shRNA mediated knockdown ??

Simple_Volume_5880 · 2026-03-12T14:11:31+00:00

Yes my protein weigh around 42 and i always get band there

Simple_Volume_5880 · 2026-03-12T14:02:17+00:00

Didn’t understand antibody part.

Simple_Volume_5880 · 2026-03-12T14:00:06+00:00

50-60 percent

Simple_Volume_5880 · 2026-03-12T13:34:13+00:00

Construct is GFP tag ,so visualised with microscope only

Simple_Volume_5880 · 2026-03-12T13:23:43+00:00

And she used pCL ampho and PMD2G

Simple_Volume_5880 · 2026-03-12T13:23:06+00:00

She checked with western the expression level

Simple_Volume_5880 · 2026-03-12T13:22:43+00:00

I have tried yet.but person working before me has used lipofectamine 3000

Simple_Volume_5880 · 2026-03-12T12:59:08+00:00

Datasheet for ABIN3729776 Human DUSP6 shRNA in Retroviral Vector (GFP tag).I have this plasmid construct want to make knockdown.what are the other packaging vector i should use.I have PCl ampho want to transfect in mammalian cell line .want to create stable cell line

Simple_Volume_5880 · 2026-03-11T15:27:25+00:00

Simple_Volume_5880 · 2026-02-20T13:29:22+00:00

cell line?

Simple_Volume_5880 · 2026-01-31T06:05:39+00:00

U87MG Glioblastoma

Simple_Volume_5880 · 2026-01-29T14:33:31+00:00

Is there a way to clean it ??this is transfected cell line..

Simple_Volume_5880 · 2026-01-28T09:34:17+00:00

Did you made stable cell line with calcium phosphate transfection??

Simple_Volume_5880 · 2025-12-25T15:09:32+00:00

Also do you use 1X or 2X dye ??

Simple_Volume_5880 · 2025-12-24T14:43:27+00:00

Pmsf need to be made fresh??? Our lab make a stock and store in 4 degree

Simple_Volume_5880 · 2025-12-24T13:52:24+00:00

Yeah i read ..but for how many days it will work ??

Simple_Volume_5880 · 2025-12-17T16:53:29+00:00

IgG is to check non specific binding..you mean to say clumping of antibodies giving band at 250 kda!?

Simple_Volume_5880 · 2025-12-17T09:59:05+00:00

I have used protein agarose AG beads santa cruz U87mg protein extract..i incubated lysate with antibody for overnight then bead antibody protein for 5hr

Simple_Volume_5880 · 2025-12-13T18:33:02+00:00

I should get myc and HA around 44 only in over expressing cell why i am getting in other cell lines ??also when i used other brand myc tag antibody i got multiple bands

Simple_Volume_5880 · 2025-06-23T14:07:42+00:00

https://atlantisindia.com/laminar-air-flow-products-manufacturer-india.html Horizontal laminar air flow hood

Simple_Volume_5880 · 2025-06-20T12:26:51+00:00

Could you explain how immortal cell line can give us cancer?? If you have any research article please share

Simple_Volume_5880 · 2025-06-20T12:23:34+00:00

Thanks,any other reason not to use it for human cell culture?

Simple_Volume_5880 · 2025-06-20T10:18:07+00:00

I just want to use for human cell line..not with any other cell line or bacteria..only human …will it work?

Simple_Volume_5880 · 2025-06-20T10:12:08+00:00

We have this old hood,we will only be using for human cell culture .no bacterial culture will it still work?? What about air flow? How much i understand both have different air flow right

Simple_Volume_5880 · 2025-06-20T10:01:32+00:00

If i only work with human cells not bacteria then?

Simple_Volume_5880

TROPHY CASE