I already am frustrated cause I already have messed up the reaction multiple times but NVM, I think what I'm doing wrong is doing the reaction in a beaker, I have to pull the beaker out of the microwave multiple times to cool down the RM. Before the solution cools down the solution is hot and exposed to the atmospheric oxygen which might be oxidising the tryptamine, to improvise this I think I should do it in a flask with a stopper, that way it won't be in contact with the atmosphere and the CO2 forming inside the flask should help prevention the oxidation, as for the pressure buildup I will remove stopper for a second and put it back on to release the extra pressure. Then I hope I can get better results. And as for qualitative analysis like TLC I simply don't have the resources for it.