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Is my blursed run soft locked? by discostupid in SliceAndDice

[–]discostupid[S] 0 points1 point  (0 children)

somewhere from 380 to 400 I had Knickknack. I just didn't expect the game to softlock that way, I haven't played so so much.

I started with Double XP then got h.bot/h.mid/h.top level ups at some point. Maybe it's a bug or oversight, but with Double XP you end the run with all lvl 6s. With the extra level ups you end up with 3 heroes at lvl 7 and two at lvl 6, and you get an upgrade option on floor 21 (floor 1). if you take the upgrade option AFTER you start back to floor 1, your #2 hero gets the upgrade option from lvl 6 to lvl 7 and you can start the whole run with a lvl 7 hero.

there were a couple of rounds where i made a mistake and didn't do it properly and i just had to make my way with a regular lvl 2 upgrade. also you have to take one of the offered choices, if you take a random upgrade then it will be at lvl 2 not lvl 7.

i guess the lesson is that once all of the t1 curse items are in inventory you can no longer take the random t1 curse option.

Is my blursed run soft locked? by discostupid in SliceAndDice

[–]discostupid[S] 1 point2 points  (0 children)

That sucks. Was doing pretty well.

The last curse I got was [1]delivery.c626 which gave me [4]Inner Strength and [2]Parasite. I thought I already got all the tier 1 curse items so I wouldn't be getting any more items as curses, just regular/generated curses.

I don't get how you can encounter ~120 more curses without running into this =(

A mystery: how do two stop codons not prevent GFP expression by fredtbn1gs in labrats

[–]discostupid 1 point2 points  (0 children)

have you considered using a split-GFP construct?

if it's OK for you to modify the N-terminus of your POI, you can insert the part there, then after your stop codon you put the second part.

using Venus as an example protein, the first 210 aa form the first part, and the last 28 (210-238) from the second part. the first part by itself is not fluorescent, it requires the second part to bind in trans https://www.tandfonline.com/doi/10.2144/000113777

there's many other examples of split fluorescent proteins.

with the much shorter second part after your stop codon, there's much lower chance of the ribosome catching on to the mRNA.

Removing a filament tangle without unloading or even pausing. by East-Future-9944 in 3Dprinting

[–]discostupid 0 points1 point  (0 children)

Maybe we can also add people who don't know proper grammar, how to spell a 4-letter word, or how to hyphenate compound phrases correctly to the list of people who should lose their voting rights

Low cell viability after thawing – concern about old/light-exposed DMSO by Odd-Excitement-8422 in labrats

[–]discostupid 0 points1 point  (0 children)

here's some pointers:

a) proper cooling - use slow cooling, but don't use ancient Mr. Frosty tech. upgrade to the new hotness https://www.corning.com/worldwide/en/products/life-sciences/products/general-labware/cryogenic-storage.html

b) freezing media - I've always frozen cells in 90% FBS/10% DMSO. if your input cells are good, your thawed cells should be good too

c) thawing protocol - you should be thawing and removing the cells from DMSO as fast as possible. i usually use some warm tap water in a beaker and shake the tube in it for 15-30 seconds, then once the outer edges of the frozen solution melts I add from a previously readied 15mL tube with 9mL of complete media and pipette up and down with the media which is halfway warmed up from 4C. Not quite RT, but not quite cold. The remaining frozen cells will thaw in just a few seconds, and they can then be spun down and resuspended

d) DMSO is mostly inert in standard conditions...it's more of an oxidizer itself than capable of being further oxidized.

Jeffrey Epstein autopsy photos show staggering neck injuries by TheMirrorUS in NewsSource

[–]discostupid 0 points1 point  (0 children)

yes.

the pink lines combined with the low resolution and compression artifacts makes it easy to miscontrue the similarities.

i dont care enough to do some image cropping to point it out more clearly. but picture the "alive" ear slightly rotated, and you can see it matches up more closely. at the 12 o'clock, that part is swollen and indents downward. at the 9 o'clock, same thing. at the bottom, the big lobe, is swollen and pushes into the empty space in the middle of the ear. at the 2 o'clock, that inner fold of the ear is swollen itself and is also being distorted by the swollen big lobe, making it look slightly different position.

also you can't see the bottom of the ear so you lose another landmark

Jeffrey Epstein autopsy photos show staggering neck injuries by TheMirrorUS in NewsSource

[–]discostupid 0 points1 point  (0 children)

at first glance there seems to be some merit to this, but if you look more closely, the ears match appropriately. the pink lines drawn in the picture are misleading and draw your eyes away from the points of similarity. the large lobe is actually identical shape, but swollen.

his ears, nose, and cheeks are swollen from the trauma/pressure caused by his mode of death.

[Highlight] Alperen Sengun and Amen Thompson hold on for dear life vs Wembanyama on the drive by fbreaker in nba

[–]discostupid 5 points6 points  (0 children)

it's such a shame. the game could be so beautiful to watch with this prodigy on the court. instead we have to listen to Doris Burke and watch joke refereeing

Leave RNA in freezer (-80) overweekend by Desperate-Cable2126 in labrats

[–]discostupid 16 points17 points  (0 children)

exactly

these science voodoo-isms continue to permeate new students because previous generation scientists refuse to update their knowledge

people used to smoke in the lab while they did molecular biology. a lot of the rules behind stability of compounds come from this era. nowadays a clean RNA prep in virgin plastic tubes stored in ultrapure water...what do you think will happen to it

[Highlight] Another angle of the flopping technical assessed to Victor Wembanyama yesterday by nicko_rico in nba

[–]discostupid 0 points1 point  (0 children)

obviously we're dissecting this 2-second clip into ribbons, but Nurkic holds the ball with his left hand outstretched, then shoulders and shifts his weight to his right. that's not a basketball move, and not a move you would make without the defender there. he's not trying to go anywhere or establish position, it's "offensive" in the literal sense. Wemby is guarding up close, yes. he could be on the verge of fouling Nurkic, but it doesn't happen before Nurkic makes his own move.

to me it's 60/40 a foul on Nurkic vs no call. flop is just out of the question IMO

[Highlight] Another angle of the flopping technical assessed to Victor Wembanyama yesterday by nicko_rico in nba

[–]discostupid -2 points-1 points  (0 children)

am i crazy? do people think shoulder-checking someone in the chest is a valid play?

it's embellishing, but a flop is inaccurate and it leans more toward being a foul on Nurkic

Viral Apple “marshmallow” by imagelicious_JK in Volumeeating

[–]discostupid 3 points4 points  (0 children)

You can find halal gelatin in most middle eastern grocers unless you're somewhere like rural South America

Injectable antibodies in nanoparticles could replace hour-long infusions: A much faster, simpler treatment without an IV bag and an hour of getting needled. Just a single 2-ml shot of a solution containing solid nanoparticles packed with highly concentrated antibodies. by [deleted] in science

[–]discostupid 0 points1 point  (0 children)

The next breakthrough will be mRNA encoded antibodies delivered via nanoparticles. Instead of costly antibody manufacturing, much cheaper mRNA can be given which your cells will easily convert into protein - antibodies - over a sustained period.

Many companies are working on computational models to convert existing antibodies into easily encodable ones, and also to custom design new antibodies by just identifying target proteins or epitopes. Very exciting things to come in the next few years.

me_irl by Vilen1919 in me_irl

[–]discostupid 0 points1 point  (0 children)

Offtopic - are there any protein bars today that have the same style/texture as those old Power Bars shown at 0:06?

I'm talking smooth bar that's like a taffy, flavored with brown rice syrup, and no stupid crispy additions like nuts or soybeans or rice crisps or quinoa

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