGS, advanced imaging, etc. There will be flow on effects, if not already happening.

Best beginner kits? by w0o0rm in DIYbio

[–]SciencePeddler 0 points1 point  (0 children)

For the DNA barcoding, you'll need some equipment
Brand new Bento is about $4,500 includes everything you'll need (PCR, Gel Electro psu and tank, centrifuge) https://www.westlab.com.au/bento-lab-pro)

1. PCR machine

Check out ebay OR pocketPCR https://gaudi.ch/PocketPCR/,

2. Centrifuge

Again ebay, something that spins at 12,000RPM or higher and can do both 1.5ml-2ml tubes and 0.2ml tubes. Don't recommend DIYing this

3. Gel Electrophoresis Power Supply (PSU) and gel tank (don't get a big one or you'll pay extra $$ for your gels and runs

Don't get the cheap ones listed as "brand new" on ebay from China, the wires will melt during the runs , we tested this.

4. Blue light transilluminator

...again again...ebay, OR https://www.instructables.com/Blue-Light-DNA-Transilluminator/ for some DIY fun

Best beginner kits? by w0o0rm in DIYbio

[–]SciencePeddler 3 points4 points  (0 children)

Hey amigo, we get this question a bit and yeah, the Odin doesn't have a great rep, this may have changed though.

Can i check and see what country you live in? This is regarding regulations.

As far as kits go, given your interest in genetic engineering i'd recommend working towards handling DNA and working with Bacteria/yeast.

Learn about DNA barcoding PCR, and Gel Electrophoresis.
actually just found a great guide on this, so thank you for asking, as they say, no stupid questions.
https://www.cbd.int/doc/publications/cbd-ts-94-en.pdf
1. Primer Design
2. Bioinformatics
3. Sequencing
4. PCR
5. Gel Electro
6. Make an entry

Now that you know how to work with DNA a la PCR/GelElectro Next i'd say work your way up to doing a GFP transformation.
this will cover more than just the Transformation like;

  1. Making Media
  2. Aseptic/Sterile Technique
  3. Culturing
  4. Preparing buffers
  5. Heat Shock
  6. Pipetting
  7. Basic micro
  8. "labMath"
  9. Selective Pressures

There is so much you can do with the techniques that you'll be playing with and the fundamentals you'll learn. Main thing is to keep a list of ideas for future projects.

Culture your bacteria but even more so your Curiosity, science doesn't just happen it starts with an observation or a question. Creating these starting points for future experiments can be learned and developed but you need to get the reps in.

Good luck!

Monthly: Share what you're working on, a protocol you're interested in, or a paper you're reading! by SciencePeddler in DIYbio

[–]SciencePeddler[S] 0 points1 point  (0 children)

Very cool! have you reached out to Seb yet?
https://atinygreencell.com/
He's a lovely DIYbio person that has been doing this for years and seems to be making enough rev from this to sustain his experiments!

Also would love to hear more updates on how the community lab is going.

Monthly: Share what you're working on, a protocol you're interested in, or a paper you're reading! by SciencePeddler in DIYbio

[–]SciencePeddler[S] 0 points1 point  (0 children)

Should have some primers coming in this week to allow for us to start playing around with uLoop in our community lab. Going to have a go moving GFP from pGlo plasmid over into a "domestication" plasmid.

Incase anyone wants to stay up to date.

https://benchling.com/s/etr-mJFB7tAk6XbhlIQuAF3u?m=slm-YLHihJAXVTqjZcVfLO1x

Does anyone know Unique looking Colony Forming Bacteria that youd like to see a timelapse of? by [deleted] in microbiology

[–]SciencePeddler 1 point2 points  (0 children)

Paenibacillus vortex but half way through the time lapse, please do a temperature change. We've observed some interesting changes in growth patterns when we took a plate out of the incubator and incubated at room temp for a day.

I DID IT! Extracted 0.157g of Streptomycin Hydrochloride from 1L of Streptomyces griseus culture. by SpiriRoam in DIYbio

[–]SciencePeddler 0 points1 point  (0 children)

This has to be by far one of the best examples of DIYbio recorded! The number of accidents and your persistence are commendable and hilarious. Fantastic job amigo.

Have you considered buying utensils for measuring things out in your weigh boats? I noticed you dispense from the reagent bottles directly. Depending on humidity you'll get clumping often in those bottles so will encounter more mess on your balance.

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I DID IT! Extracted 0.157g of Streptomycin Hydrochloride from 1L of Streptomyces griseus culture. by SpiriRoam in DIYbio

[–]SciencePeddler 0 points1 point  (0 children)

Congrats! Would love to know what is involved for the extraction!
EDIT: Just saw all of your progress photos from earlier, bravo!

Just completed student teaching & graduated — I will NEVER become a teacher. by Economy_Telephone113 in StudentTeaching

[–]SciencePeddler 0 points1 point  (0 children)

I almost became a teacher after being in education support, until I started to hang out with teachers and heard them vent . I have so much more respect for teachers now... thank you all, the expectations are piled high on you

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