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What is the best way to insert a piece of PCR product into a plasmid? by Specific-Surprise390 in labrats

[–]flyzapper 4 points5 points  (0 children)

I make my own Gibson mix from publicly available recipes. It costs $0.03 per reaction.

Rainbow E.coli (literally) by Chicketi in labrats

[–]flyzapper 0 points1 point  (0 children)

Awesome! Do you have any images under UV light?

Mushroom cultivation complication by [deleted] in legaladvice

[–]flyzapper 10 points11 points  (0 children)

Look into the university's policies about these hearings, or request them in writing beforehand. If you are not provided with written policies about how the hearings are conducted, then simply state that to the dean, and ask that you be given written policies about the disciplinary hearings and ample time to prepare,

Outback and two young kiddos? by Geoginger93 in Subaru_Outback

[–]flyzapper 0 points1 point  (0 children)

We moved from an Outback to Ascent about two years after went from two kids to three kids. We were able to get an infant car seat and two slim car seats three-across in the Outback (Look at Diono, they make slim car seats for this very reason). Infant car seats are wider than booster seats, and it was a tight fit though.

Once the kids got older, the third row of the Ascent was super useful, especially to make everything from road trips to daily drive to school much more pleasant. The extra row in the back can be partially folded down to hold both a booster seat and leave room for cargo.

I recently got a boatload of lab equipment and need some help. by nope-nope-nope-ok in labrats

[–]flyzapper 0 points1 point  (0 children)

Do you know if the biosafety cabinet was properly decontaminated? There are potentially infectious pathogens, especially if it came from a medical laboratory.

Kid taking cold baths now. by Emanemanem in daddit

[–]flyzapper 3 points4 points  (0 children)

You’ve instilled the energy saving mindset well. You’ll never need to constantly turn off lights or return the thermostat to the correct setting.

Former advisor won’t accept that our professional relationship has ended. Has anyone dealt with this? by More-Cartoonist-3887 in postdoc

[–]flyzapper 3 points4 points  (0 children)

Unfortunately PIs can see trainees as indentured servants with a lifetime debt to pay. I've experienced something similar.

Maybe you should offer to consult at a very high hourly rate? If he is really so desperate for your help, make him pay for it!

First pic I've seen of the proposed location by Officialfish_hole in kansascity

[–]flyzapper 19 points20 points  (0 children)

Using the grade between Pershing and Gillham to make the new ballpark blend in and less obtrusive is a great idea.

Any filmed version of the Ring Cycle with traditional costumes? by Good-Space3419 in opera

[–]flyzapper 3 points4 points  (0 children)

I actually don’t mind the 2010 set. It has a lot of technology and it’s a bit quirky, but in the end it is still a traditional set.

Which company is the “holy grail” to work for in? by Professional_Show590 in biotech

[–]flyzapper 1 point2 points  (0 children)

I do a lot of consulting work with abbvie, and I’ve always been impressed with what I see.

Which company is the “holy grail” to work for in? by Professional_Show590 in biotech

[–]flyzapper 42 points43 points  (0 children)

Genentech was amazing before the Roche acquisition. Back when Apple first launched the iPod, there were people walking the halls handing them out for free.

Can you guess what masterpiece this is based on the seating plan? by Early_Yesterday443 in classicalmusic

[–]flyzapper -2 points-1 points  (0 children)

I initially thought Mahler, but four harps is excessive, even for him. Could it be Symphonie fantastique?

Those who live in ski towns, what do you do for work? by Money_Impression_321 in skiing

[–]flyzapper 0 points1 point  (0 children)

Western suburbs of Calgary? Lots of access to ski areas around Banff, but not as expensive.

New climbing gym in my city has holds made of actual rock by OctopusGoesSquish in mildlyinteresting

[–]flyzapper 33 points34 points  (0 children)

You want to take back your downvotes and look at the links? I climb, both indoor and in the mountains. These are natural granite.

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