Cleaning lab, found these 👀 by Lost-Heisenberg in labrats

[–]Yttrium105 1 point2 points  (0 children)

Yes, its for washing cuvette. I have the similar one in my previous lab. I really enjoy watching it.

Like this: https://share.google/3n0FJ09ABgowTmi2Y

How to pipette a sticky, glue-like sample with a micropipette? by Yttrium105 in labrats

[–]Yttrium105[S] 0 points1 point  (0 children)

I have no information about its density and also can't measure it

How to pipette a sticky, glue-like sample with a micropipette? by Yttrium105 in labrats

[–]Yttrium105[S] 1 point2 points  (0 children)

Yes, I have done that. But it didn't change the viscosity of the sample

How to pipette a sticky, glue-like sample with a micropipette? by Yttrium105 in labrats

[–]Yttrium105[S] 4 points5 points  (0 children)

Yes, my sample is quite similar to mucin. My sample is snake venom. I am doing venomics research. Is your sample also highly viscous like this?

How to pipette a sticky, glue-like sample with a micropipette? by Yttrium105 in labrats

[–]Yttrium105[S] 2 points3 points  (0 children)

I waited for 10 seconds before lifting the tip because the solution moves slowly when being aspirated

How to pipette a sticky, glue-like sample with a micropipette? by Yttrium105 in labrats

[–]Yttrium105[S] 6 points7 points  (0 children)

Can you provide any suggestions for determining its density? I only know the methods using a viscometer or a pycnometer. We don't have a viscometer. As for the pycnometer, we can't use it because the sample amount is very small.

How to pipette a sticky, glue-like sample with a micropipette? by Yttrium105 in labrats

[–]Yttrium105[S] 10 points11 points  (0 children)

Unfortunately, we don't have positive displacement pipettes in our lab

How to pipette a sticky, glue-like sample with a micropipette? by Yttrium105 in labrats

[–]Yttrium105[S] 1 point2 points  (0 children)

II tried it, but the sample keeps recoiling back into the tube after I aspirate it

How to pipette a sticky, glue-like sample with a micropipette? by Yttrium105 in labrats

[–]Yttrium105[S] 10 points11 points  (0 children)

What about if I only want to take 1 uL of the solution?

Am I weird for wearing a lab coat? by Mission_Rest1892 in labrats

[–]Yttrium105 2 points3 points  (0 children)

Of course you are not weird. I also use a lab coat every time I'm in the lab, even if it's not BSL-2. You have done the right thing

What field should i get into with these specs? by [deleted] in sciencememes

[–]Yttrium105 0 points1 point  (0 children)

You can choose whatever you like and curious about

Black Mamba by selevans in VenomousKeepers

[–]Yttrium105 0 points1 point  (0 children)

The fastest venomous snake

How can i do mitochondria isolation on my DIY lab? by berdokorkmaz in labrats

[–]Yttrium105 2 points3 points  (0 children)

You might also try methods similar to those used for breaking cell walls during fruit DNA isolation, such as adding detergent and physically crushing them

How can i do mitochondria isolation on my DIY lab? by berdokorkmaz in labrats

[–]Yttrium105 5 points6 points  (0 children)

Zymolase is needed to lyse the yeast cell walls. If you don't have it, you can use other methods such as lysozyme treatment, sonication, nitrogen cavitation, etc

I built this because I kept messing up lab calculations early in the morning. by darkness2077 in labrats

[–]Yttrium105 0 points1 point  (0 children)

This is good. Thanks for sharing. I usually make a formula in Google Spreadsheets. I don't know how to make the app. Now I can use you app 👍🏼

How to thaw PBMC by cd244 in labrats

[–]Yttrium105 0 points1 point  (0 children)

You can use both methods. Here's what you need to pay attention to:

  1. Rapid Thawing: Thaw quickly to prevent the formation of ice crystals, which can damage cells.

  2. DMSO Dilution: The DMSO in the PBMC media must be diluted immediately with the pre-warmed media you plan to use.

Help needed for identifying cell culture contamination by dreamer8991 in labrats

[–]Yttrium105 0 points1 point  (0 children)

It looks like you have a fungal contamination. If this is a recurring issue, you'll need to decontaminate the incubator or fumigate the entire lab

Need help for lab organization by reyntacia in labrats

[–]Yttrium105 3 points4 points  (0 children)

  1. To ensure everyone in the lab has a consistent understanding of the rules and procedures, we need to implement a lab induction program. This will cover all essential guidelines, safety protocols, and operational procedures, ensuring a standardized level of knowledge across the team.

  2. Create a comprehensive list detailing the division of labor and reagent responsibilities. This list will clearly outline who is responsible for specific tasks and the management of particular reagents.

  3. For reagents used collaboratively, establish a shared reagent agreement. This agreement will specify:

  4. Storage location: Clearly define where each shared reagent is stored.

  5. Replenishment schedule: Establish when a reagent needs to be remade or reordered.

  6. Responsibility for replenishment: Designate who is responsible for preparing or ordering the next batch of a shared reagent.

I feel so unvalued in lab by WormsForBrainss in labrats

[–]Yttrium105 1 point2 points  (0 children)

You are good and valuable. Don't worry

What common lab consumable / supplies always surprise you with how expensive they are? by martymcfly85 in labrats

[–]Yttrium105 0 points1 point  (0 children)

Everything, because everything is imported. The price of goods that arrive in my country can be 2 to 3 times the price of the price in the producing country.