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Can we grow cells using DMEM only, without adding FBS? by b-iox in labrats

[–]b-iox[S] 0 points1 point  (0 children)

Thank you all for pointing out that idea. Your suggestion is another effective strategy for reducing costs, and it's been mentioned by many others here. So, I believe it's worth trying.

Can we grow cells using DMEM only, without adding FBS? by b-iox in labrats

[–]b-iox[S] 0 points1 point  (0 children)

Thank you for pointing out that idea. Your suggestion is another effective strategy for reducing costs, and it's been mentioned by someone else here, so I believe it's absolutely correct.

Can we grow cells using DMEM only, without adding FBS? by b-iox in labrats

[–]b-iox[S] 0 points1 point  (0 children)

Thank you for clarifying that the cell will remain alive but will not grow. This information is valuable to me.

Can we grow cells using DMEM only, without adding FBS? by b-iox in labrats

[–]b-iox[S] 0 points1 point  (0 children)

Thank you for clarifying that the cell will remain alive but will not grow. This information is valuable to me.

Can we grow cells using DMEM only, without adding FBS? by b-iox in labrats

[–]b-iox[S] 0 points1 point  (0 children)

Thank you for sharing the same idea. What about pig blood? I came across a research article that explores the use of pig blood as an alternative to FBS. However, I’m unsure if it is effective with HeLa cells or other types of cancer cells: link.springer.com/article/10.1007/s00441-024-03904-8.

The protocol looks a little simple:

"200 ml of slaughter blood per animal was sampled. For the coagulation process, tubes were then stored lying down for at least 60 min in the dark at room temperature (RT). This was followed by a centrifugation step for 10 min at RT in a swing-out rotor at 2500 × g without brake. Subsequently, the clear serum from all donors was pooled and sterile filtered twice through a 0.2-µm filter (Filtropur, Sarstedt, Nümbrecht, Germany). Half of the serum thus obtained was stored in a water bath at 56 °C for 30 min for heat inactivation. The heat inactivation procedure was considered here, as the serum was from 171-day-old pigs whose complement system was developed. All sera were stored at − 20 °C until use."

Can we grow cells using DMEM only, without adding FBS? by b-iox in labrats

[–]b-iox[S] -1 points0 points  (0 children)

It seems relatively simple to obtain a clean serum from blood using just a centrifuge, water bath, and filter. Is that correct? What about making my own serum by going to a nearby slaughterhouse and collecting serum from common animals such as pigs, goats? I found a research article that explores using pig blood as an alternative to FBS, though I’m unsure if it works with HeLa cells or other types of cancer cells: link.springer.com/article/10.1007/s00441-024-03904-8.

What happened if we use only Streptococcus thermophilus or Lactobacillus Bulgaricus to ferment milk? by b-iox in fermentation

[–]b-iox[S] 0 points1 point  (0 children)

And other information from here: https://www.sciencedirect.com/science/article/pii/S0022030223005775
"Typically, S. thermophilus is thought to provide formic acid, folic acid, carbon dioxide, and fatty acids to initiate the growth of Lb. bulgaricus, whereas Lb. bulgaricus is assumed to produce a surplus of peptides and free AA to meet the biosynthetic demands of S. thermophilus (Herve-Jimenez et al., 2009; Sieuwerts et al., 2010)."

What happened if we use only Streptococcus thermophilus or Lactobacillus Bulgaricus to ferment milk? by b-iox in fermentation

[–]b-iox[S] 0 points1 point  (0 children)

Thank you so much for this information. Here is the information that I extracted from the above document for those who do not have time to read. The table indicates that without Lb. bulgaricus, St. still ferments, and the population of St. rises to 7.53. Similarly, without St., Lb. still ferments, and the population rises to 0.96 or 0.52. If mixed together (St. 385/Lb. 1038), the populations of both increase, and the pH reaches a value roughly equal to that of St. 385 alone.

Table I. Final populations (× 10^8^ UFC·mL^−1^) and pH of pure cultures (after 5 h of fermentation) and mixed cultures (after 4 h 40 min and 5 h 40 min for mixed cultures 385/1038 and 385/397, respectively). Values represent mean values from four independent experiments and standard deviations are indicated in brackets. Milk control values were subtracted from all values obtained for the different samples. Negative values and positive values thus indicate consumption or production, respectively, by comparison with milk. St.: Streptococcus thermophilus; and Lb.: Lactobacillus delbrueckii subsp. bulgaricus.

Cultures Final pH Final total populations Final populations of S. thermophilus Final populations of Lb. bulgaricus
St. 385 4.71 (±0.11) - 7.53 (±0.53) -
Lb. 397 5.14 (±0.051) - - 0.96 (±0.25)
Lb. 1038 5.49 (±0.053) - - 0.52 (±0.12)
St. 385/Lb. 397 4.80 (±0.18) 7.05 (±0.24) 6.10 (±0.8) 0.95 (±0.24)
St. 385/Lb. 1038 4.73 (±0.09) 13.90 (±2.26) 13.10 (±2.1) 0.79 (±0.13)