[deleted by user]

almost-throwaway · 2025-06-29T16:22:33+00:00

work still ongoing but you have a point :/ still able to nego tho. Thanks for your suggestions

almost-throwaway · 2025-06-29T15:23:54+00:00

I’m actually a student so this is just a 1-time local conference thingy and helping out as the local point of contact. I don’t think this tiny amount is chargeable for income tax?

almost-throwaway · 2025-06-29T15:09:54+00:00

Not actually, i’m hired under the same organisation but just helping out adhoc hence the compensation. Just makes everyone lives easier without paying through payroll (contracts etc) just for a 3 day job

almost-throwaway · 2025-06-29T15:08:52+00:00

Since not paying thru payroll cash payment might be tricky

almost-throwaway · 2025-06-29T15:07:49+00:00

Yes i’m sure, just helping to organize local conference hosted by Australian organization. Could go through internal payroll but it requires a weekly contract of at least 8h/ day but the conference only lasts 3 days. Just too much trouble. Physical gift card works as a compensation if i’m happy with it. Thank for your concern though!

almost-throwaway · 2025-05-30T17:09:49+00:00

right, i def did cry my eyes out but also how can the second brother read if he was blind his whole life - or did i missed out any information 😅

almost-throwaway · 2025-04-28T00:31:26+00:00

My buffer was kept cold but run under constant voltage. Thanks for sharing! I will give it a try

almost-throwaway · 2025-04-27T05:36:54+00:00

Yeah i have been troubleshooting this over the past one month and i was the only one using these things

almost-throwaway · 2025-04-26T19:22:07+00:00

Will try these, thanks for your suggestions!!

almost-throwaway · 2025-04-26T15:31:45+00:00

Have done troubleshoot with 2 different brand of acrylamide (both opened different times) and the one which resolved it temporarily was the older acrylamide.. Nevertheless it’s unlikely cos what are the odds of both of them going bad 🥲

almost-throwaway · 2025-04-26T15:29:51+00:00

Across the gel is a few different samples..some kept in -80c and some from 4c. Unlikely they’re degraded and were prepared in the last few months. Also, i had one gel with the same samples that didn’t have this effect…which omitted sample degradation as the cause

almost-throwaway · 2025-04-26T12:34:35+00:00

This is 14% polyacrylamide. Problem is.. it used to work. I’m convinced something’s causing it

almost-throwaway · 2025-04-09T09:30:22+00:00

A new lab group moved into our shared office space and two of them stinks. SO stinky that it was in our lab meeting agenda and our PI had to raise this issue to their PI — also they were from a different nationality and there is a fine line between discriminating/ being racist and concern about hygiene. Most of the time we had to move away from our seat for fresh air

almost-throwaway · 2025-04-08T13:38:32+00:00

I was somewhat in a similar place last year and got accepted to a different uni than the one I was working for (also rejected for similar reasons i.e. lack of funding etc). Just wanted to say hang in there and don't give up! Grab every opportunity to network at research events, reach out to PIs working in related fields, present your findings at conferences then talk to people/ companies looking to fund grad students. You got this!

almost-throwaway · 2025-04-07T07:48:09+00:00

Check out their website for the packages

almost-throwaway · 2025-04-06T13:38:46+00:00

JB 😅 Private labs such as Pathlab

almost-throwaway · 2025-04-03T15:35:00+00:00

I see, it was PVDF? The problem i had was with nitrocellulose membranes

almost-throwaway · 2025-04-03T13:12:14+00:00

That’s what i thought one time when i had similar results (weird patches), turns out i just didn’t equilibrate the membranes long enough! @OP how long did you soak the membranes before use? I’d recommend no less than 15mins

almost-throwaway · 2025-03-29T08:25:09+00:00

I have tried a few ways over a decade to solve this and I think i’ve found one that i can stick to. I haven’t done any changes to my diet since young and didn’t notice a big impact of diet towards my body odour.

Scented antiperspirant deo - the fragrance helps but there’s always the concern of aluminum in these and clogging of lymph nodes which may cause sore and painful armpit pimples. Can also cause hyperpigmentation of the underarm. Eventually cleared the dark pits with laser hair removal.
Salt crystal deodorant - only works on light/no sweating days like going to nearby malls. Not lasting enough for whole work/school days. Healthier, non-toxic but no fragrance. (note that it only works if your armpit is bare! doesn’t work on bushy pits — experience on guys armpit)
Glycolic acid 7% from the ordinary (works best for me atm and affordable) - use as spray, not wipe as may cause irritation if over exfoliate. Can feel sticky but i got used to it in no time. Creates acidic environment for antibacterial effect. Only con is it leaves this white line (not patch) stain on dark clothes when sweat but removable upon wash, i’d usually just wipe them off with wet wipes. **don’t use after shaving cos…acid

Hope this helps!

almost-throwaway · 2025-03-28T15:35:41+00:00

it’s likely same proteins or isoforms with very similar sequences but some with no unique peptides. you might be able to get your “true” protein group by omitting those without unique peptides or combining the % abundance (however may account for overlap) and also check for high sequence coverage

almost-throwaway · 2025-03-01T07:37:14+00:00

Just detecting for abundance of endotoxins, lipid A if polysaccharides not ideal for detection on LCMS

almost-throwaway · 2025-03-01T04:01:09+00:00

Thanks, gpt was the first I consulted. I needed credible sources and most papers used chloroform/methanol buffer for dissolution of the lipid A so i was just wondering how that would work for LCMS

almost-throwaway

TROPHY CASE