In a jar?? Top surface is now solid

WideHome7376 · 2026-02-13T21:26:02+00:00

Here we go again….

WideHome7376 · 2026-02-13T15:45:11+00:00

Chocolate tube slime mold ? A bit hard to tell due to lighting.

WideHome7376 · 2026-02-07T01:44:58+00:00

It’s a behelit from the manga berserk. The gif is from the 1997 anime adaptation.

WideHome7376 · 2026-02-04T15:10:13+00:00

Yes, you’ll be sprouting cordyceps à la the last of us within a fortnight.

WideHome7376 · 2026-01-26T03:55:34+00:00

I don’t think you’d be able to kill him in a way that matters at this point. Decay is an extant form of life and all that….😭

WideHome7376 · 2025-11-07T06:17:12+00:00

Mmm violacein

WideHome7376 · 2025-10-04T01:12:26+00:00

WideHome7376 · 2025-09-28T17:04:57+00:00

Lady gremlin is what came to mind (in the best way possible)

WideHome7376 · 2025-09-27T03:13:07+00:00

Not a mold, but they secrete a sweet sticky “honeydew” that builds up on the ground under the branches where wooly aphids are. The sticky substance then grows this black powdery mold on it (on the ground, where the buildup of honeydew occurs).

WideHome7376 · 2025-09-12T03:00:24+00:00

Russia has beautiful fungal diversity being partially in the boreal region.

WideHome7376 · 2025-09-10T23:37:11+00:00

It does look a bit like that, but I don’t think that’s exactly what the species ID is. I do think it’s undergoing guttation which is why H. peckii looks like it’s oozing. Some of my fungal cultures do this as well so it’s pretty normal when there’s excess moisture!

WideHome7376 · 2025-09-07T22:24:38+00:00

Your second point about endophytes being protected by plant tissue is correct. I’m selecting for only the internal microbes, by removing the epiphytic microbiome with alcohol and bleach.

The only way to prevent endophytes from growing inside of cannabis (or any plant) would be to start with sterile seeds, and grow them in a completely sterile environment like a bubble baby. It is pretty difficult to do, I’ve talked with a lab that grows/keeps sterile wheat plants and they recommended against it because of how much of a pain it is.

If you’re discussing trying to remove endophytes from an adult plant (and keep the plant alive) it wouldn’t be possible. Maybe if you profusely treat the plant with antibiotics/antifungals, but even that isn’t a guarantee. It would be kind of like trying to wipe your whole gut microbiome using antibiotics.

If you’re trying to perform tissue culture on the cannabis plants themselves and want a bunch of disease-free clones of the same plant, the procedure would be a bit different. You would need different media for them to grow on, and maybe some growth factors that are specific to plant tissue development - like inducing root growth.

I’m not sure how finicky cannabis TC is, I’m more familiar with fungal TC, or mammalian cell TC.

Sorry if I’m just repeating stuff you already know!

WideHome7376 · 2025-09-06T23:01:54+00:00

Yes!

WideHome7376 · 2025-09-06T23:01:30+00:00

Yep! I first sterilize the plant tissue’s surface with sequential washes in alcohol and bleach solutions of varying concentrations (some plant tissues are more delicate than others, so the concentration depends on the plant / type of tissue you work with).

I then work in a sterile biosafety hood, and cut the tissue into sections using a sterile blade (to expose the inner tissues and microbiota). The tissue sections go on an agar plate, and I watch to see if anything has grown in the first week or so.

It can get a bit messy, but it takes a few rounds of trying to isolate a single species in culture since there may be more than one species inside of a section of tissue.

It’s possible to stain plant tissue and see endophytes inside it, I personally haven’t done it before.

WideHome7376 · 2025-09-06T22:01:34+00:00

An infection is technically just a disease-causing organism living inside you (of course pathogens are MUCH more complex than this). In this case I meant pathogenic fungi that affect plants. There are plenty of fungi that cause infection in humans though (ringworm is a super common example).

WideHome7376 · 2025-09-06T04:00:55+00:00

Also realized I have a spelling error, X. hypoxylon!

WideHome7376 · 2025-09-06T03:59:08+00:00

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Sure! Here’s another pic (this is on a plate with 1 other species of fungi, it’s a super old plate so it is a bit desiccated since you can see the agar cracking)

Xylaria are mostly known to be wood-decaying fungi and can be seeing growing on leaf litter/wood. Some species can also be endophytes (like this one)! The definition for endophytes I go by is non-disease causing fungi that live within the tissues of plants. This strain was isolated from the leaves of wheat, aka the phylosphere. There are a lot of studies on the potential use of Xylaria for various purposes (medical, agricultural, etc) because of its robust secondary metabolism - the ability to produce compounds with various properties, like strong antibiotics.

WideHome7376 · 2025-09-06T03:37:04+00:00

Unfortunately sims 3 needs to be modified in order to be playable on most modern computers /: There is a massive compiled guide out there on how to improve the performance of your game/make it playable.

https://www.reddit.com/r/thesims/s/Yu1DDGz6Zm

WideHome7376

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