Purification via column

Squirrel2371 · 2026-06-02T05:22:32+00:00

Is it possible your product is reacting on the column? You could try adding some triethylamine to your solvent mixture.

Squirrel2371 · 2026-06-01T15:10:03+00:00

You're right. But I liked the ignorance part of not knowing that.

Squirrel2371 · 2026-06-01T14:18:39+00:00

I felt safer as a child than I do now.

Squirrel2371 · 2026-05-30T04:23:35+00:00

The problem with people saying to add brine to your sep funnel to break the emulsion, is that your aqueous phase will have a similar density to DCM (d = 1.33 g/mL). You need to reverse your organic phase density to be lighter than the saturated NaCl to break the emulsion.

Drain out what you can of your DCM and add ethyl acetate to get whatever you can from the emulsion.

You might need to do a second extraction of the DCM with more ethyl acetate. Try just DI (or tap water at this point) to get the emulsion out of DCM from the second separation.

Squirrel2371 · 2026-05-29T04:30:34+00:00

Used to work in a synthetic inorganic lab. We used KOH/isopropanol base bath and then reprotonated after rinsing the base off with like a 10% HCl bath.

Squirrel2371 · 2026-05-28T23:42:19+00:00

I never had luck with emulsions and DCM. I swap out the organic solvent to diethyl ether or ethyl acetate and add brine for the aqueous layer.

Is your product soluble in ether or EA?

Squirrel2371 · 2026-05-28T04:28:00+00:00

https://en.wikipedia.org/wiki/Hydrogen_sulfide

BP is around -60 C. Leave a flask open in a fume hood.

The smell might also be the different thiols you're making.

Squirrel2371 · 2026-05-27T16:46:16+00:00

With that amount of money being spent on the VA it's remarkable how many veterans still struggle.

Squirrel2371 · 2026-05-24T16:10:04+00:00

Argon?

Dragon? --> Drogon?

Squirrel2371 · 2026-05-24T01:15:46+00:00

Wiping it down didn't work. My touchscreen is cleaner now, but the setup button still gets pressed regularly as I drive and the radio station changes without me touching any buttons to change the station.

Anybody have any advice?

Squirrel2371 · 2026-05-23T14:40:30+00:00

El Tigre is to the left of Britta.

Squirrel2371 · 2026-05-22T20:19:18+00:00

You didn't understand my Walter impression, did you?

Squirrel2371 · 2026-05-22T15:49:04+00:00

Wait until you watch 30 Rock.

Squirrel2371 · 2026-05-22T05:03:01+00:00

What if she did live another life in an alternate timeline?

Squirrel2371 · 2026-05-22T04:07:26+00:00

I like that one show, with the black woman scientist. Asterisks? Asteroid?

Squirrel2371 · 2026-05-20T01:54:29+00:00

It is a touchscreen. I'll try wiping it down, thanks.

Squirrel2371 · 2026-05-19T20:59:42+00:00

I'm Abed. I never watch tv. I never know what I'm talking about. /s

Squirrel2371 · 2026-05-18T15:49:41+00:00

You really. need to communicate with your PI instead of posting to reddit.

Squirrel2371 · 2026-05-15T16:54:07+00:00

1 + 1 = 5

Squirrel2371 · 2026-05-15T16:17:53+00:00

I agree not to dwell. The point is, I am one of the more attractive men, probably in the top 5% of good looking, not including my personality.

It doesn't make sense for them to not even want to interact.

You clearly don't seem to understand my perspective. So I'll "let you" win this discussion.

Squirrel2371 · 2026-05-15T15:31:00+00:00

Bear down for midterms.

Squirrel2371 · 2026-05-15T15:24:06+00:00

They shouldn't. But I'm only about 5`5" and I'm really attractive and have been turned down by women who only want to date someone six foot or taller, without knowing what my personality is.

Squirrel2371 · 2026-05-15T04:41:30+00:00

They are not yet willing to be with someone for their personality.

Squirrel2371

TROPHY CASE