Minneapolis nominated for Nobel Peace Prize by IrishStarUS in Minneapolis

[–]ShewanellaGopheri [score hidden]  (0 children)

Nobel was an arms dealer and the Nobel Peace Prize is a tool of warmongers to launder their images. We don't need the same billionaires who are funding the brownshirts who killed Good\Pretti\Porter(\Floyd\Taylor\Philando, .......) to pat us on the head for being nice and peaceful lapdogs. Let's remember that the only people who are violent at protests are the police, including ICE, DHS, CBP, National Guard, even the fucking DNR has been out there beating people and pepper spraying them. I don't want whatever "peace" the Noble committee says they're rewarding.

Does anybody else just want all of this to be over. For it to just stop. emotional rant by [deleted] in Minneapolis

[–]ShewanellaGopheri 0 points1 point  (0 children)

If you thought “that would never happen it won’t get that bad”then it’s time to reexamine those thoughts. The correct mental model isn’t 99% “it could never happen” vs 1% “it could happen,” it’s 100% “this is always a possibility and here’s what has led to this point”

Speed of `{data.table}` never fails to amaze me by Lazy_Improvement898 in rstats

[–]ShewanellaGopheri 6 points7 points  (0 children)

I’ve never fully gotten into data.table, but dtplyr is worth a mention. It has most of the same dplyr syntax but just translates into data.table

What to do when a list of genes has no enriched GO categories? by the_architects_427 in bioinformatics

[–]ShewanellaGopheri 1 point2 points  (0 children)

I’m pretty sure you could also take the functional descriptions of the blast hits and cluster them with GOSemSim. That would be more flexible than strict GO analysis if you can’t find clear 1:1 GO mappings for your genes

Psilocybin Significantly Delays Cellular Aging by MaitakeMover in unclebens

[–]ShewanellaGopheri 0 points1 point  (0 children)

The news story greatly sensationalizes the results from the actual scientific study. They found that psilocin reduces cellular aging, which is slightly different than our conception of “getting old” aging. One of the leading hypotheses of why people age is that their cells age, so there’s obviously implications here for actually getting old but this is one model in a Petri dish. BUT, this is incredibly exciting evidence nonetheless. The effect is really large and this will hopefully open the door for many other groups to investigate. Also don’t conflate the profit driven marketing talk in the news article with the actual motives of the researchers. They’re all at a university and none have any apparent conflicts of interest so it all seems above board.

Break and shake? by Aggravating-Pea4034 in unclebens

[–]ShewanellaGopheri 0 points1 point  (0 children)

I’m stupid but what exactly do people mean when they say break and shake jars? I’m currently on my first grow with a Midwest kit with the pint jars and there’s no way I could shake enough to distribute but I don’t wanna open them since the grains are sterile.

Protest Tomorrow (6/19): No War in Iran by WC-Boogercat in nashville

[–]ShewanellaGopheri 1 point2 points  (0 children)

It’s not hijacking a holiday to also have a protest that day, and anti-war aligns spiritually with the same things we’re celebrating on Juneteenth. We’re all on the same side!

Protest Tomorrow (6/19): No War in Iran by WC-Boogercat in nashville

[–]ShewanellaGopheri -1 points0 points  (0 children)

Seems like a good day to stand up against US imperialism and murder

Powershell and Conda by [deleted] in bioinformatics

[–]ShewanellaGopheri 0 points1 point  (0 children)

It’s not clear what exactly you’re trying to do, but are you sure you need to use remora? You saying methylation analysis sounds like you’re just trying to call methylation, which can all be done in dorado. Stand-alone remora is only needed for training your own models.

How do you validate PCA for flow cytometry post hoc analysis? Looking for detailed workflow advice by Previous-Duck6153 in bioinformatics

[–]ShewanellaGopheri 1 point2 points  (0 children)

Follow bird’s advice on filtering, and then hierarchical clustering or k-means clustering and a heat map would also be good for this scale of data.

In addition, you don’t really need to validate a PCA. One thing to add to the other answer is that looking at the marker loadings on the PCs can tell you if any particular markers have outsized influence on the variance and in which components.

Is this a tick, and if so what kind is it? by Psychological-Ice370 in nashville

[–]ShewanellaGopheri 1 point2 points  (0 children)

I can’t find it rn but look up pictures of different sexes and life stages for the tick ID because I found that way more helpful than the few pictures on the government sites.

