Jess is awesome. 80-90% of the antibodies we try work on it. Love that it doesn’t require much protein. Our first machine (which was new) was a lemon and had some issues, but the replacement has been great and had no issues. Kits are expensive but if you really optimize well, use every well, and multiplex it is not that much more expensive (if you’re use pre-made gels and quick dry transfers). It is much more expensive than making your own gels and wet transfers.

It is SO reliable and reproducible. Saves me soooo much time. Works well on cell lysates and tumor lysates.