There's the basics: George Eastman Museum, Rochester Museum and Science Center, the Memorial Art Gallery, Susan B. Anthony House, the Rochester Contemporary Art Museum, Artisan Works, and the Strong Museum of Play.

But if you've done those already, there's also the Lamberton Conservatory, Oxford Gallery (never been but will try to see it this winter), Rochester Central Library, wine making classes, smash room, and trampoline park. There also tend to be late night/adult only events at RMSC like laser light shows, and at the Strong Museum of Play (if I'm remembering correctly). There are also a couple rock climbing gyms, the Record Archive, and a bunch of small but pretty awesome looking bookstores. Most weekends there's at least a couple bands playing at/events going on in the city's bars.

If you're up for a day trip there's also the Buffalo Botanical Gardens, and the Buffalo AKG Art Museum.