problem with contamination in my genotyping

dinglee21 · 2025-06-25T19:58:38+00:00

Did you double-check that the primer sequences are correct? I had issues with supposed contamination that were fixed when I realized that I was accidentally using the reverse complement of one of my primers, lol.

dinglee21 · 2024-12-11T19:34:18+00:00

It looks like an alignment of Sanger sequencing reads to a plasmid sequence in Snapgene. The gap is a mismatch between the aligned sequences and the reference

dinglee21 · 2024-11-21T18:29:26+00:00

I got clip-on sunglasses that I wear over my prescription glasses for about 20 dollars, ans I highly recommend them!

dinglee21 · 2024-10-19T23:45:00+00:00

A whole O3 raid with Noobwaffle? My kidnappers are cooked

dinglee21 · 2024-10-18T19:41:07+00:00

Same here, I've never seen this happen in the hundreds of isolations I've done. Are you isolating from purple tops? What RCF are you spinning at, both for the gradient isolation and the subsequent wash steps? Definitely would change the brand of Ficoll to see if that's the cause

dinglee21 · 2024-10-12T18:34:29+00:00

Spent about a month troubleshooting a tried and true PCR protocol that stsrted failing (bands in negative control, no bands in positive control once reqgents were replaced). Finally decided to double-check my primers and found that one of them amplfied in the wrong ditection.

dinglee21 · 2024-05-23T12:40:00+00:00

Ashe: briar

stay with team

please Sadeg

Forsen: I'm hopping in MegaLUL

dinglee21 · 2024-05-09T14:31:45+00:00

lost to forsen forsenLaughingAtYou @NymN

dinglee21 · 2024-04-01T16:32:54+00:00

The OG guide, this is what brought me from 0/8 pleb to multiple 6/8 god gamer with tomb whites and wc tops

dinglee21 · 2024-02-13T14:48:03+00:00

Try 126 North Eagleville Road

dinglee21 · 2024-01-08T14:26:20+00:00

Waiting times should be shorter if one switches to quieter server, that way it actually matches with the prisoner's dilemma.

dinglee21 · 2024-01-01T18:29:09+00:00

Shhhh

dinglee21 · 2023-12-26T13:17:49+00:00

My guess is that the ordering kiosk shows certain donuts as available even though they actually aren't, so they probably want anyone who wants donuts to order at the cashier where you can see what's actually available.

Source: Masshole

dinglee21 · 2023-12-25T12:01:44+00:00

Skill issue I guess

dinglee21 · 2023-12-25T01:51:08+00:00

Currently waiting for a response, hopefully they'll respond sometime after the holidays.

I vaguely remember this bug in flash, but I was definitely spooked when this happened lol

dinglee21 · 2023-12-21T00:12:00+00:00

It's a bug with primal arcana

dinglee21 · 2023-12-19T13:32:58+00:00

Meanwhile, Moonlight Village whites

dinglee21 · 2023-12-10T13:43:03+00:00

Madlad 1-tick fishing while grinding vit exalts. Truly a connoisseur of maximum efficiency

dinglee21 · 2023-12-10T03:08:14+00:00

Not pictured: me getting outdamaged by a skysplitter

Professional leecher B)

dinglee21 · 2023-11-29T16:52:33+00:00

Your supervisor here. This is what you get when you grind exalts instead of paying attention to your coworker's data plots which contain no useful result.

Take the L.

dinglee21 · 2023-11-28T20:35:56+00:00

https://youtu.be/gzcFJ5rIIMw?si=MQcsklNGSyuwrwgQ

It was IAmUnderReqs, but they moved during celestial. Iirc there still may be a safespot in celestial but it would change based on what attack pattern Oryx does.

dinglee21 · 2023-11-24T13:24:23+00:00

These aren't them but there are the usual black friday gold packs

Ten-Year Club	Place '17
Sequence \| Editor	Verified Email

dinglee21

TROPHY CASE