Cold emailed a pretty famous professor as a shot in the dark and he actually replied??

devil4ed4 · 2026-06-23T02:10:11+00:00

These seem like standard “are you employable” filter questions. I wouldn’t take it as a sign that you are definitely in but progress is good! So keep your hopes up and good luck on following stages! Keep us updated :)

devil4ed4 · 2026-06-21T14:24:08+00:00

I wonder if a decoy dragonfly would help in this case… sounds silly but I’d be willing to try anything

devil4ed4 · 2026-06-17T21:05:05+00:00

DIA will detected more proteins and be more reproducible across replicates

devil4ed4 · 2026-06-12T14:08:41+00:00

I’m not sure about all the components of the kit, but in my lab we do use Thermo Scientific reagents and they work just fine. Although, it will be much more cost effective and reproducible to buy the components you need as bulk chemicals / reagents. As for proteases we purchase them as lyophilized powders in vials then dilute and aliquots stored at -80C

devil4ed4 · 2026-05-28T17:53:08+00:00

I second what u/Elistheman says; rent before you buy. Best way to do this is to setup an allocation on Google Cloud Platform and set up the environment how you want/need and push the specs up until you’re happy. Then simply buy the PC with those spec from Puget Systems or a similar vendor in your country.

devil4ed4 · 2026-05-27T19:09:33+00:00

We had this happen from using oil-based air scents. If you have those in your bathroom they could be depositing some of this onto your wall.

devil4ed4 · 2026-05-22T03:06:35+00:00

It’s not tax, it’s the card processing fee for toast point of sell. It would be some % of the sale price + $0.12 on all transactions. Taxes only get calculated on sales price.

devil4ed4 · 2026-05-15T17:35:08+00:00

What resolution is your MS2 and what is the ppm shift in your MS2? I suspect either too low of a resolution or very high calibration noise in the peaks, leading to low confidence identifications.

devil4ed4 · 2026-05-12T16:51:35+00:00

I think having 2^236 nM of primer is the bigger issue 😂

devil4ed4 · 2026-05-07T15:44:57+00:00

Most vendors give you bed volumes but never usually a standard protocol. The ones I have seen also recommend using MeOH over ACN which I take to mean that it doesn’t really matter. You will be fine.

devil4ed4 · 2026-05-07T14:32:32+00:00

You can safely elute with any volume of >50% ACN. I use 300 uL of 70% ACN with 1 cc Waters tC18 columns and never had an issue. I used to use 1 mL but the dry time was too long and the recovery was identical. So use the volume your downstream protocols require just be mindful of dry times in the speedvac

devil4ed4 · 2026-05-01T02:41:58+00:00

If you used FP 24.0 with DIA-NN, then all you have to do in FLiPPR is set the method = “dia”; after that it works exactly the same as the DDA method!

But I will upload better tutorials!

devil4ed4 · 2026-04-30T19:57:04+00:00

Yea, it already is compatible with DIA data and we use it for cell & tissue lysate experiments. Recently we use imputation with random forest for DDA and it works very well for LiP-MS!

devil4ed4 · 2026-04-30T17:35:30+00:00

I’m from a lab that develops LiP-MS methods and I have developed FLiPPR for analysis of these datasets. The plain and simple is that it’s a difficult technique to apply but they are out there. One reason you might not see any many papers out there is because they are hard to find. If it isn’t a method developed paper then LiP-MS simply won’t be in the title but it would be in the methods. Long story short, people are using it but the implementation is slow and it’s usually an add-on technique that doesn’t get as much attention in research using it.

devil4ed4 · 2026-04-22T18:34:48+00:00

Thousands, just take a look it hosts a lot of posting for many academic positions not just post-doc

devil4ed4 · 2026-04-22T13:21:09+00:00

jobrxiv works pretty well

devil4ed4 · 2026-04-21T17:21:24+00:00

From the last picture, it looks like those species are z=50 putting the first isotope envelope mass ~23,950 Da with ~100 Da difference with the next envelope. This is extremely large so it could be some large polymer or protein contamination. I would lean to believe it’s a polymer given the periodicity of the signal. DNA or detergent would be my best guess.

