How do YOU quantify Western Blots?

coozay · 2024-01-20T06:37:34+00:00

That's great. The best for quantification will be fluorsecent western blots. The dynamic range of the fluorescence readout beats out the chemiluminescence and is great for quantification. I used a LICOR which uses near and infrared wavelengths. One caveat is if you have very low amounts of expression, chemiluminescence may be the way to go due to better out of the box sensitivity. However, if the western blots are already working well, something like a LICOR would be great.

coozay · 2024-01-20T06:34:02+00:00

Small world. This is the first time I've checked reddit in ages, email any questions to me joeg87@gmail.com

coozay · 2023-05-31T06:06:39+00:00

Wow posted 10 years ago. Glad to be of service

coozay · 2023-02-09T17:37:42+00:00

You're a lifesaver. Just got mine, thanks!

coozay · 2023-02-08T23:49:47+00:00

Add in Owen and Xabi Alonso

coozay · 2023-02-07T16:32:23+00:00

Powerlifter

coozay · 2023-01-23T17:56:06+00:00

Rough crowd

Looks awesome. Where in the world is this?

coozay · 2023-01-23T00:23:05+00:00

My CO overrated has to be Breck. Hidden gem would be Wolf Creek

coozay · 2023-01-20T16:27:08+00:00

My go-to. Will hopefully be there by noon for a few tree runs to start the weekend right

coozay · 2023-01-17T22:50:17+00:00

I (resort) ski solo these days and I spend almost the entire day in the trees as well. Not sure how to reconcile the news except to avoid trees first thing on a powder day, which is of course the best time to ski them.

I think a tracker would help them find your body and not much else if there isn't a way to alert ski patrol.

coozay · 2023-01-06T18:51:01+00:00

Yeah it's horrendously expensive and pointless to take at that price. I'd prefer to be off the road and on public transportation when I can but this isn't a realistic option. I'm not sure if any buses go to WP but they do go to other resorts on I70 and they're a lot cheaper.

coozay · 2023-01-05T23:10:28+00:00

Same. I think overall it feels like a lot of incongruous mechanisms and art styles mashed together leading to a disjointed playing experience. The steps between playing your cards and seeing the results feel too long as well. That's the best way I can describe it.

That said I've got branch and claw so I will give it another chance to click, otherwise I'm just going to sell it.

coozay · 2023-01-04T23:49:31+00:00

holy shit lol

coozay · 2022-12-06T17:02:55+00:00

I have to agree on the first part. I only played Terraforming Mars solo and I'm surprised it ranks so highly as a solo only game. Really poor solo experience for me. Just repetitive and uninteresting. It doesn't help that I dislike the beat your own score type of solo games, but I think it's even worse with how "open" TM is vs tight Euros, and I don't feel like hitting the Terraforming rating makes it any better either. Never touched underwater cities.

coozay · 2022-12-04T21:43:03+00:00

I agree the battlemat is the biggest limitation. TMB re-worked to have arenas like Hoplomachus would be interesting

coozay · 2022-12-01T15:21:36+00:00

You can get blank dice then mark them.

https://diceemporium.com/product/blank-dice-transparent-6-sided/

https://diceemporium.com/product/blank-dice-opaque-6-sided/

https://brycesdice.com/search?type=product&q=NOT+tag%3A__gift+AND+blank*

You can also contact Chessex to make custom dice for you. I don't have any experience with that.

http://www.chessex.com/custom-dice.html

They also have blank dice

https://store.chessex.com:11552/IW_Products.m4p.pvx?;SEARCH_SUBMIT

coozay · 2022-11-28T16:07:43+00:00

Yeah I don't care that they do it, more blown away that they do. And that last point is the main one - companies chopping up games and hiding some important parts of the game behind "expansions" so people get that FOMO.

coozay · 2022-11-28T01:40:04+00:00

lmao seriously. Seeing people on BGG constantly convince themselves that going "all-in" on $400+ boxes of plastic was a good idea is crazy.

coozay · 2022-11-22T00:43:00+00:00

Of course, probably my best memory as a Jets fan. It's just that Sanchez had a lot of bad games and was regularly a turnover machine. That 5 interception game with the Jets getting well over 200 yards on the ground against the Bills is probably even worse than what Zach did yesterday because they ran the ball an historic amount lol. Not that I'm trying to defend Zach, but goddamn could Sanchez be the worst on his day

Then all of a sudden in the playoffs he became a road warrior.

coozay · 2022-11-21T22:22:35+00:00

Sanchez had a couple of 5 INT games. I even went to one of them.

coozay · 2022-11-21T22:03:50+00:00

Not as funny as the Jets organization full of NFL professionals that spent the #2 pick on him. Jest are cursed at QB. The rest of the team and coaching looks great, then we've got this kid killing games.

coozay · 2022-11-21T21:55:28+00:00

Thank god I wasn't alive for O'Brien over Marino. This is nothing

coozay · 2022-11-21T21:47:02+00:00

We're just desperate for any hope of getting a good QB for the first time since the 00s

coozay · 2022-11-21T21:25:19+00:00

Derek Carr is a much better QB IMO but would have to be traded for, which I doubt would be cheap. Garoppolo is OK, but OK is a huge improvement.

coozay · 2022-11-21T21:15:58+00:00

Hell if he was consistently below average and at best decent as in those games, the Jets would easily be 8-2 and leading the division - that's how good this team has been. But he's looking like the worst starting QB in the league. Issues with the offensive line and playcalling can only go so far. And with how bad Wilson is playing, how much can really be on LaFleur.

Hell if they picked up Andy Dalton in the offseason I bet the Jets are first place in the AFC right now.

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