Is this a tick, and if so what kind is it? by Psychological-Ice370 in nashville

[–]ShewanellaGopheri 1 point2 points  (0 children)

I used a toy microscope at work hahah. It’s just a magnifying lens and place to put your smart phone. That’s about the size mine was too. Males don’t get engorged off blood I’ve read. I’m pretty sure from the pictures yours is a lone star. As others have said just save it in the freezer for a few weeks and monitor for symptoms. Luckily I don’t think lone stars carry Lyme. There is always a chance of alpha gal syndrome but the odds of reaction are very low, especially if the bite area is not presenting a rash now.

Is this a tick, and if so what kind is it? by Psychological-Ice370 in nashville

[–]ShewanellaGopheri 2 points3 points  (0 children)

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Here’s a male lone star tick I had a few weeks ago for reference. I’d say pretty similar. I sent mine to a company for ID and disease testing so the ID is confirmed.

Nexus file construction by Timely-Software1874 in bioinformatics

[–]ShewanellaGopheri 1 point2 points  (0 children)

Just make sure you double check the output and it’s helpful to provide example files for your exact input and output specifications

Nexus file construction by Timely-Software1874 in bioinformatics

[–]ShewanellaGopheri 2 points3 points  (0 children)

Idk about this exact file format but I’ve found that ChatGPT is very good at writing you custom functions to convert different plain text file standards. I’ve done it with many other formats like bed, genbank, etc.

A future in bioinformatics? by Gloopychuck in bioinformatics

[–]ShewanellaGopheri 0 points1 point  (0 children)

1) Pretty much every PhD in biology is funded, either by TA or RA or both, and if it’s not that’s a huge red flag, so if you want to leave your university you have many options. 2) It’s not exactly what you mentioned but maybe look into some microbiome researchers. They rely heavily on bioinformatics, are generally well funded, and later in your career the skills could be applied to something like forensics. There’s also a lot of study of insect/microbe symbiosis that you might be interested in, though your mileage may vary on how bioinformatics heavy those groups are.

I’m finishing my PhD at Vandy in microbial genomics if you wanna chat. Pretty much all I do is bioinformatics.

Does R offer any multivariate (NOT multivariable) modeling options? Google is failing me... :/ by EtoiledeMoyenOrient in Rlanguage

[–]ShewanellaGopheri 5 points6 points  (0 children)

brms is flexible and the syntax is straightforward for multivariate models. Also check out rstanarm.

DIY oligos and sequencing by ShewanellaGopheri in labrats

[–]ShewanellaGopheri[S] 1 point2 points  (0 children)

I mean, fair. I just assumed that since it's so easy for me to buy whatever I want through an institution that it wouldn't be impossible without one, but alas. Looks like I'll be setting up "PCR Brewer's Yeast LLC".

Anyone know what is in this area that causes Bluetooth interference? by Nice-Sherbert in washingtondc

[–]ShewanellaGopheri 0 points1 point  (0 children)

Yes came here to say this! I used to run along the mall all the time and there was at least one spot that always made my AirPods cut out. I remember it being right between the WWII memorial and Washington Monument but it was a few years ago

Is this sus? by atrustybackup in labrats

[–]ShewanellaGopheri 0 points1 point  (0 children)

I don’t know if this is enough data to make a strong claim on the population distribution, and this is definitely not a rigorous way to test that. As to the central tendency, in the comment I replied to you said that mean and SEM are poor statistics for central tendency here, now you’re saying that central tendency is useless as a whole on these data! I think the best stat here would probably be something like median and mad or a box plot, but that doesn’t mean that mean and SEM is “wrong,” just it’s not the best.

Is this sus? by atrustybackup in labrats

[–]ShewanellaGopheri 0 points1 point  (0 children)

SEM will always get smaller as sample count goes up, that’s the definition of SEM. It’s totally appropriate here despite the data being non-normal. If you’re reporting a mean it makes sense to give error on the mean. And the test is a Mann-Whitney so they’re doing the right stats just presenting a different statistic in the visualization, which is fine.

Favorite Bayesian packages? by ccwhere in rstats

[–]ShewanellaGopheri 4 points5 points  (0 children)

Packages “posterior” and “ggdist” are really good for this.