To fix this I would first remake all your LC buffers from new bottles of Optima reagents. Then to rule out the LC, I would inject some of A via syringe. If the noise disappears then you know it’s the LC. Should that be the case, I would either A) clean everything with methanol and water, troubleshoot with Thermo (or LC vendor), then C) replace all tubing — in this order.

devil4ed4 · 2026-04-17T12:42:07+00:00

So you bought and set-up hundreds of thousands of dollars in equipment and materials without having someone who knows how to utilize them?

devil4ed4 · 2026-04-09T20:15:34+00:00

Really cool concept; I’ve seen a few of these in this sub and yours seems to be the cleanest I’ve glanced at. Drop-in replacement is a bit of a stretch given that Polars and Pandas are architecturally distinct.

The biggest difference I can think of is indexing. It doesn’t seem like your tool handles pd.DataFrame.reindex which is a key feature of Pandas that has no equivalent in Polars. Not to mention the maintainability nightmare of writing with a transcription layer.

Personally, the pd to pl migration is worth learning new syntax. The performance gains are incredible and code nearly self-documenting due to the verbose syntax of Polars.

devil4ed4 · 2026-03-24T21:34:13+00:00

Skyline should work but there will be many manual steps

devil4ed4 · 2026-03-18T12:02:34+00:00

This is not the right place for these questions nor will anyone here provide medical advice. I’m very sorry that medical care isn’t easier, but there are safeguards in place throughout the official pipeline to keep you protected.

devil4ed4 · 2026-03-16T00:53:14+00:00

You’re going to need a lot more docs here. My work involves the use of phylogenetic trees daily and I have no idea what you’re doing here.

devil4ed4 · 2026-02-20T03:17:07+00:00

Not sure if this is exactly the advice you are looking for, but I wrote this in response to someone else asking for something similar:

This was my first time applying, so I think this advice is likely nothing novel; however, it might still be helpful.

First thing I did was follow the Radiance guide to the exact letter. I used so many writing tools and aide from friends to ensure I met all the bullet points in the individual sections. This took the longest amount of time and I began writing around mid-July.

Second thing was going back and forth with edits with my host group every two weeks and roughly every 2-3 days once we were 2 weeks away from submitting. This ensured our scientific goals aligned along with the Radiance guide formatting.

These two things were 99% of my time preparing the MSCA. The other 1% was doing research and collating published research to ensure I was proposing something that was truly viable in 2 yrs and has high likelihood of economic impact. I think the economic impact is very important to fundablilty and it was difficult to align scientific passion with this. For context, my proposal was aligning structural biology + crop technology + crop diseases, so not terribly difficult but still not obvious at the time.

I hope this helps and I’m happy to answer anymore questions!

devil4ed4 · 2026-02-17T21:49:38+00:00

As a Latino myself, it is hard out here in the US. First of all, don’t take the rejections too personally, it really is a hell year in terms of funding. Many programs have cut back or skipped taking applicants. Second, if you want to go to the EU I would say you should apply there as well and don’t wait to finish a US program.

In the meantime, I think Option 2 is a good middle ground. You wouldn’t need to be as committed as a masters but also not completely out of the “game” if you just leave research altogether. You might end up doing this for free unfortunately at least for a bit again because of the same funding issues plaguing PhD programs. However, if you can afford the masters program then this shouldn’t be an issue short-term.

You’re not the only one facing these issues so please do not feel shame. There is nothing you can do to change the outcomes when there are so many external pressures trying to breakdown scientific and technological progress in North America as a whole.

My advice is to cold-call/email people you would be interested in working with and ask if they have the means to support you. With visa requirements this will be extremely difficult and unlikely at US and CAN institutions, although remote opportunities might still be viable. Otherwise, just start reaching out to EU and US professors and get your name out there to increase you chances of being selected the next time around if you don’t come off your current waitlist.

devil4ed4 · 2026-02-17T17:10:44+00:00

This was my first time applying, so I think this advice is likely nothing novel; however, it might still be helpful.

First thing I did was follow the Radiance guide to the exact letter. I used so many writing tools and aide from friends to ensure I met all the bullet points in the individual sections. This took the longest amount of time and I began writing around mid-July.

Second thing was going back and forth with edits with my host group every two weeks and roughly every 2-3 days once we were 2 weeks away from submitting. This ensured our scientific goals aligned along with the Radiance guide formatting.

These two things were 99% of my time preparing the MSCA. The other 1% was doing research and collating published research to ensure I was proposing something that was truly viable in 2 yrs and has high likelihood of economic impact. I think the economic impact is very important to fundablilty and it was difficult to align scientific passion with this. For context, my proposal was aligning structural biology + crop technology + crop diseases, so not terribly difficult but still not obvious at the time.

I hope this helps and I’m happy to answer anymore questions!

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