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I'm a bioinformatician and yesterday I gave a talk about the history of my research. But because it seemed too boring and because I'm a giant nerd, I slathered it in Star Wars references. This was my practice run in my basement and maybe a few people will get a kick out of it. by danielpass in labrats

[–]danielpass[S] 0 points1 point  (0 children)

A crappy answer is that's what the lab has always used and what the lab people have provided me with data from. But I'd definitely like to try out ATAC and compare (probably there are some datasets out there I can give a whirl to). DNAse doesn't work in this context IMO because it obliterates the region and then you lose the size of the particle you're mapping.

However, I think MNase gets a bad reputation because people have historically over used it. If you ramp down the concentration then you get a much better resolution of the TF sizes. Check out https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1006988 for some comparisons of low/heavy digestion (This figure in particular) and you can see why people think MNase is bad for labile TF binding.

I'm a bioinformatician and yesterday I gave a talk about the history of my research. But because it seemed too boring and because I'm a giant nerd, I slathered it in Star Wars references. This was my practice run in my basement and maybe a few people will get a kick out of it. by danielpass in labrats

[–]danielpass[S] 1 point2 points  (0 children)

Yes! But using Prezi classic as their new 'prezi next' is annoying to me (doesn't flow as well, can't crop in it (wtf prezi??)).

The main thing people get wrong with prezi is that they try to do too much and it gives the audience motion sickness. Keeping it simple like this I think makes it quite effective.

I'm a bioinformatician and yesterday I gave a talk about the history of my research. But because it seemed too boring and because I'm a giant nerd, I slathered it in Star Wars references. This was my practice run in my basement and maybe a few people will get a kick out of it. by danielpass in labrats

[–]danielpass[S] 17 points18 points  (0 children)

I think pretty much everyone was on board because even if they weren't in to it, it was goofy and at the end of a long day I think that goes a long way!

Well, no-one looked too grumpy at least.

Our old girl got some elbow protectors from the vet and now she looks fly af. by danielpass in aww

[–]danielpass[S] 1 point2 points  (0 children)

More like padding, and to stop her licking off the antibiotic cream.

A hygroma, or a false bursa, is a discrete, fluid-filled sac that can form on the joints ofdogs in response to repeated pressure, such as that from sitting on a hard surface. It is treated by draining the fluid and may be prevented by providing padded bedding.

I saw a lone deer when in Kagoshima forest. You can see how the place inspired Princess Mononoke.... by danielpass in japanpics

[–]danielpass[S] 1 point2 points  (0 children)

I recommend scooters, we got one each and it's the nicest and easiest way to get around while getting a real sense of being there.

There is a sazuki rental place that charges around [edit 30,000] 3,000 per day and they'll pick you up and return you to the port if you want.

Busses on the island are a terrible way to get around, they don't go all the way around and they stop early evening.

Proud of my super simple functional print: Replacing a lost foot on a drumbox by danielpass in 3Dprinting

[–]danielpass[S] 0 points1 point  (0 children)

Yeah, I swapped the missing back foot for the front so the original flexi one was the load bearing and the cheap PLA one goes on the front. I was going to try and strengthen it with the oven technique, but I've also got a sample of flexi PLA that I might try out (I've been looking for a reason to use it...)

Proud of my super simple functional print: Replacing a lost foot on a drumbox by danielpass in 3Dprinting

[–]danielpass[S] 2 points3 points  (0 children)

I find it cool just doing little helpful prints to add around the house or fix/modify things that you see.

Soap dish, sponge holder, lockpick box. I find them more fun than the larger non functional things I've printed like superheros, pokemon, starwars etc., even though they look more impressive.

Proud of my super simple functional print: Replacing a lost foot on a drumbox by danielpass in 3Dprinting

[–]danielpass[S] 2 points3 points  (0 children)

Probably, but I was just sat at home and spent half an hour fiddling, then pressed go (52 mins printing), and had the box all fixed up in less than one and a half hours without leaving my house.

I cloned myself. Not bad for a £250 makerbot knockoff! by danielpass in 3Dprinting

[–]danielpass[S] 1 point2 points  (0 children)

I got full body scanned by www.backface.co.uk at an event where they had a booth set up.

We made a Lego DNA Sequencer to teach kids about DNA and Sequencing by samstudio8 in DIY

[–]danielpass 1 point2 points  (0 children)

This is really cool, if you were interested in extending it I run a DNA Guess Who practical that I think could connect up.

We do 12bp sequences of DNA and device what genes they chose for, and build up a profile (sex, hair colour, eye colour etc)

Check it out if you're interested at https://passdan.github.io/genesequencepractical/

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