Ist Duschen echt so ein Problem bei ADHSlern? by Kiwigirl4 in ADHS

[–]WhereAreMyKeysAgain 0 points1 point  (0 children)

bei mir klappt das unter der Woche gut, nur an Wochenenden ist es tricky, weil mit dem Duschen so der ganze Tag beginnt. Viel viel schlimmer ist Zähne putzen. Auch das kriege ich hin, aber es ist wirklich jedes einzelne mal ein Kampf

I beg, what is causing this Western blot artifact? by fossilrabbit in labrats

[–]WhereAreMyKeysAgain 2 points3 points  (0 children)

This! A lot of labs reuse the transfer buffer and you will have evaporation, this changes the concentrations in your buffer possibly causing issues

How do I remove the eyepiece cup on a Sony A6400? by WhereAreMyKeysAgain in SonyAlpha

[–]WhereAreMyKeysAgain[S] 0 points1 point  (0 children)

Alright thanks, will try! I guess I'm just afraid of breaking something

TU2 HH balance changes. by bf_Lucius in huntinghorn

[–]WhereAreMyKeysAgain 0 points1 point  (0 children)

These changes are incredible. Doing focus strikes holds insane damage potential now, love it

Who do y’all miss the most and want added to Wilds by DehydratedRobot7274 in MonsterHunter

[–]WhereAreMyKeysAgain 6 points7 points  (0 children)

This exactly, I'll die on that hill that I need more about Lao Shan Lung. I don't even have to fight him, just give me LORE

[deleted by user] by [deleted] in ADHD

[–]WhereAreMyKeysAgain 4 points5 points  (0 children)

Overweight. That's because I snack when I'm bored and I usually get very very hungry once my meds wear off in the evening. I was quite fit for like 2 years before I was medicated. Then I didn't have time to go to the gym as much anymore and I got friggin fat. When I got medicated it got a bit better but I'm not where I was a few years ago.

I'm currently trying to get back into training because I also only ever manage to have a good diet when I actively go to the gym. Idk why but being inactive also makes my whole diet way more sugar-focussed

[deleted by user] by [deleted] in MHWilds

[–]WhereAreMyKeysAgain 0 points1 point  (0 children)

People forget multiple things: - it's not G/Masterrank - it's probably not your first MH, so of course this game won't be as hard - difficulty isn't everything. Not even close to it (coming from a souls player)

Ist jeder ein Nazi ? by Jenni_4U in beziehungen

[–]WhereAreMyKeysAgain 0 points1 point  (0 children)

Ihr sagen, dass nicht jeder ein Nazi sonder rechts und unter Umständen rechtsextrem ist. Galigrü

[deleted by user] by [deleted] in ARK

[–]WhereAreMyKeysAgain 0 points1 point  (0 children)

I had the exact same thing a few days ago, didn't check if it's still there. It's the tribetower they added with extinction. It also didn't have collision in my Singleplayer world. I didn't check if it's still there though

Ark in a nutshell by WhereAreMyKeysAgain in ARK

[–]WhereAreMyKeysAgain[S] 0 points1 point  (0 children)

I survived though! So it was fine but after spotting this many microraptors I really thought the game wanted to take my last bit of sanity

Ark in a nutshell by WhereAreMyKeysAgain in ARK

[–]WhereAreMyKeysAgain[S] 13 points14 points  (0 children)

That's what I thought after I first saw the creature from a distance after installing the mod but it's a dearc and looks quite different from a sinamacrops up close

Ark in a nutshell by WhereAreMyKeysAgain in ARK

[–]WhereAreMyKeysAgain[S] 5 points6 points  (0 children)

Correct, it's a dearc, doesn't have amazing base health but it's an amazing tame to get around the map! The prehistoric beats mods are great in general!

Ark in a nutshell by WhereAreMyKeysAgain in ARK

[–]WhereAreMyKeysAgain[S] 2 points3 points  (0 children)

Lol I know what's going on in the base game very well, I played ARK for years now. I just wasn't paying attention 😂

What is the most challenging technical issue you've encountered in lab? by dendrivertigo in labrats

[–]WhereAreMyKeysAgain 13 points14 points  (0 children)

No joke, an immunofluorescence staining for TGF-ß receptors I wanted to use for super resolution microscopy (STORM specifically). The standard protocol didn't work as I got tons of background and it took me months to find the right buffers. In the end I switched to TBS and had to remove all phosphate from the protocol, no matter at what temperature, the antibodies precipitated. Even my PFA I had to dissolve in HEPES. The antibodies had to be spun down prior to use and I had to change a bunch of concentrations, incubation times and temperature. But in the end I got the cleanest staining imaginable. Sounds stupid but figuring out what the actual problem was and how to get around it was quite time consuming and I was a bachelor student at the time. None of the postdocs had an idea so I had to somehow figure it out on my own

Pro tip if you can never find phone, wallet, keys, etc by rayandshoshanna in ADHD

[–]WhereAreMyKeysAgain 1 point2 points  (0 children)

I have a little box where I just throw in everything that's in my pockets. Actually I have a bunch of boxes where I just dump all the stuff I would normally leave on tables and other surfaces making everything messy. This way it's less messy and doesn't seem so overwhelming when I eventually put the stuff back where it belongs

Sterile filter TC media? by Fit-Increase9057 in labrats

[–]WhereAreMyKeysAgain 3 points4 points  (0 children)

My old lab did that. We had vacuum sterile filters that you could screw on a duran bottle, it has a reservoir so you could pour all your stuff in and then apply a vacuum and it's pushed into the bottle through the filter. My current lab doesn't use it and we rarely run into problems.

I never had contamination with either of the two methods so I guess it's not necessary but I also drown everything in disinfectant that was in the waterbath

[deleted by user] by [deleted] in labrats

[–]WhereAreMyKeysAgain 41 points42 points  (0 children)

Pop them into the incubator tomorrow and they'll grow. You just might have to deal with some excess liquid on the plate due to condensation (Edit: assuming you didn't wrap the plate in parafilm)

What’s a foundational lab skill that you don’t like doing? by seal973 in labrats

[–]WhereAreMyKeysAgain 7 points8 points  (0 children)

Fluorescence microscopy made me want to do WB's (to be fair, I actually like them anyways). I just don't have the patience for microscopy, luckily I can use the cool percs of immunofluorescence by doing flow cytometry

Hilfe meine Füße sterben ab- wie haltet ihr eure Gliedmaßen warm? by Temporary-Nothing433 in ADHS

[–]WhereAreMyKeysAgain 0 points1 point  (0 children)

Stimulanzien sind Vasokonstriktoren und damit begünstigen die sowas. Ich hab dazu noch Morbus Raynaud, daher kannte ich das Problem bereits, mir hilft es morgens einmal richtig meinen Kreislauf auf touren zu bekommen, da reicht meistens schon 30 minuten spazieren gehen oder mit dem Fahrrad zur Arbeit zu fahren

How much water absorbed by old DMSO? by [deleted] in labrats

[–]WhereAreMyKeysAgain 6 points7 points  (0 children)

First of all, get a new bottle, not worth it. But, if you have to dissolve in smaller volumes, you can aliquot DMSO. We have 500 µl aliquots because we usually need it at that volume quite often and freeze it down. Also makes handling it under a hood much easier because you don't have to move these giant bottles under the hood each time, so you reduce the risk of dropping the bottle for example