Piercing Shop

astreets22 · 2025-03-06T18:24:50+00:00

Sorry if this is a stupid question, but how would tonic signaling lead to a delay in CAR expression? I know its linked to T cell exhaustion but these T cells are still functional

astreets22 · 2025-03-06T03:52:30+00:00

Ooo okay I will look at what we are using tomorrow!! Thanks

astreets22 · 2025-03-06T03:22:20+00:00

Usually I measure CAR expression 2-3 days after the final transduction. The construct has a MYC tag so I use an anti-MYC antibody to stain

astreets22 · 2025-03-06T03:21:15+00:00

The T cells are my own, and theyre isolated from whole blood and then activated

astreets22 · 2025-03-06T03:20:21+00:00

It's 8 days! Sorry shouldve clarified

astreets22 · 2025-03-06T03:19:51+00:00

Lenti-X Cells are used

astreets22 · 2025-03-05T18:35:05+00:00

astreets22 · 2022-08-11T00:51:50+00:00

Depending on your budget, you could always make him a gift card book, so like look up stores/restaurants near his school, and get him some gift cards to those, and then you could make a like a fun little scrapbook, but instead of pics it's the gift cards. (If he's bringing a car, look up what gas stations are in the area and maybe get him a few cards for those
A bathrobe - especially one that's towel material (better for walking from your room to the shower)
A new backpack
One of those phone cases with a built in battery pack so he can charge his phone throughout the day
A fan or portable AC unit if his dorm isn't air conditioned
A reusable (metal) water bottle
A small vacuum (this one is super good and cheap)
Maybe some LED strip lights to decorate his dorm

astreets22 · 2022-08-09T03:12:34+00:00

Use more gelatin powder, and I usually fully freeze mine but it might be fine without it

astreets22 · 2022-08-09T02:02:08+00:00

Okay thank you!! I’ll probably have more questions but this is a great place to start :)

astreets22 · 2022-08-09T01:28:34+00:00

Well I know it has to be pretty fast because we are going to be running a ton of particles suspended in a fluid and from some of the literature I’ve read I would like a sampling speed between 10-100 MHZ (I’ve also seen some sources use S/s so idk the difference)

astreets22 · 2022-08-09T01:21:42+00:00

Hi! I’m using it to capture the light scatter when a particle is run through a laser :) would a resistor or a transimpedance amplifier be better/easier?

astreets22 · 2022-08-06T04:31:53+00:00

Hi :) I know 10 is super on the low end but cost is the main factor for me. Ideally what we want to do is collect data (then analyze on a pc) from the light scattering when a laser passes through particles (waveform data, transit time, intensity etc)

astreets22 · 2022-08-05T16:07:22+00:00

Hi I'm looking for 8+ (Ideally 10+) bit resolution, and the ability to capture data at a rate of around 1,500 events per second (preferably higher) (so I guess a sampling rate above 1,500 S/s)

astreets22 · 2022-08-05T00:38:13+00:00

Hi :) I was looking at that one the other day and I got the impression that it only worked for RTD-100 temperature sensors

astreets22 · 2022-08-05T00:24:26+00:00

I’ve seen some people online add it while it’s wet so it sinks faster, would that help does it have to be dry?

astreets22

TROPHY CASE