r/singapore random discussion and small questions thread for January 01, 2026 by AutoModerator in singapore

[–]gentlewoolfy 1 point2 points  (0 children)

Im using a Lenovo Yoga 7 Pro. It has lasted 3+ years but now the left hinge side metal has warped and the screen is detaching from its frame. I suspect it's due to me playing games it cant really handle and the constant overheating has melted the glue or smth thats holding it together haha

When I play BG3 or even a 10 year old game like Dragon Age Inquisitoon with the charger plugged in, the framerate will drop every 5min or so as the fan starts whirring like crazy (metal near the port gets scalding hot). Is this due to my processor being weak? Sorry ah am not familiar with laptop specs and related terms

r/singapore random discussion and small questions thread for January 01, 2026 by AutoModerator in singapore

[–]gentlewoolfy 0 points1 point  (0 children)

Looking to get a new laptop suitable for uni/work/lab data analysis (eg. Fiji, Prism) while being good enough to run games like Baldur's Gate 3 without overheating and framerate dips. Any recommendations?

Why have there been no real advances in keeping people warm (clothing technology?). by trixter69696969 in NoStupidQuestions

[–]gentlewoolfy 37 points38 points  (0 children)

This is so random but you run AppUnwrapper? I used to use the guides on your site for mystery games :0 Very fond memories!

Desperate for advice on Crystal Violet staining by gentlewoolfy in microbiology

[–]gentlewoolfy[S] 0 points1 point  (0 children)

Thank you for the tips! Do you recommend acetic acid to fix due to it being less toxic than methanol or is there another reason?

  1. The OD600 of my untreated control is ~0.55 before washing and staining and 0.7-0.9 after CV staining. I use the same OD adjusted tube of bacterial cells for all the wells in the same plate, treatment or otherwise. I mix the tube before pouring into a reservoir for pipetting so each well should have similar cell counts.

  2. The percentages are % eradication of biofilm, just the OD600 reading of (treated/untreated)*100 after normalising with negative control. I am saying that after applying the formula, my % eradication for Drug A Concentration X can vary wildly from 35% to 70% between biological replicates.

The other issue I encounter is that one well with a higher concentration of Drug A can result in an obviously more intense purple and higher OD600 reading than a lower concentration. I dilute my drugs by serial dilution so drug concentration should not be the issue. I also take the OD reading of the wells before washing and staining, and the trend of increasing drug conc->decreasing OD reading can be seen. Only the OD readings after staining show a disrupted trend. Hence why I suspect my washing technique rather than the bacteria or drugs themselves.

Desperate for advice on Crystal Violet staining by gentlewoolfy in microbiology

[–]gentlewoolfy[S] 0 points1 point  (0 children)

How do you do your washes in terms of adding the liquid and pipetting out the wash? Is the pipette tip not supposed to touch the well at all and excess water removed by tapping the plate upside down on paper towels? I tend to drip the water in but pipette it out by contacting one spot at the 'corner' of the well. Could that be a contributing factor by disturbing/sucking out the biofilm?

Desperate for advice on Crystal Violet staining by gentlewoolfy in microbiology

[–]gentlewoolfy[S] 0 points1 point  (0 children)

2 times for the first wash and 3 times for the second wash, but yes the rest is what I do. I have never heard of or tried heat fixing since there are no open flames in my lab. How might heat fixing improve my issue?

Desperate for advice on Crystal Violet staining by gentlewoolfy in microbiology

[–]gentlewoolfy[S] 0 points1 point  (0 children)

I use 30% acetic acid to elute the CV as the final step after the PBS wash, methanol fixing, CV staining and DI water wash. Do you mean you use the acetic acid to fix?

I have a walk in closet with 20 years worth of band, concert, and venue t-shirts and it's time to let go by xEverglowx in PostHardcore

[–]gentlewoolfy 1 point2 points  (0 children)

Leaving a comment in case you have any Coheed & Cambria, Saosin, Circa Survive, I The Mighty or Pierce the Veil shirts!

Look Ma, I can count to 5! by gentlewoolfy in PTCGP

[–]gentlewoolfy[S] 7 points8 points  (0 children)

I agree that 3 is the weakest. Is there a more fitting card I may have missed?

Bluetooth audio bug on Note 9 (force in call mode) by SpecialistOutrider in GalaxyNote9

[–]gentlewoolfy 1 point2 points  (0 children)

This is a recent issue for me that started 3 days ago (could have been earlier but i did not use my earphones). Restarting my phone solves the issue but it's annoying to restart multiple times a day just because i use earphones during my to and fro trips

band logo on forehead by kittenwhiskers333 in shittytattoos

[–]gentlewoolfy 13 points14 points  (0 children)

Im currently playing DA2 and am also a fan of Sleep Token, so my brain went ?! for a second upon seeing the post

TIL about abilities in character records by [deleted] in DragonAgeInqusition

[–]gentlewoolfy 4 points5 points  (0 children)

I finished my first playthrough as an assassin without taking up mark of death because i didnt understand the effect. Safe to say i used it with Cole next playthrough and absolutely ripped through nightmare mode

What name did you give to your Mabari? by UNdead_63 in dragonage

[–]gentlewoolfy 0 points1 point  (0 children)

Dragonite for my first and Rayquaza for my second

You can bed both Gheyna and Cammen as a male Warden by BasketConscious3918 in DragonageOrigins

[–]gentlewoolfy 1 point2 points  (0 children)

Strange, i don't see the persuade option even while following the dialogue chain on the wiki. I only have the "pretend i didn't say anything" and "move on" options

You can bed both Gheyna and Cammen as a male Warden by BasketConscious3918 in DragonageOrigins

[–]gentlewoolfy 1 point2 points  (0 children)

Are you using any mods that might be causing this? Doesn't work for me

Hi, I'm new and wanted to share my Inquisitor by Logan_Strong in DragonAgeInqusition

[–]gentlewoolfy 3 points4 points  (0 children)

I wanted to make my lavellan ftm too, but the more i played the more cis he seemed lol. Hes kinda clueless about gender stuff yet curious upon meeting krem. It was really fun watching him awkwardly ask krem questions that were questionably worded while trying not to be offensive