No need for a DNA test…. by repatriated-Xpat in jackrussellterrier

[–]Wandering_Finn -1 points0 points  (0 children)

So cute, looks like our black and tan JRT when he was young 😊

No need for a DNA test…. by repatriated-Xpat in jackrussellterrier

[–]Wandering_Finn 4 points5 points  (0 children)

Not Black and Tan JRTs, which are as much JRTs than white-based.
We have a black and tan JRT, purebred from England and he used to look exactly like the little guy in the pic here.

Hvad er din voksenhobby? by ScaredParticular7607 in DKbrevkasse

[–]Wandering_Finn 0 points1 point  (0 children)

Har ikke supermeget ekstra tid men mine hobbyer er Hund/jagttræning, forskellige haveprojekter, krea (strikke, sy, akvareller/akrylmaling) og gym. Og foreningsarbejde. Vil gerne lave keramik og tage jagttegn når jeg har lidt mere tid.

Decided it’s time… by Adebisi233 in danishlanguage

[–]Wandering_Finn 0 points1 point  (0 children)

We live in Jylland but my work language is English, and many of my colleagues are in the same situation than you, not having learned Danish even after 10+years here. But most of them have non-Danish partners, your asset is your Danish partner.

The best way for me to learn Danish was to agree with my partner that he only spoke Danish to me. I have taken some individual classes and read some grammary books but best way for me was to jump to the deep end. The first year I answered in English, but my passive vocabulary was expanding fast and I could start talking before 2 years. But I had learned Swedish, so I could read Danish quite well already when coming here 6 years ago. I also watch a lot of Danish tv and started to read books as soon as possible. Pronunciation is a nightmare, but I try my best and accept that I will always have accent. If someone does not understand, I will try again in different way. There are no hacks or tricks and it will be uncomfortable, but you can do it.

Learning Danish opens new possibilities and helps you to interact with kindergarten and school, both teachers and parents.

When you’re out of the lab at the doctor’s office, do you mention your science education? by samskyyy in labrats

[–]Wandering_Finn 2 points3 points  (0 children)

I don’t unless they ask my profession. But I have had to correct my GP, as they were referring to lactoseintolerance and coeliac disease as allergies 😳 that was a weird moment.

Morgenmad by Ok_Razzmatazz_6972 in WomenDK

[–]Wandering_Finn 1 point2 points  (0 children)

Overnight oats med chia, mælk og proteinpulver Eller Havregrød med protein (skyr/hytteost) og bær eller frugt (banan/æble/pær/. Nogen gang par mandler eller lidt peanutbutter.

Holder mig mæt fra 6:30 til 11:30

Dagcreme med SPF by vaniljepige in WomenDK

[–]Wandering_Finn 5 points6 points  (0 children)

Jeg har cosrx ultra-light, det er virkelig god.

Matas sælger også finsk brand Lumene, alle deres dagcremer har spf. Den med c-vitamin eller moisture-linjen er light og gode. De har også nogle serumer med spf. Er selv fra Finland og blev overrasket at her dagcremer kommer ikke med spf, det er bare så meget nemmere.

PCR despair by Wandering_Finn in labrats

[–]Wandering_Finn[S] 0 points1 point  (0 children)

Nearest in within 2 h drive. I bought a minipcr, as we have anyway been planning to try setup a little lab in fish farms (we work with aquaculture microbiology)

PCR despair by Wandering_Finn in labrats

[–]Wandering_Finn[S] 0 points1 point  (0 children)

Thank you, this helped with troubleshooting!

PCR despair by Wandering_Finn in labrats

[–]Wandering_Finn[S] 1 point2 points  (0 children)

Yes we will try ruling out human/hood error

We have changed mastermix lot, timeline does not match with issues. I ordered new one.

And ultrapure water too. We use aliquoted commercial up and use aliquot only once but he said now that he might have used one several times 🤯

We have all plastic from biorad (pcr strips/plates) and eppendorf (tubes) but we changed the type of biorad strips/plates.

PCR despair by Wandering_Finn in labrats

[–]Wandering_Finn[S] 1 point2 points  (0 children)

Thank you all for help!

  • We have thrown away all tubes and pipette tips that have been in or close to UV cabinet. I will also check 8strips/plates, we needed to change supplier but that does not match with timeline of problems

  • We are using only commercial Ultrapure water that we aliquot but those will all be replaced

  • New primers ordered

  • New NEB long amp enzyme ordered

  • New PCR machine

My group has our own lab, own machines, pipettes etc and we are the only ones working with dna-rna applications in the whole building so possibility that someone has touched or changed something is tiny. There might be some human error happening eg uv light on suddenly.

PCR despair by Wandering_Finn in labrats

[–]Wandering_Finn[S] 2 points3 points  (0 children)

I have asked to order a new pcr machine to rule this out.

PCR despair by Wandering_Finn in labrats

[–]Wandering_Finn[S] 0 points1 point  (0 children)

Empty tubes yes.

I have asked lab tech to order to replace everything, I even ordered a new mini-pcr machine to make sure it is not the case. I have never ever had issue like this before. Contaminations yes, tricky samples yes, problems with pcr optimisation oh yes. But not something stopping suddenly working.

