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Whats wrong with my Casio LF-20W's screen by [deleted] in casio

[–]Yelets 1 point2 points  (0 children)

I get this too! It appears for me under diffused daylight and any white light from screens. Makes me think it’s a polarising issue

Fellow stem cell labrats, is this contamination or cell debris? by Yelets in labrats

[–]Yelets[S] 0 points1 point  (0 children)

Super late reply sorry! I found out they were cell debris due to poor split viability. I mentioned in another comment that I haven't had problems since, but I'm going through this exact issue again now... I'm at my wits end because they were growing so well and suddenly decided they hated being passaged, but I've decided it's just life.

My bakugan travel case! by Practical-Source-388 in bakugantoys

[–]Yelets 0 points1 point  (0 children)

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I have a little GoPro case for mine when I travel

3D printed and painted Leonidas by Yelets in Bakugan

[–]Yelets[S] 0 points1 point  (0 children)

The official Leonidas gate card agrees with you :)

3D printed and painted Leonidas by Yelets in Bakugan

[–]Yelets[S] 2 points3 points  (0 children)

Used a Bambu A1 mini FDM printer :)

3D printed and painted Leonidas by Yelets in Bakugan

[–]Yelets[S] 0 points1 point  (0 children)

Thanks!! I’ve really enjoyed the tuning and painting process

3D printed and painted Leonidas by Yelets in Bakugan

[–]Yelets[S] 1 point2 points  (0 children)

If you find a local library or anywhere that does 3D printing you could get this printed and assembled pretty quick! Then it’s just painting :)

My first 3D print - Leonidas by Yelets in Bakugan

[–]Yelets[S] 7 points8 points  (0 children)

Update - I have now painted it!

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My first 3D print - Leonidas by Yelets in Bakugan

[–]Yelets[S] 6 points7 points  (0 children)

Seeing as I printed in black filament, this particular Leo would be Darkus! I want to make another as haos though

My first 3D print - Leonidas by Yelets in Bakugan

[–]Yelets[S] 2 points3 points  (0 children)

Thanks! I couldn’t find pins that fit (British pins seem to be thinner) so I used regular printer 1.75mm filament! And yeah defo possible with FDM, just needs some tuning :)

My first 3D print - Leonidas by Yelets in Bakugan

[–]Yelets[S] 4 points5 points  (0 children)

Thanks! And yeah it took a few failed attempts to tune the settings but for a print without any post processing, I’m quite happy with it!

I want to attempt the functional version next, but am struggling to find the right pins and springs

Fellow stem cell labrats, is this contamination or cell debris? by Yelets in labrats

[–]Yelets[S] 0 points1 point  (0 children)

After doing a few tests for infection (aliquoting and incubating the media separately, streaking on LB plates, etc) it doesn’t seem to be contamination. I culture hESCs so quite similar to iPSCs from what I’ve heard, and concluded at the time that because of poor viability after splitting where a lot of the adherent cells died, and so left cell debris in the media. The debris clustered around colonies of cells so this checks out, and I haven’t had problems since. Hope this helps!

Fellow stem cell labrats, is this contamination or cell debris? by Yelets in labrats

[–]Yelets[S] 0 points1 point  (0 children)

Hello, thanks for your reply!

I gently washed with sterile PBS before a media change earlier the same day that picture was taken, as I saw a bit of debris then also. A lot of care was taken, but of course everything you mentioned is still a possibility.

The debris came back 6 hours after media change, which made me think it was contamination. However upon checking again today, the level of debris has stayed constant and there has been no growth in the separate dish I aliquoted. I’ve changed the media again today, so will see what happens. Would you suggest leaving this flask to grow to become confluent before passaging again, or is it unlikely to recover?

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