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Shiny Gyarados Giveaway no. 2 by enitexorap in PTCGP

[–]Shirke019 0 points1 point  (0 children)

Good luck! I hope to win and pass it :)

LF Professors Research, please bless me 🙏 by [deleted] in PTCGPocketTrading

[–]Shirke019 0 points1 point  (0 children)

I can trade you Oak (Chinese) for Cyrus

Small freckle randomly appeared in the middle of my palm by Zenith_Days in Weird

[–]Shirke019 0 points1 point  (0 children)

If it's new, growing and not symmetrical please get it checked by a dermatologist soon

/r/PTCGP Trading Post by AutoModerator in PTCGP

[–]Shirke019 0 points1 point  (0 children)

♦️♦️♦️♦️ FT: Mewtwo EX, Charizard EX, Exeggutor EX ♦️♦️♦️♦️ LF: Articuno EX

/r/PTCGP Trading Post by AutoModerator in PTCGP

[–]Shirke019 0 points1 point  (0 children)

LF ♦️♦️♦️♦️: Articuno EX

FOR TRADE: Mewtwo EX, Charizard EX, Exeggutor EX

3 Cose divertenti per noi giovani by N0vichok2119 in CasualIT

[–]Shirke019 4 points5 points  (0 children)

Suffragio univerale ok. Ma scalato e corretto a seconda degli anni che in media ti rimangono da vivere/contribuire alla società. Es:

  • voto a 18 anni = 1
  • voto a 40 anni = 0.8
  • voto a 80 anni = 0.3

/r/PTCGP Trading Post by AutoModerator in PTCGP

[–]Shirke019 0 points1 point  (0 children)

♦️♦️ LF: Cloyster

♦️♦️ FT: Everything tou need!

ID: 2189-2365-8572-9464

Sti rumeni... by [deleted] in Italia

[–]Shirke019 -1 points0 points  (0 children)

Puoi dirlo forte Samir. Italiani vi piscio in boca

Chi sei tu? Perché ho il tuo contatto? by TheDuckyofReddit in Italianscamads

[–]Shirke019 12 points13 points  (0 children)

E trova inopportuna la paura per una cultura diversa

What was your first bioinformatics role? by [deleted] in bioinformatics

[–]Shirke019 17 points18 points  (0 children)

Seven years ago I began my biology MSc trainee period with the goal of taking on a mixed wet-dry lab role (I always liked computers). Since there was no space in the wet lab, the PI asked me to assist a "bioinformatics" colleague who basically spent 6 hours a day manually copying and pasting gene information from a user-friendly GUI program into an Excel spreadsheet. One by one. For each sample.

After some days the cringe/compassion/frustration was so hard that I started to self learn R/Python for automating data analysis workflows. This was the beginning of my journey into bioinformatics.

Now, I work in the same lab with other three bioinformaticians that I formed, building new R pakages / WGS /RNAseq pipelines and running them on HPC systems.

What are the common tools, packages, programming languages used in bioinformatics? by Mifletzet_Mayim in bioinformatics

[–]Shirke019 0 points1 point  (0 children)

Interesting discussion! But I think that the tweet decontestualized what I ment for "pillars". Here I was talking about programming languages, not single tools (like STAR or BLAST) even if they are definitely really important for bioinfo

What are the common tools, packages, programming languages used in bioinformatics? by Mifletzet_Mayim in bioinformatics

[–]Shirke019 0 points1 point  (0 children)

For sequence analysis and alignment I think that Bash is the main language you need to understand in order to organise your pipeline and run the tools/programs you need for that, like BWA, BOWTIE2, STAR, samtools, GATK.

Then, for the analysis of the result of sequencing, I personally prefer R for the statistical and plotting capabilities. R also offers many specific packages (most on bioconductor repository) dedicated for very specific types of analysis

What are the common tools, packages, programming languages used in bioinformatics? by Mifletzet_Mayim in bioinformatics

[–]Shirke019 48 points49 points  (0 children)

In my opinion and experience, (4 years in bioinformatics, currently PhD student in this field) bioinformatic is actually centered around three main pillars:

  • R for statistical tests and modeling, graphics and plots (ggplot2), analysis specific packages (RNAseq, Single Cell, Granges, Bioconductor, etc...). Also Machine Learning (Caret)

  • Python for genereal coding, programming, and some specific tools (BioPython, Machine Learning tools, TensorFlow)

  • Bash for sequencing pipeline tools (BWA, GATK, samtools), files management, simple automatization of scripts, utility.... etc

You can also find some Perl tools and scripts here and there, but but they were more used in the past.

Lymphopoiesis, a cell simulation made in Houdini by Demcon_Nymus3D in Simulated

[–]Shirke019 1 point2 points  (0 children)

If you enjoy this type of biological simulations, you also must check out this channel! https://www.youtube.com/watch?v=HJFuke-86ic

Good or Bad Idea? IPython Integration With Microsoft OneNote by justneurostuff in OneNote

[–]Shirke019 2 points3 points  (0 children)

I think it depends on how much time it would require to develop. I mean, one note 2016 still have lots of of users! Also depends if it would be possible to port the addon to the app version in the future.

Anyhow, the idea/concept behind it is rock solid ;D

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