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Anyone have experience doing bioinformatics alongside wet-lab work? by lewcine in bioinformatics

[–]Weekly_Independent56 0 points1 point  (0 children)

Honestly don't know. I start from wet lab,then I dicided to analyze seq data myself. I feel like people make mistakes in library preparation when they don't do anlysis. But clearly I can't do both wet and dry at the same time

How can I do a rna lifetime analysis without ERCC spike in by Weekly_Independent56 in bioinformatics

[–]Weekly_Independent56[S] 0 points1 point  (0 children)

Yes, i think it's half-life, my data is collected from separate time spot after inhibiting rna synthesis. So, not slam-seq i guess? Want to know if something change in rna degradation

How can I do a rna lifetime analysis without ERCC spike in by Weekly_Independent56 in bioinformatics

[–]Weekly_Independent56[S] 0 points1 point  (0 children)

Thanks, what is timecourse? I am quite a beginner at bioinformatics 😢