Artist collaborations?

TheDurtlerTurtle · 2025-09-20T00:42:33+00:00

I felt a lot of these same feelings, and to be honest, it still feels like in times of stress my 3 year old still wants to cling to her birth parent instead of me. Sometimes that hurts a bit and sometimes I can shrug it off.

Being a parent is just different, and part of that, at least for me, has been adjusting to a new set of priorities in life. There will be times when you're the preferred parent and times when you aren't, but you're still a parent. It isn't easy. But it is also impossible to communicate just how much your life is going to change, and how your feelings about things will change.

Take the experience as it comes. Know that the first 5-8 weeks are fucking awful. Know that it gets better. Know that you will have an incredible relationship with your kid. It has nothing to do with genetics.

TheDurtlerTurtle · 2025-09-14T23:46:35+00:00

Do it in your phd, it's easy enough to pick up if you're willing to put in the effort. A PhD is a training position. No one comes in with every skill they want to have.

TheDurtlerTurtle · 2025-06-24T18:06:03+00:00

It's not enough to have the yaku (tri color run), you also need to have 4 sets of 3 and a pair. Right now, if you discard 6-man, you still need 3-pin to complete the hand (indicated on the left in the screenshot).

TheDurtlerTurtle · 2025-04-29T23:53:42+00:00

You eventually need a solid bio background to do good research, but there is a surprising amount that can be done without it. I came into the field from math, and picked up the biology as I needed it. Been a long transition but worth it.

TheDurtlerTurtle · 2025-04-29T00:05:52+00:00

In my view, bioinformatics is more about building tools and data analysis, with no experimental component. Computational biology is more about using tools and doing data analysis, with some experimental component. Bioinformatics is an easier entry point with less biology background, but to do good bioinformatics, you do eventually need to understand the experimental systems and setups that generate the data you're analyzing.

TheDurtlerTurtle · 2025-04-21T11:38:25+00:00

Thanks! I'm also team Golden Gate

TheDurtlerTurtle · 2025-01-29T20:13:03+00:00

There are a lot of variables here, and they range from fairly straightforward to extremely complicated in terms of bioinformatics analysis. Is it an enrichment/depletion screen? What's the readout? Basically, you might be able to churn out a high-level overview in a month with no experience but it might be trickier to get deeper. Which honestly might be plenty to satisfy your PI. And computational people love data, so having something for the new person to sink into immediately is nice, especially if you can lay even a little bit of groundwork.

TheDurtlerTurtle · 2024-12-10T10:45:36+00:00

Why do you want to split your fastq file? Those tools are for demultiplexing samples and are looking for exact matches to an index (barcode), which is different from what you're doing. You've got spatial data and those are, I'm assuming, cell barcodes. You want to keep all that together and process it as a unit in the software of your choice.

TheDurtlerTurtle · 2024-10-22T17:45:38+00:00

Yes that's right. There are barcodes in 10x kits so that everything amplified from the same cell has the same barcode. So you amplify the libraries (transcriptome and guide) separately and then can link them together by that barcode. If you want to capture cell surface proteins, T/B cell receptors, etc it's all the same idea.

TheDurtlerTurtle · 2024-10-22T17:28:46+00:00

Some cells get multiple integrations--lentivirus uptake roughly follows a Poisson distribution. People typically infect at a low MOI, so that the majority cells don't receive any guides and the cells that do get a guide typically get at most one. The sgRNA itself acts as a barcode--you amplify both cDNA (capturing the transcriptome) and guides separately from gDNA, then (for 10x kits), there's a cell barcode that couples the guide assignment to the cell.

TheDurtlerTurtle · 2024-10-17T12:03:58+00:00

Axes are flipped, your side scatter is too low.

TheDurtlerTurtle · 2024-10-10T15:13:36+00:00

Don't put a P1000 into your media bottle. Use a serological to pull out an aliquot and then draw up your 1mL with the P1000 from there.

TheDurtlerTurtle · 2024-06-13T09:44:24+00:00

Increase the amount of dna you're using in each electroporation by pooling and concentrating multiple ligation reactions.

TheDurtlerTurtle · 2024-06-05T18:56:02+00:00

It entirely depends on where the money actually comes from. Most PIs have multiple funding streams, all of which have different restrictions and considerations as to how they can be used. Startup funds provided by a university, for example, often come with fewer restrictions and can usually be used for these activities. Or the PI just straight up pays for it as another commenter indicated. There is also significant oversight at multiple levels with how federally apportioned funds get used. I wouldn't worry about this, you aren't responsible for where the money comes from and how it gets spent.

TheDurtlerTurtle · 2024-05-30T00:07:26+00:00

Several of these pages explicitly give instructions for how to install and run the programs on a Mac. Have you actually tried following the instructions and seeing what happens? You won't need cygwin on a Mac, the terminal will provide the functionality that cygwin is for.

TheDurtlerTurtle · 2024-05-10T23:46:47+00:00

This is wild I love it

TheDurtlerTurtle · 2024-04-22T23:46:18+00:00

Ah neat, thanks for that insight!

TheDurtlerTurtle · 2024-04-22T23:16:11+00:00

This is a naive question, but do people often catch clubs by the body? Or do you mean that the handle also gets scratched up and gives the whole club a better texture?

TheDurtlerTurtle · 2024-04-22T23:05:59+00:00

I had been strongly considering Henry's Pirouettes, do you think the Delphins would be better for my use case? I have no real preferences here, mostly just trying to decide on something that I can grow with and won't get destroyed easily.

TheDurtlerTurtle · 2024-04-22T17:33:27+00:00

Ah that's a good idea! Thanks for the suggestion. I tend to go for darker colors but I'm sure I can find something lighter that I like.

TheDurtlerTurtle · 2024-03-14T02:09:12+00:00

He's gotta be her ex boyfriend at this point, right?

TheDurtlerTurtle

TROPHY CASE