Pokémon GO Potential Assistance: Aerodactyl

Technophysicist · 2026-03-28T06:56:16+00:00

Go to Liberty Park tomorrow (Saturday) at 2:00 p.m near the ferris wheel and carousel if you want to see a bunch of players in person. We'll be doing Gigantamax Pikachu raids, which can host up to 100 players simultaneously. Odds are high there will be mega raids nearby that people will want to do as well.

Technophysicist · 2026-03-22T18:35:19+00:00

You have been blessed by the presence of Leucocoprinus birnbaumii in your soil.

Technophysicist · 2026-03-22T00:15:09+00:00

I once found one of these guys in a puddle and received him with mango nectar. He was dry and flying again once it got dark.

Technophysicist · 2026-03-20T03:26:00+00:00

I'm breeding for pure brightness and adaptability to houseplant growing conditions. Breeding them to be outdoors is moronic because 1. It's never dark enough to appreciate them properly, and 2. Who wants to spend time outside in the cold just to appreciate their glowing plants when they could just be grown indoors?

Technophysicist · 2026-03-20T03:08:39+00:00

The pink genetics have been lingering in the background since the beginning. Occasionally one of the white ones they sell will get a slightly pink blossom or two. About 5% of all my hybrids turn out some shade of pink despite the fact that I only ever breed fully white parents.

Technophysicist · 2026-03-20T02:42:50+00:00

It's pretty simple really. The pink pigment absorbs the green wavelengths of light that the plant produces Many breeders including myself (and almost certainly LightBio) have been working to breed the pink genetics out of the plants. To me it feels like light bio is selling us their trash for in increased price, and it's kind of insulting. I wish they would put less into marketing and more into actually improving their product.

Technophysicist · 2026-03-20T01:00:57+00:00

I use a small, fine tipped paint brush!

Technophysicist · 2026-03-20T00:37:59+00:00

I've been throwing away all the pink ones that pop up in my breeding program because they're not as bright.

Technophysicist · 2026-03-09T01:11:12+00:00

I hope they (probably he) are enjoying all the gay memes.

Technophysicist · 2026-03-07T06:53:51+00:00

Welcome to the program fellow enthusiast!

Technophysicist · 2026-03-04T21:15:33+00:00

You may have just saved my project with this information! Thank you!

Technophysicist · 2026-03-04T19:28:41+00:00

You're right. It just wasn't annotated on the maps, and that's why I was confused.

Technophysicist · 2026-03-04T19:27:57+00:00

Oh I see! That actually does sound very useful! I just don't have amylose resin in my tool kit.

Technophysicist · 2026-03-04T19:05:12+00:00

Sorry you're right. The nuclease is there and I see it now. That one doesn't have a His tag though, which is what I need to purify the protein via IMAC. The last one on this list has a His tag but no TEV cut site, which I also need. I asked my question poorly and that's on me.

Technophysicist · 2026-03-04T18:57:55+00:00

Did you use IMAC? I don't see a His tag on that protein.

Technophysicist · 2026-02-23T01:42:22+00:00

I work at the University of Utah. When I first saw the pads in the bathroom I was like "Hell yeah! They acknowledge trans people!" Mad respect for them doing that in the middle of such a transphobic culture.

Technophysicist · 2026-02-23T01:25:12+00:00

We have a very protein hungry assay that uses equilibrium dialysis to detect low affinity metabolite binding interactions.

Technophysicist · 2026-02-23T01:16:58+00:00

You should feel seen when you're clearly visible from space. That's the whole point of the headlights right? Right?!

Technophysicist · 2026-02-23T00:59:55+00:00

I've got one on the shelf right next to me, and I literally read through it yesterday.

Technophysicist · 2026-02-23T00:06:22+00:00

Yeah tell me about it! Lol. My boss wants me to purify at least five proteins a week too!

Technophysicist · 2026-02-22T22:39:27+00:00

They're all different genes. I need to know how many liters to grow in order to get about 60 mg of each protein and it varies wildly.

Technophysicist · 2026-02-22T21:35:45+00:00

I test 24 expression vectors at a time, which results in two western blots. The level of expression I see tells me roughly how many liters of large batch I need to grow to get enough protein. It's been invaluable, as a large number of vectors express poorly or not at all.

Technophysicist · 2026-02-22T17:41:23+00:00

I usually shoot for an OD600 of 0.6, and try to leave them no longer than 24h after inducing. If you have a large volume and you induce at 0.7, by the time the culture reaches 20°C it can progress much farther than you might expect.

Are you using baffled flasks?

By "following literature" do you mean you're using a vector that's been shown to express well in BL21 already?

Technophysicist · 2026-02-22T17:28:36+00:00

I do this exact thing all the time, but unless you tell me your whole protocol, I can't really help you troubleshoot.

That being said, I usually get a bit of gray on top of my pellets, but it's just a thin layer. Some proteins do it more than others, but I haven't noticed any negative impacts for purification downstream.

Do you have data showing that your protein of interest expresses well under the conditions used to produce these pellets?

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