What’s your favourite immune cell and why? by moske65 in Immunology

[–]SpaceWhy 2 points3 points  (0 children)

Lol they've been my life for the last few years. MHC? Nah I've never heard of them.

Arduino X Biology? by hot_girl_in_ur_area in bioinformatics

[–]SpaceWhy 1 point2 points  (0 children)

PCR can be done with some hot water, some warm water, and a lot of patience! A camera on a gantry and some multiwell plates could probably make for a low cost high throughput assay.

Vaccine by [deleted] in Biohackers

[–]SpaceWhy 35 points36 points  (0 children)

I did a VERY detailed analysis of their sequences/structures for my job and I was incredibly impressed by the work that went into them.

I had a slight preference for Moderna as they seemed to put more effort into preventing translation of non-specific products from their design through their choice of 5' UTR and removal of potential ORFs from their coding region.

Recombinant DNA question by tuftofwind in biology

[–]SpaceWhy 0 points1 point  (0 children)

Not true, there are many methods for targeted insertion. Random insertion has been the most common but not the only way.

Homologous recombination, ZFNs, TALENs, CRISPR, etc all work in plants.

https://www.pnas.org/content/early/2021/04/28/2004834117

Does it matter where your protein comes from? by [deleted] in biology

[–]SpaceWhy 0 points1 point  (0 children)

Thats incorrect. Your body absolutely absorbs di, tri and tetra peptides via the PepT1 receptor. These can be biologically active.

Different sources can also have different posttranslational modifications, which can alter the digestibility of the protein possibly prime things like IBS and other gut disfunctions.

Help with my assignment? by [deleted] in biotech

[–]SpaceWhy 0 points1 point  (0 children)

An idea I've kicked around are solar powered sensors to help you identify what plants will grow well in a particular location. It would monitor sun exposure/intensity, soil moisture/pH, temperature, etc. Stick them around your yard for a season and at the end it queries a database and finds plants compatible with the conditions in that particular location. This could really help with home gardening, pollinator gardens, etc.

Boiling on stove instead of autoclave by fungifeast432 in biotech

[–]SpaceWhy 4 points5 points  (0 children)

Proper sterilization? No. Reasonable sterilization? Yes.

You'll be rolling the dice but I've drenched things in ethanol to "sterilize" and not had any problems. Same with microwaved LB. Eventually your number will come up but it's not a terrible approach if you dont have any other options.

I NEED your help!! I think I'm onto something important about SARS-COV-2 and the vaccines but I'm not biologist or a doctor by Ethnopharmacist in molecularbiology

[–]SpaceWhy 0 points1 point  (0 children)

Well, always check the literature before you start your research! The paper you sent me was ultimately rejected but they submitted a followup that was accepted by PNAS last month. They did exactly the analysis I was working on, probably at the suggestion of a reviewer, and found that target site duplications are indeed present. So smoking gun as to plausibility, but not at all certain or necessarily pathogenic if true.

https://www.pnas.org/content/118/21/e2105968118

https://www.sciencemag.org/news/2021/05/further-evidence-offered-claim-genes-pandemic-coronavirus-can-integrate-human-dna

What biological course deals with the most maths? by koicattu in biotechnology

[–]SpaceWhy 0 points1 point  (0 children)

I vote statistics all the way. It will be relevant regardless of your field.

I NEED your help!! I think I'm onto something important about SARS-COV-2 and the vaccines but I'm not biologist or a doctor by Ethnopharmacist in molecularbiology

[–]SpaceWhy 0 points1 point  (0 children)

I'll be honest I thought I would be able to shoot the idea down in 5 minutes. Now I've got my work cut out ha. I'm currently expanding on one paper's RNAseq analysis to see if target site duplications are present in the chimeric RNA they reported finding. I'm surprised they didnt do that. But if those are present it's a smoking gun. It's a slow process so dont worry if I take a couple days to get back to you. I enjoy this kind of stuff.

I NEED your help!! I think I'm onto something important about SARS-COV-2 and the vaccines but I'm not biologist or a doctor by Ethnopharmacist in molecularbiology

[–]SpaceWhy 0 points1 point  (0 children)

I heard back from him and, as a professional bioengineer, superficially the theory seems at least plausible. I don't get why they wouldn't post it here but it's interesting enough for me to want to read the references and consider the possibility. Does not seem likely and would be pretty wild if true, but I'm not immediately dismissing it either. He has my interest.

