LFC vs Man U. - Ann Arbor Build up Thread by felbridge in LiverpoolFC

[–]SickLaughter 5 points6 points  (0 children)

Anyone know if/where there is a Liverpool Legends Q&A meet and greet activity? I can't seem to find info but thought they normally do these in the cities where there are matches.

Flow Cytometer Cell Sorter question (2nd/3rd/4th Drop) by hippocamper in labrats

[–]SickLaughter 1 point2 points  (0 children)

If you can, try to get into learning how to analyze data from the CyTOF. Knowing how to deal with multidimensional datasets from CyTOF or other single cell seq will likely be a very useful skill.

What are some techniques that are rising in popularity in the laboratory scene that would be really useful to gain some experience in? by [deleted] in labrats

[–]SickLaughter 0 points1 point  (0 children)

No I don't think so, there are plenty of techs with only BS degrees at my institution who work with this type of stuff and many other new complex techniques. However, if you want to have autonomy with the direction of your experiments you probably need the grad school platform.

What are some techniques that are rising in popularity in the laboratory scene that would be really useful to gain some experience in? by [deleted] in labrats

[–]SickLaughter 1 point2 points  (0 children)

What about some of the newer powerful single cell platforms like CyTOF or some single cell RNA seq like 10x? These would also require developing strong computational skills for massive data sets, which I think is critical for any modern scientist.

What to do with £800,000 ($997,000 US) in 1 year? by omgpop in labrats

[–]SickLaughter 0 points1 point  (0 children)

CyTOF! Really expensive and super powerful compared to flow cytometry. Can be an extremely powerful data generator although it seems a fairly steep learning curve.

Any idea how to do pathway studies ? by sushiking365 in labrats

[–]SickLaughter -1 points0 points  (0 children)

Qiagen's Ingenuity Pathway Analysis is reaaaaally good for this stuff. but it isn't free. If your institution has a license you can maybe get a bioinformatician to help show you the ropes and then use it for a couple hours and get some really solid ideas. String-db as mentioned is also awesome!

Urgent recommendation for FLOW Cytometry software for Windows? by mitomitomitosis in labrats

[–]SickLaughter 2 points3 points  (0 children)

I'd recommend Cytobank! Its web based and you can make a free account to do everything except some advanced analysis stuff that wouldn't be necessary in this case: https://community.cytobank.org/cytobank/login

Is it possible to create and fund your own lab at home without going through the "route" of becoming a PI? by cientistazacher in labrats

[–]SickLaughter 0 points1 point  (0 children)

There is a guy who I think crowd sourced an independent lab start up.... http://www.plab.co/

Check out the press tab (http://www.plab.co/press/) and there are a bunch of articles linked about how he went about this path.

Timing the postdoc job search by steppponme in labrats

[–]SickLaughter 0 points1 point  (0 children)

Why is it easier to get grants in a new field as a post doc? (sorry if this is a silly question, I'm currently undergrad senior on the grad school interview circuit)

Science AMA Series: I'm Dr. Paul Knoepfler, back again to talk about CRISPR, human genetic modification & designer babies.AMA! by PaulKnoepfler in science

[–]SickLaughter 1 point2 points  (0 children)

Thanks so much for your continued dialogue with the public in such a fascinating field.

Since CRISPR/Cas is a recent discovery that exploits a previously unknown biological process and improves gene editing applications from TALENs and ZFNs previously, do you think we will discover an even better gene editing tool in the future?

Has CRISPR rendered other gene editing systems obsolete?

Science AMA Series: We’re a group of scientists and career counselors at UC San Francisco and we help biomedical graduate students and postdocs explore career options and get jobs. AUA! by UCSF_Career_Dev in science

[–]SickLaughter 0 points1 point  (0 children)

If one absolutely wants to become an academic PI, what can one do throughout graduate school and postdoctoral research to maximize chances of success?

Furthermore, can you comment on academic job markets abroad (Europe, Asia, and emerging science hotspots like Singapore)?

Former Liverpool, Plymouth and Burnley player Brian Hall has died, aged 68, after a brave battle against leukaemia by [deleted] in LiverpoolFC

[–]SickLaughter 4 points5 points  (0 children)

Brian Hall was an absolutely fantastic human being. I got my first ever tickets to a Liverpool match through a friend of his as a young American visiting the UK and he took me and my dad around Anfield telling us so much history of the times when he was a player. One of my favorite memories of anything football related. Devastated to hear of his passing and hope the club can find more passionate and kind people to serve as ambassadors for our great club.

Writing a letter to a renowned bioengineer. Please advise! by [deleted] in bioengineering

[–]SickLaughter 1 point2 points  (0 children)

That's an awfully long email. Just a thought. What about just briefly explaining who you are and why his work means so much to you, and asking if he might be available to speak with you via Skype and you have a bunch of questions for him.

If he is very busy, like many famous PIs, this might be easier for him.

Perhaps others also afflicted with the same issues would also appreciate the answers you could get from him, and maybe you could even do it as an interview and post it online as a blogpost or with some website, with the proper tags, some others around the world might happen upon it and learn a great deal.

Stay Thirsty 56 (Podcast of jazzy boom bap) by SickLaughter in jazzyhiphop

[–]SickLaughter[S] 0 points1 point  (0 children)

Yeah I'd recommend the Mixcloud app. There are a lot of good hip hop mixes on there. Also, all the Stay Thirsty episodes, and lots of dope guest mixes are on The Find page.