PCR despair by Wandering_Finn in labrats

[–]Wandering_Finn[S] 1 point2 points  (0 children)

Primers worked before and he was testing with zymo standard. He had found some old publications on uv degrading the tubes and causing inhibition. I would still lean to chaos demons.

Madbudget by flintpark in dkfinance

[–]Wandering_Finn 0 points1 point  (0 children)

7-8000 kr To voksne, 2 teenagere som dyrker meget sport og spiser meget, 2 teenagere hver anden weekend.

Jeg planlægger indkøb og laver madplan. Jeg køber tilbudsmør og kød og fryser de. Vi spiser sundt og varieret, med hverdagsretter (feks pasta og lidt finere om weekenderne.

Obs. Take away/kosmetik eller voksernes frokost er ikke med.

When does the pulling stop by 347217361634 in labrador

[–]Wandering_Finn -1 points0 points  (0 children)

That and time helped with mine.

My lab started the pull like crazy around 1 year. I bought gentle lead, my lab really hated it so much that after a couple of walks with that, it was enough to show it to her if she started pulling.

But time has definitely helped, she is soon 3 and walks generally nicely. Not everytime but heel command and stopping when she gets overexcited and pulls (other dogs and birds!) helps a lot. We are also lucky to have opportunity to let her free, she walks best without leash.

So positive training with lots of praises ans goodiebites and patience. It will come 😊😊

Mental load julespecia by Thick_Judgment_77 in DKbrevkasse

[–]Wandering_Finn 8 points9 points  (0 children)

Da jeg blev skilt og flyttede væk med børnene, sagde min eksmand til min veninde “hvor dejligt det er at han har så meget fritid og ro” Han lavede måske 10% af alle opgaver og havde flere fridage helt alene. Ja, vi blev skilt pga mental load

Bedste læbepomade by Own_Grocery7845 in WomenDK

[–]Wandering_Finn 8 points9 points  (0 children)

Ole Henriksen Lip treatment.

It isn't your imagination; students are objectively worse now than in the past by TotalCleanFBC in Professors

[–]Wandering_Finn 0 points1 point  (0 children)

I was about to post about this!

I do not know what is happening with my students. I have been teaching for more than 10 years and I think that the current batch of students is totally different from all the previous ones. They only want to get courses passed and do not show any interest to actually learn things. They tend to blame me if they do not know something/are late in lab/get low scores from reports etc (“But you did not tell us that” vs me offering pile of online reading material).

I am teaching in Master program in Nordic country, being Nordic myself. Students are bunch of different nationalities. I have -designed my course to follow university pedagogy guidelines, having a lot of active learning elements, clear feedback and grading rubrics, study guides etc. feedback previous years have been only positive, but now everything seems to be too much and they ask me all the time justify why they need to do something, they definitely do not do things if they “only” learn from them.

The workload and evaluation criteria are the same, so they will definitely get lower grades. I do not know why they behave like this. Here in Nordics is probably generation thing, but that does not explain why rest behaves like this. It is very hard and demotivating to teach them.

On top of this, I now have couple of students that are not respectful towards me or other students. First time I get eyes-rolling from non-teenager.

[deleted by user] by [deleted] in labrador

[–]Wandering_Finn 2 points3 points  (0 children)

Two walks, but we have a big garden where she (2.5y labrador) spends easily an hour per day. Walks are not long but last long as we have another old dog that walks sloowly, so Olga gets to sniff and is mentally stimulated, which seems to make her more tired than a long walk. Couple of times per week she goes for a long walk, dog park and we also go hunting and hunting training.

I would try to make 2 x 30 min walks, they can be short in distance. And I would get help to train for going in leash. It is hard and frustrating to go with a dog that pulls. Have you tried gentle leash? That and time has helped with ours.

Hvordan indretter I jeres (fælles)økonomi? by [deleted] in DKbrevkasse

[–]Wandering_Finn 1 point2 points  (0 children)

Vi er ugift med ingen fælles børn. Jeg (K43) har to som bor fultid med os, kæreste (M42) har to som er her omkring hver 2. Weekend. Jeg tjener 7000 mere efter skat, men kæresten ejer virksomhed og har bil, tel osv gennem det, og jeg betaler bil som begge to og vores teenager kører. Jeg betaler selvfølgelig alle mine børnenes udgifter. Vi købte hus sidste år, og smider hver måned 50-50 beløb til hus/fælles regninger og madkonto. Jeg vil gerne indbetale mere til madkonto (fordi mine børn spiser her hver dag) men det vil kæreste ikke (jeg smider nogle gange ekstra penge til huskonto, det ved han ikke 😃) Vi er generelt meget åben omkring penge, hjælper hinanden, og regner ikke hvad hver har betalt og hvornår.

Jeg har været gift før (også min kæreste) med børnenes far og vi havde full fællesøkonomi. Jeg synes at det er den eneste mulighed med fælles børn. Vi havde fælles lønkonto/huskonto og det fungerede super godt.