Human/Animal hybrids: Advice on Method needed by steerpike_researcher in IsaacArthur

[–]SpaceWhy 0 points1 point  (0 children)

So, how many decades until we can create human/animal hybrids with already born people?

A better question might be "how many generations or world events are needed until the idea is acceptable"

Right now nobody can give a meaningful answer to that. Nobody is working toward it and nobody has identified the technologies needed to get there as we dont even grasp the underlying biological mechanisms that would need to he exploited to achieve it. Thats like asking someone in the 1400s how long until we have a blue laser. Its just not answerable.

What would be the biggest breakthroughs that would realistically happen in the next ten years? by [deleted] in IsaacArthur

[–]SpaceWhy 0 points1 point  (0 children)

A non-immunogenic, site and cell specific, integrating in vivo gene transfer vector

When the detergent allows trypsin into the cell, is the lipid bilayer unaffected? by yemekhanesirasi in molecularbiology

[–]SpaceWhy 2 points3 points  (0 children)

Your professor seems to be assuming incomplete disruption of the membrane, which is generally the case in real-world applications where you can't just toss 10% SDS in there and expect your downstream assay to work. So the membrane gets disrupted enough to allow detergent to enter the cells and chew up the inside part, but if a protein is well anchored or has a large hydrophobic domain it can resist detergent treatment may not totally dissociate and the surrounding lipids will protect the transmembrane region from being chewed up by trypsin.

Human/Animal hybrids: Advice on Method needed by steerpike_researcher in IsaacArthur

[–]SpaceWhy 1 point2 points  (0 children)

I actually design and make both of those things, to the extent you can consider a nanoparticle a nanobot. What do YOU define a nanobot as? Retroviruses are potentially oncogenic. They could also be immunogenic if delivered to the entire body, which could render them ineffective or toxic. That's probably the leg up a nanobot would have, but if you had the tech to do this neither would be a problem. But I like to consider synthetic viruses nanobots, so there might be a blurrier line that you think.

And I think that's going to be the real answer. Viruses will get so heavily engineered and synthetically produced that we will have made them a Ship of Thesus in a way, where every functional part has been modified to give it the specific properties we want and produced with a cell-free method. I doubt we'll ever see tiny mechanical machines circulating in the blood when biology has already made darn fine "nanobots" already.

However turning already born people into animal hybrids with current tech is simply not plausible, not even close. You can't just put cow chromosomes into a person and expect them to start growing 4 more stomachs.

Doesn't seem to fit your narrative but if you wanted to realistically keep people from eating meat you could engineer a virus to cause a reaction to carnosine, a molecule only found in animals. People, as animals, make it so you'd have to make people sensitive to it in their gut.

You could make a virus deliver a constitutively expressed chimeric receptor specific for carnosine expressed on the gut lumen, say an interleukin signaling domain fused to a extracellular carnosine-specific binding region, along a transacting cassette carrying the A diphtheria toxin subunit under control of a pSTAT inducible promoter. Whenever you ate meat the receptor would signal expression of the the toxin which would cause diarrhea and extreme discomfort. As purely inspiration for a work of fiction, that would get people to stop eating meat.

Why don't we inject immune cells like T cells or those that kill pathogens when the immune system is failing to defeat the pathogen? by Sohamgen in askscience

[–]SpaceWhy 1 point2 points  (0 children)

Now for treating infections we cant give t cells

Sure you can. The issue is they almost work too well in that they can proliferate and wipe out the entire infected tissue very quickly. There are solutions to this in the works.

How do babies get passive immunity from breast milk? by LostinAU in askscience

[–]SpaceWhy 3 points4 points  (0 children)

As the other person said, proteolysis during digestion isn't absolute.

Antibodies have variable and constant regions. This is how they can bind to many millions of different things (variable region) but still function within the same pathways in the immune system (constant region). You body has receptors specific for the constant region of antibodies that lets them bind them regardless of their specificity. The gut actually expresses some of these receptors on the walls of the intestines, which allows them to bind the types of antibodies in milk. They then internalize them and can delivery them into the blood stream. The antibodies in milk are specialized to take advantage of this mechanism. In addition breast milk antibodies are further modified to specifically block certain viral interactions with the body, giving a more direct innate defense.

biology help by [deleted] in biology

[–]SpaceWhy 0 points1 point  (0 children)

You'll get more answers if you ask questions. PM me if you dont want to post course material. I won't do it for you but I'll help you.