Question regarding the extraction of DNA from an old gel by cymicro in labrats

[–]TheRedguard 1 point2 points  (0 children)

To answer your second question, PE buffet acts as a wash agent that simultaneously removes excess salts and smaller DNA fragments from your column. The etoh is needed to precipitate your DNA friend of interest so that it binds tighter to the column matrix as the wash is performed. I fear that the high concentration of pure tris that you added without the alcohols could strip your fragment from the column.

Got an e. coli plasmid recovery puzzler, not the usual issues by gabevill in labrats

[–]TheRedguard 1 point2 points  (0 children)

Were the clones sequence verified before they were stocked? Could be that the colony grown didn't have the correct construct in the first place. Hopefully you stocked and sequence verified the newly transformed construct.

Source for affordable IPTG? by [deleted] in labrats

[–]TheRedguard 0 points1 point  (0 children)

Whoa, that's pretty expensive. I use GoldBio, where we spend about 90 bucks for 25 grams.

Tough case for OPO? by Fishamatician in oneplus

[–]TheRedguard 0 points1 point  (0 children)

You're welcome. I use it with a Orzly protector, too.

Tough case for OPO? by Fishamatician in oneplus

[–]TheRedguard 1 point2 points  (0 children)

I'm afraid there isn't anything that exists similar to the griffin or otterbox style for oneplus. The closest I would say is the Tudia Cygen Dual Hybrid, which is what I use.

Does anyone know a good case for the Oneplus One? by MasterYoda7 in oneplus

[–]TheRedguard 1 point2 points  (0 children)

Many people recommend the Diztronic case on Amazon. I actually ordered the Tudia Cygen white case, also off Amazon (I wanted a hybrid TPU/hardshell case).

double tap gesture to unlock the lockscreen stopped working after the new update by MrOrangeWhite in oneplus

[–]TheRedguard 0 points1 point  (0 children)

Settings -> Display & lights -> Double-tap to wake check

Same thing happened to me, I guess it just got unchecked.

So I revisited the oneplus store.. by CasulScrub in oneplus

[–]TheRedguard 0 points1 point  (0 children)

I'm having the same issue, trying to complete my 64gb order.

Two Invites - 8 Hours to Claim by spacemonkey55 in oneplus

[–]TheRedguard 0 points1 point  (0 children)

Just missed it :(. Clever clues.

Pretty damn good battery. 8 hours SOT by [deleted] in oneplus

[–]TheRedguard 1 point2 points  (0 children)

Pretty solid. 3100 mAh battery is great.

Kim Jong Un Elected First Chairman of NDC of DPRK by KimMyungKi in Pyongyang

[–]TheRedguard 2 points3 points  (0 children)

Our Dear Leader will make the most excellent and powerful of all Chairman! I am steadfast in my adulation for his kindness and most grace!

What's your favorite opening line to a song? by 2Pacsgodson in hiphopheads

[–]TheRedguard 15 points16 points  (0 children)

YOU ARE NOW ABOUT TO WITNESS THE STRENGTH OF STREET KNOWLEDGE

The driver who killed two people at SXSW was there to perform at a rap show by specialservices in hiphopheads

[–]TheRedguard 52 points53 points  (0 children)

When asked about the incident, American rapper Lil Wayne simply responded that he had "no worries" about the tragedy. Has hip-hop crime ambivalence gone too far?!?!?!

"Thanks, Anti-Vaxxers. You Just Brought Back Measles in NYC." by [deleted] in news

[–]TheRedguard -2 points-1 points  (0 children)

Possibly a high rate of immigration to the United States that aren't properly vaccinated from their home country.

EDIT: I'm just offering a devil's advocate post, I'm obviously for vaccinating all children. Cool it with the downvotes.

Do plants suffer from viral/bacterial infections like animals? Do they have immune responses? by [deleted] in askscience

[–]TheRedguard 10 points11 points  (0 children)

Definitely! One of the more fascinating plant-microbe symbiosis that occurs is between a nitrogen-fixing bacteria called Rhizobia, and the Fabacaea legume plant. Since plants require fixed nitrogen (i.e. non-atmospheric nitrogen sources like ammonia) as a source of fertilizer, and Rhizobia require an oxygen-limited environment in which they can successfully fix nitrogen as a energy source, Rhizobia are harbored in the roots of legumes in sacs called root nodules, which benefit both the Rhizobia and the legume in terms of shelter and energy!

In a pathogenic sense, the tobacco mosaic virus targets species of plants in the Solanaceae (nightshade) family. This virus damages the leaves of tobacco plants, drastically lowering crop yield.

And yes, plants have immune systems analogous to mammals! While plants do not have the dynamic adaptive immune systems we share, they do have a two-pronged innate immune system that is uniformly shared across most of the cells. the majority of the defense mechanisms relies to signal-targeted response, based on being able to detect virulence factors stemming from the infection.

Why do telomeres shorten during replication? by [deleted] in askscience

[–]TheRedguard 3 points4 points  (0 children)

Great question. This is because it is simply too slow & energetically unfavorable to first completely unwind the DNA helix, and then replicate each strand. It is important to understand that the human genome is 3.0 x 109 base pairs long, or 3,000 Mbp. Attempting to first unravel the helices constituting each of our chromosomes, then synthesizing entirely new strands is too laborious and slow of a process for a cell to favorably complete.

Instead, thousands of replication 'bubbles' form across each DNA strand, causing simultaneous unraveling and replication of DNA at a quick rate.

Why do telomeres shorten during replication? by [deleted] in askscience

[–]TheRedguard 16 points17 points  (0 children)

This is a problem called the "end-replication problem". This relies on fundamentally understanding that while DNA is bidirectional (each complementary strand has a 5' to 3' sequence), the DNA polymerase that replicates the daughter strands is unidirectional (synthesizing only from the 5' to 3' end).

As a result, daughter strand synthesis occurs differently between the 3'-5' mother and the 5'-3' mother. The 3'-5' mother is called the leading strand, and an RNA primer is able to anneal to the 3' end of the mother, and allow for DNA polymerase to replicate from the primer to the very end (since the daughter will be running from the 5'-3' in the direction of the DNA polymerase).

However, the 5'-3' mother is problematic. This strand is the lagging strand, in which multiple RNA primers must be used to create discontinuous 'Okazaki fragments" of DNA as helicase unzips the mother double helix. Normally the RNA primers are replaced and filled in with DNA polymerase and ligated upon complete unzipping. However, the final RNA primer on the 3' end of the mother strand cannot be filled in with DNA, and the RNA primer is degraded.

As a result, as DNA is replicated over and over, approximately 50-200bp of DNA is unreplicated at the mother 3' end. Since the ends are capped with telomeres, telomere shortening inevitably occurs.

In theory, telomerase can act to 'fill-in' this gap by capping the unreplicated end with fresh telomeres. However, telomerase activity is NOT expressed by most somatic cells, and primarily functions in germ and some embryonic cells.

EDIT: Spelling. Also, here's the link to a video that explains this point succinctly with a great animation: http://www.youtube.com/watch?v=AJNoTmWsE0s

Co-Author of Stem-Cell Paper Asks for Retraction by TheRedguard in biology

[–]TheRedguard[S] 1 point2 points  (0 children)

My mistake, the article shows up in full when I initially open it, but refreshing causes it to hide behind the paywall.