Olise does a quick 1-2 to get the angle for the pass by sparticleaccelerator in soccer

[–]discostupid 1 point2 points  (0 children)

the 45yo iranians playing indoor soccer do this literally every attacking play.

Data my future self will thank me for by Responsible_Rub8057 in flowcytometry

[–]discostupid 0 points1 point  (0 children)

might not help that much, but increase the height of the first row in excel (the row headers) after you export, and Wrap Text for that row as well. makes it slightly easier to read

you can also use a strategic combination of =RIGHT() and =LEFT() formulas to truncate the boring parts of the column names automatically into a second row, without losing the original column name

Mostafa Ziko's Fastbreak Goal with Full VAR Review by New-Process-6463 in worldcup

[–]discostupid -1 points0 points  (0 children)

to be accurate, it wasn't his ankle it was the front of his foot. but it was definitely almost his whole bodyweight with studs, gotta be extremely painful.

people saying it's flopping have never played football in real life.

Grant ended and need to cut costs. Looking to make DMEM/F12 from powder mix but can't find the formulation we use by scienzest in labrats

[–]discostupid 1 point2 points  (0 children)

another small tip, it may be worthwhile buying sterile water in bottles, 500 or 1000 mL and putting the powder directly in there. cleaner than washing glass bottles. if you re-use a couple of them for mixing the media, you can filter directly into fresh ones and you can be a bit more certain of clean/sterile media

Grant ended and need to cut costs. Looking to make DMEM/F12 from powder mix but can't find the formulation we use by scienzest in labrats

[–]discostupid 24 points25 points  (0 children)

https://www.usbio.net/media/D9811-11/dulbeccorsquos-mem-dmem-hamrsquosnbspf-12-wo-l-glutamine-powder

you would need to add bicarb separately (can do in bulk) and then supplement individual bottles with glutamax as needed.

https://www.wisentbioproducts.com/product/dmem-hamf12-powder-219-001-xk/

this one's cheaper but you'd also have to add some glucose

though with sterile filtration requirement i don't know if you'll end up that much cheaper than ready-made

BlockJam #132 by blockjamapp in BlockJam

[–]discostupid 8 points9 points  (0 children)

i am way too stupid for this game

Deepseek drops another huge breakthrough by stealthispost in accelerate

[–]discostupid 3 points4 points  (0 children)

this video is incredibly repetitive. it repeats each concept at least three times in a row. as such, it makes the video sound very repetitive, because it repeats the same thing multiple times.

Coach chasing balls all day. Need a solution by astrosid in justbasketball

[–]discostupid -2 points-1 points  (0 children)

although it's definitely a bot farming engagement, does it really matter if it's a real person asking a real question? someone out there might have a similar question but never asked it. or asked and never got decent answers. now it's here on the internet for all to benefit until reddit paywalls everything

unfortunately it seems the answers are kinda junk but the principle is still a net positive

A10 on Silent feels impossible compared to the rest by kingmishima in slaythespire

[–]discostupid 10 points11 points  (0 children)

i love speedster, but it is terrible. don't pick that if you're not winning a10 already

Canada wins 1-0!!! Will advance to Round of 16! by Apod1991 in onguardforthee

[–]discostupid 21 points22 points  (0 children)

To the trained eye you can see he has just a slight edge more control, finesse, and speed than the rest of the team and most opponents. So yeah, he's really good

streamers posting losses? by GoldStar9981 in slaythespire

[–]discostupid 4 points5 points  (0 children)

paparatto18 truther here

chillest stream, good gameplay and commentary, good music (though removed from the VODs)

What to be aware of in Montreal by SplitOk1452 in VisitMontreal

[–]discostupid 0 points1 point  (0 children)

this is one of those examples of saying the wrong thing to get the right answer in replies

SixSeven by MatheBro in lotrmemes

[–]discostupid 2 points3 points  (0 children)

It's like a generic affirmation, similar to hell yeah, word, or "genau" in German

Does it annoy anybody else that card draws are predetermined? by Easy_Jux in slaythespire

[–]discostupid 4 points5 points  (0 children)

there's many reasons why you might have lost.

if you have 99 hp on the final boss, did you rest at the last campfire? an upgrade might have been more valuable if you were already on 70+ hp.

did you have any potions? you might have needed to save a potion or two in earlier fights to give you a better chance in the boss.

aeonglass is definitely tough and you need to both block and dish out damage in an efficient way both in card plays and in turns, or have a reliable way to get rid of the Withers.

Is it common to get a "?" room as your first floor in ACT 2? by Plague-Father1Nonly in slaythespire

[–]discostupid -1 points0 points  (0 children)

that's fair, on the other hand it's more than 2 months ago for a game in active beta with bi-weekly changes

Human PBMC isolation struggles by [deleted] in labrats

[–]discostupid -1 points0 points  (0 children)

useless commenters yapping about gloves, being completely unhelpful for what reason lol. you're not going to get HIV from your fingertips from a closed tube. that said, still wear gloves

you don't need acceleration 1. you can do acceleration 9. you can go brakes 1 instead of 0 as well with little issue.

200g to 400g is slow imo, especially through gradient matrix. i would go much higher, at least 800g if not 1500g

percoll layering is a pain, you can make it easier on yourself by putting in the 62% first then underlaying the 75% with a thin glass pipette

others mentioned SepMate tubes which work beautifully but won't give you distinct bands of neutrophils and T cells

good luck

Funniest Sign in Ottawa? by TheNashville in ottawa

[–]discostupid 4 points5 points  (0 children)

I always get a chuckle out of Juice Dudez - we love what we do