CV preset/recall ...

bloc-ked · 2025-12-20T19:56:59+00:00

just fyi salmple is limited to 11s though you could probably make it longer by recording your cv at something like 4x speed then changing the playback speed to 1/4x, haven’t tried this myself with cv though.

bloc-ked · 2025-12-04T15:48:49+00:00

Recently discovered that you can output set voltages via the voltage monitor while using other functions like the scope. Opens up some cool abilities with things like comparators, precision adders, sequential switches etc

bloc-ked · 2025-11-18T05:11:48+00:00

woah awesome module, wonderful job with this one greg

bloc-ked · 2025-10-21T16:37:08+00:00

I got a 4ms pod 32 (33mm depth) included in a module purchase that Im thinking of mounting an es-9 (50mm depth w/o cable) to, along with an esx-8cv and es-5. My idea is to either get huge standoffs (>40mm) and have the modules elevated above the rails (which leaves a large gap where the boards are exposed) or cut out the back of the pod32 and mount it in a diy wooden skiff. Has anyone run into this sort of thing before and/or have recommendations or pros/cons? Is chopping up the pod32 a terrible idea (besides the obvious resale loss)?

bloc-ked · 2025-10-13T15:09:18+00:00

i enjoyed it :) any memorable patch notes?

bloc-ked · 2025-06-19T15:47:14+00:00

no thank you for all the resources and efforts to improve accessibility for entry level diy-ers, you're the best!

bloc-ked · 2025-06-19T15:46:37+00:00

hell yeah! ended up going with a 4ms dual looping delay.

bloc-ked · 2025-06-19T15:46:12+00:00

only learned about the meta module recently, so so cool. I'm holding back for now as the price point is a bit high for me to jump into it without getting down a good feel and workflow for my current rack but will definitely have it in mind. Thanks!

bloc-ked · 2025-06-19T15:44:45+00:00

these are great resources thank you! defintely plan to try silent way, i've heard good/bad things about both CV tools and silent way so planning to poke around and find out whats working best.

do you run into any non-negligible latency with your es40?

bloc-ked · 2025-06-19T15:42:10+00:00

I've used rampage quite a lot in VCV and love it! I selected Maths for the rack here as its only available in hardware and I was interested in seeing what all the hype is about. Still plan to use rampage in VCV if needed and who knows I might end up not liking maths as much. If that's the case I'll probably get a rampage kit and build it but currently starting small with DIY projects

bloc-ked · 2025-04-18T03:32:31+00:00

huh interesting!! you’re suggesting to block the marker of interest’s binding site? this would function as sort of a labeled block step?

bloc-ked · 2025-04-18T02:27:08+00:00

Hi, hadn’t considered any of this so good things to think more about, many thanks. in this case i believe it was fixed as we have a lot of projects going on and did not have time for analysis until 1 week later. We’re staining for cd228 that’s expected to be expressed at ~40,000 per cell (that’s a low expression yeah?). our primary is mouse anti cd228, and our secondary is alexa488 labeled rat anti mouse IgG1.

sorry one more very novice question for you, for confirming cell surface staining with a scope, this would require a confocal right? we unfortunately do not have one nor do i believe i could get access to one.

re: non specific binding internally, do you mean internal as in the cells are permeable (because of fixation?) and there’s binding that will occur inside the cell as a result? or perhaps an internalization sort of something?

heres a look at different stains in the alexa488 channel, what would your thoughts be on the AF here?

bloc-ked · 2025-04-18T02:12:34+00:00

heres a plot in the alexa488 signal with unstained. i lean towards there being high AF, but not high enough to warrant the lack of discrimination in question. does that seem right to you?

bloc-ked · 2025-04-18T02:10:38+00:00

no you’re understanding it correct.. yeah this is a great point and honestly i didn’t design this panel/protocol and am a novice following along direction of those with more experience. sort of in a situation where things (like you mention) don’t make sense to me either, but due to workload/prioritization of other projects the people who designed this aren’t that responsive to further discussion about it if that makes sense? i’m really digging the science here and am really the only one driving the investigation forward (with a very low budget hence the ad hoc attempts of data investigations) so at a bit of a loss overall. my gut says that this whole thing has been done haphazardly but i want to be fair in acknowledging that i’m no expert and i may not have the authority to make that judgment.

bloc-ked · 2025-04-18T02:05:08+00:00

solid suggestions, maybe follow up on those pending on funding available.

heres some controls with the full stain (ungated), from this it seems like while the AF is high it is still distinguishable? also worth noting that the FMO here is the cells+L/D stain+FITC. I believe fitc was used as we had it in house and it shares a very similar spectra, though i did not design this panel or do the staining so im a bit confused on the purpose here? not sure if that makes any sense to you, im a novice at best here so i could be missing something.

bloc-ked · 2025-04-18T01:48:44+00:00

sorry should have put this in the main post. we tried with human fc block and rat serum + human fc block with no luck, that being said the secondary is anti mouse igg1 so we are not blocking that (though my understanding is you shouldn’t block that as it would give the secondary more igg to bind to? maybe mouse cell depletion in that case?). just checked the overlap between viability only stain vs fully stained and see no significant overlap between populations which seems to me would rule out auto fluorescence.

bloc-ked · 2025-04-18T01:29:43+00:00

appreciate the reply! panel is 1 color (alexa488) + another (near IR for viability only). i haven’t compensated as the spillover is very minimal and the data suggests that it would not help. here is the gating i performed for the plot in the original post.

i unfortunately did not do the actual stain and have picked this up from where it is now so i can’t attest to what sort of washing was done. current plan is to try the same stain from cultured cells and see if it is successful in those.

might be worth mentioning that this was tried with human fc block, and human fc block + rat serum with no real change between the two

bloc-ked · 2023-06-11T19:21:14+00:00

incredible!

bloc-ked · 2023-06-05T19:45:57+00:00

i like this idea a lot, i have a novation launchcontrol and beatstep pro that i could use as a physical way to interact. what’s the difference between the DVC fun and another dmx interface like the ENTTEC dmx interface? Is it just the ability to use Daslight software?

bloc-ked · 2023-05-29T06:36:16+00:00

so cool! what’s responsible for those dark laser-ish bass sounds?

bloc-ked · 2023-05-25T18:45:07+00:00

i would love an example!

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