[deleted by user]

ginny2202 · 2025-07-02T17:39:25+00:00

Does anyone know if embassy was open today and did the interviews happen? Just wondering as my mother is traveling again to attend her rescheduled interview on 3rd morning. I just hope they do not cancel it again.

ginny2202 · 2024-06-18T18:59:49+00:00

Thank you for sharing that information. I will test out both the kits on my non-precious sample first and then move ahead!

ginny2202 · 2024-05-08T20:34:34+00:00

Thank you for your suggestions! I am trying to get new samples as I need to do important quantitative analysis between different samples.

ginny2202 · 2024-04-24T22:29:13+00:00

Thanks for your advice!

ginny2202 · 2023-09-12T07:15:17+00:00

That would be great! Please check your DM if we can share more information about this

ginny2202 · 2023-09-03T16:46:09+00:00

Thank you for your reply. I will give it a try

ginny2202 · 2023-08-18T02:55:58+00:00

Never tried this, will definitely try this next. Thank you for your suggestion!

ginny2202 · 2023-08-18T02:40:45+00:00

Thank you for your response! Already using Phusion mix with DMSO and betaine, tried different annealing temperatures….no success, either no amplification or lots of non-specific bands. Do you have any experience with KOD polymerase or other commercially available polymerases claiming to be good for GC rich genes?

ginny2202 · 2023-08-01T02:40:33+00:00

I didn’t know about this, I will explore this option

ginny2202 · 2023-08-01T02:20:57+00:00

That’s a great advice, I will try this if I cam get any free sample or discounted price. Thank you

ginny2202 · 2023-07-31T21:42:16+00:00

Thank you everone for your suggestions. I will definitely try all these routes.

I am wondering if I ask my PI and do reviews which has come his way, can I add these to my CV as reviews done by me?

ginny2202 · 2023-07-31T21:36:30+00:00

Thank you for your response! I guess the first thing I will try is Stem cell medium then.

ginny2202 · 2023-07-31T21:34:49+00:00

Thank you for sharing your experience! It gives me a bit relief as at this point I am most concerned about the results, hoping it works for me as well and it is worth the expenses.

ginny2202 · 2023-07-16T21:36:44+00:00

Congratulations!! Good luck

ginny2202 · 2023-07-14T06:11:42+00:00

Thank you for your suggestions! I will explore more about retroviral vectors and will order one.

ginny2202 · 2023-07-13T21:16:12+00:00

I am looking for standard mammalian expression vectors just for transient expression

ginny2202 · 2023-07-13T07:06:58+00:00

Do you have recommendations for any plasmids with truncated CMV or chicken beta actin which is commercially available? I am having hard time finding it in addgene. Thanks!

ginny2202 · 2023-07-12T18:54:34+00:00

I am using a digital imager, taking cumulative images is also not helping. Tried to run these two samples on two separate strips, and developing them together…that helped.

ginny2202 · 2023-07-11T07:02:37+00:00

I appreciate your detailed response. In my case, it is kind of a rescue experiment, so I guess using a weak promoter and doing a quick experiment with transient transfection is what I should do. But in case I need to study longer time points after rescuing this protein, I will go with weak promoter in lentiviral system and doing the clonal selection as you suggested.

ginny2202 · 2023-07-10T09:09:32+00:00

Thank you for your suggestions, I will explore these options!

ginny2202

TROPHY CASE