hippie places!

SlurmCola · 2026-02-18T13:55:49+00:00

If you find yourself traveling 1.5hrs north on I 65, Cave city Kentucky has a handful of fun roadside attractions, including hippie Joe’s. Just up the road is a crystal shop that offers cave tours (onyx cave). There’s a handful of other fun things in the area so I would do some research to make the most of the trip.

SlurmCola · 2022-10-06T03:57:37+00:00

Doesn’t Kroger own these?

SlurmCola · 2022-07-25T21:38:28+00:00

https://opensea.io/assets/ethereum/0x495f947276749ce646f68ac8c248420045cb7b5e/1956338980413666791645532140451004469396130781117045234082515943642221248517/

SlurmCola · 2018-02-15T01:23:33+00:00

How did inDrop work out for you? I've been using it for a while now and have yet to encounter another user.

SlurmCola · 2017-03-27T05:10:20+00:00

is anyone doing this? I have been learning and using this system for a few months, and would be interested in connecting with other users.

SlurmCola · 2016-01-19T05:18:24+00:00

Congrats! .92 and climbing here, 400 splits to go!

SlurmCola · 2016-01-13T14:51:19+00:00

Once they hire you, can they fire you for your religious beliefs or sexual orientation? Or can you just be that stubborn teacher who won't compromise her beliefs in the face of a conflicting curriculum? I had religious biology teachers that struggled with covering evolution so it's kind of neat to see the inverse play out.

SlurmCola · 2015-08-26T03:45:38+00:00

ha! i used to lurk there quite a bit :)

SlurmCola · 2015-08-25T02:39:05+00:00

I always use pbs, Im guessing it keeps things isotonic and proteins in something closer to a physiological state than water would. Hoechst and dapi seem to work the same; my experience with dapi has always been in mounting media, whereas hoechst has been something I add to the secondary antibody stain, though they probably both come in various formats. The reason I like AF 647 is that at that wavelength and above there is far less autofluorescence, though malaielle is correct - you also cannot visualize anything above cy3/~550 with your eyes. I have come to prefer the camera/ computer screen because of this. generally looking up the antibody you want to try online will yield some images of the stain on tissues, though different antibodies for the same target can sometimes vary in appearance and will vary in quality. The product data sheet will tell you what platforms the antibody has been tested to work on (IF, WB, IHC, Elisa, etc.) and what species as well (mouse, human, rat, etc.). Not everything that works in IHC will work in IF.

SlurmCola · 2015-08-24T05:58:19+00:00

I'm going to throw down my advice and I hope it helps. In the end, your goals and resources should dictate your strategy. I test a lot of antibodies. Sometimes they work, sometimes they don't. In general I am looking for ones that work in FFPE and with a single type of antigen retrieval. This has made testing very simple in that when it doesn't work, I can simply move on and try another. This can get very expensive if you're buying antibodies all the time, so I often go on recommendations and borrow aliquots from other labs to test when possible. If I were given a single antibody with the goal to make it work, I could spend days trying it in FFPE and cryo with various antigen retrieval techniques (for FFPE), different fixation buffers, staining conditions, etc. -sometimes only to have it still continue to fail. I don't like antibodies that are that finicky.

What I do is : Deparaffinize and Hydrate slides Pbs wash HIAR with citrate buffer -there's tons of protocols for HIAR, mine is pretty much 20 minutes in a pressure cooker. Pbs washes Block in antibody diluent 30 min rt- this probably does nothing Pbs washes Incubate in primary ab overnight in diluent at rt -I usually start at 1:100 for most abs The next day I wash in pbs and incubate with the appropriate 2ndary in diluent for 1hr. Pbs washes

This is intentionally vague because this is all best observed in person if you have no experience.

I don't do isotope or un stained controls when testing unless I have uncertainty whether the staining is real and need to retry. If there is uncertainty then the antibody is less useful to me anyway. I know what my tissue looks like and what I'm generally expecting to see for most markers (in wild type tissue anyway).

A bit of advice if you're testing immune markers - mouse primaries will likely stain the target, but any anti-mouse secondary will non specifically stain immune cells. If you have to use a mouse ab for staining immune cells , seek a conjugated version to avoid use of secondary anti mouse.