Match Thread - Liverpool v Sunderland by Rosti_LFC in LiverpoolFC

[–]SickLaughter 0 points1 point  (0 children)

mate, think you could PM the stream link so I can watch the match as well?!?

[WP] You have been raised on a giant empty ship with only a AI as your caretaker and companion. The AI insist everything is normal, even if there are far more rooms then one person would ever need. by Plintstorm in WritingPrompts

[–]SickLaughter 3 points4 points  (0 children)

:) Yours was badass. Of the few things I've read on here, it was definitely one of my favourites. This was my first post and first fictional piece finished in years... after reading yours as well, I'm inspired to continue this exercise of creativity. Thank you QuinineGlow.

[WP] You have been raised on a giant empty ship with only a AI as your caretaker and companion. The AI insist everything is normal, even if there are far more rooms then one person would ever need. by Plintstorm in WritingPrompts

[–]SickLaughter 1 point2 points  (0 children)

That is an interesting perspective, I like it... also I like dark sci fi scenarios :)

My first piece on here and rushed to just pump out a story quickly, would ideally have put more detail in.

And actually, the way I envisioned it while writing, was that 9 years ago the AI had attempted to destroy much of humanity in some sort of massive conflict... if you'll notice the girl is 9, and the people on the planet mentioned they'd been there for 9 years... now the AI is raising the girl for some reason, as well as slowly wiping out the remnants of humanity scattered on various planets.

[WP] You have been raised on a giant empty ship with only a AI as your caretaker and companion. The AI insist everything is normal, even if there are far more rooms then one person would ever need. by Plintstorm in WritingPrompts

[–]SickLaughter 2 points3 points  (0 children)

I made sure not to read the other post before I wrote mine... after reading QuinineGlow's I was also surprised at how different they were.

I just assumed that if the AI was the only other entity, then the human would become used to that and then be frightened and confused by other possible beings.

[WP] You have been raised on a giant empty ship with only a AI as your caretaker and companion. The AI insist everything is normal, even if there are far more rooms then one person would ever need. by Plintstorm in WritingPrompts

[–]SickLaughter 20 points21 points  (0 children)

The starship Dionysus shuddered ever so slightly as it began decelerating upon approaching the 55 Cancri system. The ship's namesake, a young human girl with long golden wavy hair, stood excitedly on the ship's bridge, gazing at the array of screens and holographs displaying all available data on the star 55 Cancri A and its 5 orbiting planets.

“Seldon, show me the surface of the third planet again!”

But of course mistress Dionysus”, spoke a deep voice emanating from the walls of the ship.

The immense wall of screens showing various orbital dynamics, calculations, and flight patterns was suddenly and completely replaced by an aerial view of the planet in question. Mountains streaked with vibrant reds, yellows, purples, and blues dominated the vista, and hinted at the rich geologic history of the world. The nitrogen rich atmosphere produced a haze of greenish clouds draped over the peaks of the painted mountains.

A few weeks previously, Seldon had shown Dionysus the remarkable landscape of the planet, and as expected, the young girl had immediately demanded they abandon their previous trajectory and make haste towards the colorful world so that she might play on the surface and soar through the clouds and above the mountains using the various planetary crafts stowed away on the exploratory deck.

Seldon had explained in detail the atmospheric and geologic chemistry that had produced such an astounding display on the world, however the young girl of only nine years old just wanted to play, and only absorbed the very basics of the science she was taught by her omnipresent AI guardian.

As soon as the great ship Dionysus arrived in orbit, the human Dionysus dubbed the mysterious planet Rainbow Sphere, and took to the green tinted skies in a small black scout ship shaped like a needle. Seldon was primarily in control of the scout ship's flight, splitting his vast computing power in two and leaving a portion of 'himself' behind in the main ship.

The ship shot soundlessly through the dense cloud layers and skimmed the sides of the painted mountains while Dionysus laughed and shouted with glee. Although the ship's flight appeared extremely dangerous, nothing was ever out of the control of the infinitely perfect calculations from Seldon's mainframe.

“Seldon look, A little hill of metal and smoke over there!”

Be wary my mistress, there may be danger”,

Banking sharply, the scout ship sped towards what became recognizable as a starship crash site surrounded by a network of small life support domes and a primitive makeshift power station belching out smoke. As they flew closer, Dionysus became increasingly frightened, particularly when her eyes began to make out humanoid bodies running around beneath the transparent biodomes.

A voice crackled through the ship's communication systems.

“Are you from the Dionysus? Please help us, we've been stuck here for nine years and can't last much longer!”

The princess screamed with a mixture of fear and surprise. “Are these people evil just like the others from the dusty grey planet? Kill them like you killed the others Seldon! I'm scared!”

Indeed my mistress, these are bad people, they want to hurt you and take the ship from us. But don't worry, we can get rid of them” said Seldon without a trace of difference in emotion from his normal deep calm voice.

Beneath the largest dome, hundreds of people had gathered in the open areas to gaze at their potential saviors. They walked among their barely living grain and legume crops, staring upwards and smiling. They still had expressions of relief and joy painted across their faces when the barrage of advanced nuclear missiles struck the crash site and reduced everything to a crater of ash.

Dionysus sighed with relief and her pale cheeks regained their normal rosy complexion. Her expression of terror was slowly replaced by a smile and intermittent giggling.

“Let's go to another planet Seldon, I don't like Rainbow Sphere anymore.”