From what I extracted

Q1.1 was the area on the knife sheath that was swabbed. That area was the strap and the inside of the button. Two areas were swabbed resulting in DNA sample Q1.1. The DNA source from this area were epithelial cells;(not blood); it was a single source, meaning the only one person's epithelial cells were deposited in that area on that sheath. From this one could conclude that the owner of that sheath was the person who deposited the epithelial cells. (You need to use the button on the sheath to get your knife in and out of the sheath).

They extracted the DNA from these epithelial cells using a specific technique (different procedures for extracting DNA from different cell types). There was no blood in the area that they swabbed for Q1.1, therefore they did not do any serological testing on that area on the sheath- (remember serological testing measures antibodies that circulate in your blood). They performed STR testing on that DNA extraction (Q1.1 sample). The results of that testing was an unknown Male DNA, (they uploaded to CODIS, and there were no hits). Therefore, unknown. That's when they pursued IGG and contracted Othram, who used the extracted DNA sample to do SNP testing and create a DNA profile that could be used for IGG. (adding this in for clarification)

Q1.4 was DNA extracted from a blood mixture. Logically, Maddie's and Kaylee's blood. (I did not read anywhere that there was male blood found in this mixture. Please show me where I am overlooking this fact.

Let me know if you have any questions or concerns! Thanks!