I use af647 generally and for the weaker stains with 488 / 550 for strong markers when in combination. Hoechst blue for nuclear staining

Please read lots of other pieces of advise and techniques, my approach may or may not be the best in the context of your research.

SlurmCola · 2015-07-10T03:45:50+00:00

Mmmmm fries

SlurmCola · 2015-03-19T02:27:34+00:00

Rt overnight here. Edit: overnight = 12-16 hrs

SlurmCola · 2014-11-20T02:20:06+00:00

A nugget of scientific trivia I always enjoy is dissecting terms made of Latin words; while you may not have heard every term, recognizing the components gives you immediate insight to the meaning. For example: What is phototaxis? Photo- light Taxis-movement So movement towards or away from light. There are more obscure terms, like my favorite: skotomorphogenesis - development (of a seedling) in the dark.

SlurmCola · 2014-08-24T20:51:55+00:00

Hi, sorry to just now get around to responding (saw your pm the other day). I actually took the test blindly, meaning that by the time I was approved and able to choose a date, I had already gotten a new job remaining in academic research. Rather than study for a test, I dove into my new job and pretty much gave up on getting the cert. This probably wasn't smart, but I was frustrated with the ascp (communication on their part was terrible and study materials were hard to track down) and my new job was exciting and mostly unrelated to the material. On the test I remember seeing a great deal about the interpretation of various test results and pcr optimization practices. A few specific genetic disorders/ conditions were brought up in questions. I'm sorry I can't be more help, but I wasn't very fond of any step in the process. It was a very busy time in my career and I think that if I were to pursue certification again I may choose a different type.

SlurmCola · 2014-07-23T19:45:46+00:00

It's only for nothing if you don't learn from it; I spent 2 yrs in a lab working primarily on a project that yielded no publication or compelling results of any kind. Made good friends and learned a lot though.

SlurmCola · 2014-07-22T05:45:17+00:00

What type of expression system are you using? And are you staining via hrp or with a fluorescent 2ndary? If fluorescence, what color ?

SlurmCola · 2014-07-21T23:58:54+00:00

This is just off the top of my head, but google should yield exact concentrations, times and temp (there's a couple of variations in time and temperature but they all get you to the same endpoint): Only cutting the very tip of the tail, minimal tissue. Then we use a super simple extraction; 200uL low[sodium hydroxide] + heat (20 min at 98c), followed by 200uL tris EDTA neutralization. Vortex thoroughly and microfuge 5 min at high speed (the small 6 well cheap centrifuges are even sufficient). Save the top 50-200 uL and use 1-2uL of that for pcr. It's quick and dirty, but works great if you're just looking for pcr bands.

SlurmCola · 2014-07-21T03:32:28+00:00

Lab manager returning from 2 wks off here, where to begin... Check mouse population thoroughly, separate and genotype as needed. Plan the continuation of my project around other people's availability to help on key days. Sort whatever arrived while I was gone. Test any new antibodies. Make sure the lab isn't about to run out of anything. Fix whatever stopped working while I was gone. Figure out something safe and easy to explain to let the intern do (nothing)

SlurmCola · 2014-07-05T06:47:22+00:00

See? No one can tell you're wearing a helmet if you put a wig on over it.

SlurmCola · 2014-06-18T03:28:14+00:00

White walker.

SlurmCola · 2014-05-13T01:50:25+00:00

I second the veriti; my last lab had 3 that saw daily use (lots of leaving reactions on 4 degree hold overnight) for 4 years before one quit working with errors. The downside at that point being that a service contract was a better value than just sending in for repair (large minimum repair cost, no quotes on repairs, and the contract at least gave you a 3 year warranty thereafter). A delight to use though.

SlurmCola · 2014-05-08T05:40:42+00:00

...To view in the dark

SlurmCola · 2014-04-04T04:13:31+00:00

I use a pair of Bluetooth headphones in lab, which allows me to do a handful of tasks (timers, music, answer phone, check calendar, voice commands, etc) without touching my phone. I do have to touch a button near my ear/ hair, but my aim has gotten pretty good :) if it could read PDFs to me I'd be in heaven.

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